Becsei, Ágnes

Benkő, Szilvia

Budai, Marietta Margit

Csernoch, László

Danis, Judit

Tőzsér, József

Varga, Alíz

Frontiers in Immunology

University of Debrecen Electronic Archive

Event Abstract  
Back to Event  
Molecular differences of NLRP3 
inflammasome activation in LPS-activated 
human monocyte-derived macrophage 
subtypes 
Marietta M. Budai1, Judit Danis1, Ágnes Becsei1, Aliz Varga1, László Csernoch1, József 
Tőzsér2 and Szilvia Benkő1*  
• 
1
 University of Debrecen, Medical and Health Science Center, Faculty of Medicine, 
Department of Physiology, Hungary  
• 
2
 University of Debrecen, , Medical and Health Science Center, Faculty of Medicine, 
Department of Biochemistry and Molecular Biology, Department of Biochemistry and 
Molecular Biology, Hungary  
IL-1beta is a “master” cytokine that has indispensable roles in orchestrating effective innate 
and adaptive immune responses.  
It is produced in an inactive precursor form that is cleaved to active cytokine  
by protein complexes called Nlrp3 inflammasomes (1). While our knowledge on the  
general mechanisms involved in Nlrp3 inflammasome function and on its regulation  
is rapidly increasing, it is also getting clear that the actual outcome of the  
activation (like IL-1beta production) strongly depends on the cell type and on  
the presence or absence of various intracellular or extracellular modulators (2). Depending  
on their localization macrophages can develop into a wide range of phenotypes. Macrophages  
differentiated in the presence of GM-CSF (GM-MFs) develop inflammatory  
phenotype, while cells differentiated in the presence of M-CSF (M-MFs) possess  
anti-inflammatory characteristics and function in wound healing and tissue  
repair.  
Our results show that following LPS treatment GM-MFs produce high IL-1beta, while M-
MFs produce  
low IL-1beta with a different time- and concentration kinetics. We found  
significant differences in basal and LPS-induced expression of Nlrp3,  
procaspase-1 and ASC between the two MF types. We found that LPS-treated GM-MFs  
are able to release ATP and produce IL-1beta, while M-MFs require ATP  
supplementation for IL-1beta secretion. We have also found expression differences  
in the proteins responsible for ATP release, recognition and degradation as  
well as in the activation of key signal transduction pathways. Furthermore, we  
will show that different nucleosides have strong and differential regulatory  
effects of NLRP3 inflammasome function.  
Acknowledgements 
This work was supported by TÁMOP 4.2.2.A-11/1/KONV-2012-0023 "VÉD-ELEM" grant 
and UD Faculty of Medicine Research Fund - Bridging Fund.  
References 
1. Varga A, Budai MM, Milesz S, Bácsi A, Tőzsér J, Benkő S. 1. Ragweed pollen extract 
intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human 
macrophages. Immunology. (2013) 138(4):392-401. doi: 10.1111/imm.12052.  
 
2. He Y, Franchi L, Núñez G. : 2.TLR agonists stimulate Nlrp3-dependent IL-1β production 
independently of the purinergic P2X7 receptor in dendritic cells and in vivo. J Immunol 
(2013) 190(1):334-9. doi: 10.4049/jimmunol.1202737  
Keywords: Inflammasome, macrophage, IL-1beta, Signal Transduction, P2X7 receptor, ATP, 
Cytokines  
Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 
2013.  
Presentation Type: Abstract 
Topic: Immune receptors and signaling  
Citation: Budai MM, Danis J, Becsei Á, Varga A, Csernoch L, Tőzsér J and Benkő S (2013). 
Molecular differences of NLRP3 inflammasome activation in LPS-activated human 
monocyte-derived macrophage subtypes. Front. Immunol. Conference Abstract: 15th 
International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00471 
Received: 16 Apr 2013; Published Online: 22 Aug 2013.  
* Correspondence: Dr. Szilvia Benkő, University of Debrecen, Medical and Health Science 
Center, Faculty of Medicine, Department of Physiology, Debrecen, H-4012, Hungary, 
szbenko@gmail.com 
 


Molecular differences of NLRP3 inflammasome activation in LPS-activated human monocyte-derived macrophage subtypes

https://dea.lib.unideb.hu/dea/bitstream/2437/221124/1/file_up_10.3389_conf.fimmu.2013.02.00471_absztrakt.pdf

Molecular differences of NLRP3 inflammasome activation in LPS-activated human monocyte-derived macrophage subtypes

Abstract

Similar works

Full text

Available Versions

University of Debrecen Electronic Archive