Floodplain soils are often exposed to pesticides but are seldom recognized as a subject to environmental fate studies. We incubated a floodplain and an agricultural soil with 14C-position-specific labeled Glyphosate at different soil moisture contents. Our study confirms the potential of Glyphosate degradation in a floodplain soil. However, the agricultural soil, with a continuous history of Glyphosate use, was able to mineralize Glyphosate 3 times faster compared to the floodplain soil. Consequently, the mineralization kinetics of the pesticide differed significantly between the two soil types, with a half-life of 100 days for the agricultural soi