Conditions have been developed for transforming protoplast of the Acremonium endophyte by PEG 4000 and electroporation. Transformation by PEG exhibited a higher number of tranformants than by electroporation. Intregation of iaaM gene into the genome was examined by PCR and Southern blot hybridization analysis. PCR product showed that transformants banded at around 1.7 kb corresponding to the size of iaaM gene. Hybridizationn of the digest of genomic DNA with iaaM gene as DNA probe showed that the number of hybridized band signals was different between tranformant and non-transformant. These result might indicate that PEG is an effective method for the transformation of Acremonium endopyhte and that there are repeated copies of the iaaM homologous sequences in the genome of Acremonium.
Key words: Acremonium sp., endhophyte, transformation, iaaM gene, hph gen
