The aims of the research were: to know species diversity of fern (Pteridophyte) from Pangajaran, Wonosalam,
Jombang, to know fern species containing volatile oil, to know concentration and percentage similarity of substances and
characteristics of the substances containing in the oil, and to know the structure of cell producing volatile oil in trees and
leaf of the fern. Fern diversity was studied by field survey, volatile oil concentration measured by hydro-distillation
followed with gas chromatography to further know the components in the oil, while structure of the cell producing volatile
oil was detected cross section of the trees and leaf for microscopic analysis. Based on the data and analysis resulted can
be concluded that there were 13 fern species in Pangajaran. Two of the 13 species were confirmed as producing volatile
oil, Pteris beaurita Linn. and Cyathea contaminans, that were produced volatile on their leaf only. Concentration of
volatile oil of leaf P. beaurita was 0,005%, while in C. contaminans 0,01%. Percentage similarity of the volatile oil
between two species based on its Retention Time (RT) was 2,5%, at the RT point of 21.247 in P. beaurita and at RT point
of 21.294 in C. contaminans. Percentage similarity of both species based on morphological characters was 36.36%.
Location of volatile oil producing cells in both species of fern was spreadly dispersed in schlerenchyme tissue and in
mesophyl tissue of the leaf.
Keywords: volatile oils, fern diversity, Wonosalam, Jombang
