Rice bran was produced from rice-hulling process. Rice bran was contain
high starch, therefore it was used to saccharification process. The aim of
this research was to study the optimum concentration of rice bran to
produce reducing sugar on saccharification process by Rhizopus oryzae. The
100 mL-rice bran medium (5, 10, 15, 20, 25, and 30%) were sterilized at 1210C,
for 15 minutes and inoculated with R. oryzae (± 2x106 cfu) and incubated for
3 days. The concentration of reducing sugar, starch, pH value, and biomass
were analyzed everyday. Concentration of starch was decreasing during
saccharification process. However, the concentration of reducing sugar was
maximum at first day saccharification. The optimum concentration of rice
bran for saccharification process was showed by maximum production of
reducing sugar (15,347 mg/mL); it was in 20% concentration of medium. The
pH value was decreasing during saccharification process, and the biomass
was reversed.
Keywords: rice brans, Rhizopus oryzae, saccharification, reducing sugar
