Induction of callus and plant regeneration in Vicoa indica

Abstract

Callus cultures were initiated from the stem and leaf explants of aseptically grown Vicoa indica. A simple method is described for plant regeneration from callus and the rapid multiplication of the plants thus obtained. Callus initiation was optimum in Gamborg B5 (B5) basal medium containing either $2.0 mg\hspace{2mm} l^{-1}$ naphthaleneacetic acid (NAA) with $0.2 mg\hspace{2mm} l^{-1}$ kinetin (Kn) or $2.0 mg\hspace{2mm} l^{-1}$ 6-benzylaminopurine (BAP) with $0.2 mg\hspace{2mm} l^{-1}$ NAA. The calli initiated on B5 medium were able to proliferate on both Murashige and Skoog (MS) and B5 basal medium. Shoot primordia were obtained from greenish callus on passage to B5 basal medium containing $3.0 mg\hspace{2mm} l^{-1}$ BAP and $1.0 mg\hspace{2mm} l^{-1}$ Kn. On further subculture onto B5 medium containing $0.2 mg\hspace{2mm} l^{-1}$ Kn the shoot primordia developed into plantlets