CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
research
Reconstitution of a 26-Subunit human kinetochore reveals cooperative microtubule binding by CENP-OPQUR and NDC80
Authors
Philip Auckland
Muriel Erent
+12 more
Alex C. Faesen
Yu-Chih Lin
Stefano Maffini
Andrew D. McAinsh
Andrea Musacchio
Satyakrishna Pentakota
Marion E. Pesenti
Arsen Petrovic
Daniel Prumbaum
Stefan Raunser
Charlotte M. Smith
John R. Weir
Publication date
1 January 2018
Publisher
'Elsevier BV'
Doi
Cite
Abstract
The approximately thirty core subunits of kinetochores assemble on centromeric chromatin containing the histone H3 variant CENP-A and connect chromosomes with spindle microtubules. The chromatin proximal 16-subunit CCAN (constitutive centromere associated network) creates a mechanically stable bridge between CENP-A and the kinetochore's microtubule-binding machinery, the 10-subunit KMN assembly. Here, we reconstituted a stoichiometric 11-subunit human CCAN core that forms when the CENP-OPQUR complex binds to a joint interface on the CENP-HIKM and CENP-LN complexes. The resulting CCAN particle is globular and connects KMN and CENP-A in a 26-subunit recombinant particle. The disordered, basic N-terminal tail of CENP-Q binds microtubules and promotes accurate chromosome alignment, cooperating with KMN in microtubule binding. The N-terminal basic tail of the NDC80 complex, the microtubule-binding subunit of KMN, can functionally replace the CENP-Q tail. Our work dissects the connectivity and architecture of CCAN and reveals unexpected functional similarities between CENP-OPQUR and the NDC80 complex. [Abstract copyright: Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
MPG.PuRe
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:pure.mpg.de:item_3150466
Last time updated on 20/08/2019
Warwick Research Archives Portal Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:wrap.warwick.ac.uk:108553
Last time updated on 25/09/2018
MPG.PuRe
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:pure.mpg.de:item_3002383
Last time updated on 15/06/2019
MPG.PuRe
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:pure.mpg.de:item_3002931
Last time updated on 15/06/2019