Korhonen, Anna

Laukkanen, Miikka

Lehto, Tarja

Repo, Tapani

Silvennoinen, Raimo

English

Jukuri

Metla/3595/EPur/04.20131)  The Finnish Forest Research Institute, Joensuu unit, P.O. Box 68, FI-80101 Joensuu, Finland, tapani.repo@metla.fi2)  School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI-80101, Joensuu, Finland3)  University of Eastern Finland, Department of Physics and Mathematics, P.O. Box 111, FI-80101 Joensuu, FinlandAnalysis of mycorrhizal colonisation of Scots pine roots by electrical impedance spectroscopyTapani Repo1), Anna Korhonen1,2), Miikka Laukkanen3), Tarja Lehto2) and Raimo Silvennoinen3)IntroductionThere are no non-destructive methods for measu-ring mycorrhizal colonization of plant roots. We studied if colonization by symbiotic mycorrhizal fungi would affect electrical impedance spectra (EIS) of Scots pine (Pinus sylvestris L.) roots in non-destructive measurements.  Material and methods• The seedlings were raised in growth room conditions. The roots were inoculated with mycorrhizal fungus Hebeloma sp. (H) or with Suillus luteus (SL) or left as non-mycorrhizal control (O) (Fig. 1). • EIS of the root systems were measured after 19 weeks with a three-electrode measuring setup (Fig. 2). • Normalized EIS were analysed by a classifi-cation method using CLAFIC algorithm that employs principal component analysis (PCA). Results• There was no difference between the treatments in the original or normalized data (Fig. 3). • In the CLAFIC-analysis, O and H treatments could be classified correctly at the dimension >10 and SL treatment at the dimension >15 (Fig. 4). 0 5 10 150510152025DimensionNumber of samples  (A)Non−mycorrhizalHebelomaSuillus 0 5 10 150510152025Number of samplesDimension(B)0 5 10 150510152025DimensionNumber of samples(C)0 5 10 150510152025DimensionNumber of samples(D)0 5 10 150510152025DimensionNumber of samples(E)0 5 10 150510152025DimensionNumber of samples(F)Fig. 3. The normalized real (A) and imaginary (B) part of roots by mycorrhizal treatments (mean of 24 spectra)Fig. 2. Experimental set-up to measure electrical impedance spectrum of the root system. Fig. 1. Examples of non-mycorrhizal and mycorrhizal roots (up) of the pine seedlings (below).Fig. 4. Classification of the real (A-C) and imaginary (D-F) part of EIS of roots by mycorrhizal treatments  in three-class problem. Total number of spectra n=142.ConclusionsThe seedlings by fungal treatments could be clas-sified according to the current delivery through the root system electrolytes sensed by EIS. Cur-rent flow is more dispersive through SL than H or non-mycorrhizal roots. This may be related to ion concentration and other physicochemical properties of roots and root-soil interface.Non-mycorrhizal rootHebeloma sp.Suillus luteus100101102103104105012345678 x 10−4Frequency (Hz)Re (a.u.)  (A)10010110210310410500.0020.0040.0060.0080.0100.0120.0140.016Frequency (Hz)−Im (a.u.)  (B)Non-mycorrhizalHebelomaSuillus

Analysis of mycorrhizal colonisation of Scots pine roots by electrical impedance spectroscopy

https://jukuri.luke.fi/bitstream/10024/542618/1/2013_Repo%20et%20al_%20...._CLAFIC%20analysis%20of%20mycorrhizal%20and%20non-mycorrhizal%20Scots%20pine.pdf

Analysis of mycorrhizal colonisation of Scots pine roots by electrical impedance spectroscopy

Abstract

Similar works

Full text

Available Versions

Jukuri