Béke, Gabriella

Bíró, Tamás

Dajnoki, Zsolt

Gáspár, Krisztián

Hajdú, Klára

Kapitány, Anikó

Kovács, I.

Mócsai, Gábor

Szegedi, Andrea

Töröcsik, Dániel

Journal of Investigative Dermatology

University of Debrecen Electronic Archive

IFNg/IL-17 cytokine milieu is characteristic of papulopustular rosacea 
Gabriella Béke1, 2, Zsolt Dajnoki1, 2, Gábor Mócsai1, 2, Anikó Kapitány1, 2, Krisztián Gáspár1, 2, 
Krisztina Hajdu
1, 2, Dániel Törőcsik1, Ilona Kovács3, Tamás Bíró4, Andrea Szegedi1, 2 
1
Department of Dermatology, 
2
Division of Dermatological Allergology, 
3
Department of 
Pathology,
 4 
Departments of Immunology and Physiology, Faculty of Medicine, University of 
Debrecen, Debrecen, Hungary 
Rosacea is a common chronic inflammatory skin condition typically occurring on sebaceous 
gland rich (SGR) skin areas; its pathophysiology is quite complex and poorly understood.  
We sought to investigate and compare the innate and adaptive immune cell components and 
cytokine profile of papulopustular rosacea (PPR) and SGR skin. 10 PPR and 10 healthy SGR 
skin biopsies were gained which were used for immunohistochemistry to detect CD3
+
 / CD4
+
 
T cells, CD11c
+
 dermal myeloid DCs, CD1a
+
 Langerhans cells, CD163
+
 / Factor XIII
+
 
macrophages. Immunostaining of cytokines mainly but not exclusively characteristic of T 
cells, namely IL-10, IL-13, IL-17 and IFNg was also carried out. May-Grünwald-Giemsa 
(MGG) routine staining was also performed to compare the number of eosinophils, 
neutrophils and mast cells. Cell counts were quantified by Pannoramic Viewer software. 
Biopsies were also used to measure IL-10, IL-13, IL-17 and IFNg gene expression by qPCR. 
In PPR samples infiltrating DCs, T cells and macrophages were detected in significantly 
higher numbers compared to SGR skin. The number of Langerhans cells showed no 
difference. MGG staining revealed that several neutrophils were present in PPR samples, but 
similar to SGR skin eosinophils were missing. Cell count of mast cells was elevated compared 
to SGR skin. Cytokine milieu of PPR skin represented significantly elevated number of IL-
10
+
 and IL-17
+
 cells compared to SGR skin samples and strong IFNg positivity also turned 
up. The gene expression pattern of the cytokines corresponded to our results on protein levels. 
In conclusion inflammatory IFNg/IL-17 cytokine milieu could be a crucial factor in the 
pathogenesis of PPR.  
 


IFNg/IL-17 cytokine milieu is characteristic of papulopustular rosacea

https://dea.lib.unideb.hu/dea/bitstream/handle/2437/216741/ESDR_2015_abstract_NB_Ros_BG.pdf?sequence=4&isAllowed=y

IFNg/IL-17 cytokine milieu is characteristic of papulopustular rosacea

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