Celli, S

Demetris, AJ

Fung, JJ

Kelly, RH

Pan, F

Starzl, TE

Tsugita, M

Valdivia, LA

PubMed

D-Scholarship@Pitt

Metabolic Changes in the Recipient After Successful Liver Xenotransplantation in the Rat S. Celli, L.A. Valdivia, J.J. Fung, R.H. Kelly, F. Pan, M. Tsugita, A.J. Demetris, and T.E. Starzl MOST hepatic functions are genetically determined and. after liver transplantation. the hepatic paren-chyma retains its species specificity. Therefore. in the hamster-to-rat liver xenotransplantation mode!.' we as-sessed the phenotypic changes of the serum protein profile in the recipient. as well as the functional compatibility between the xenoproteins and the recipient environment. Upon serum protein electrophoresis from a normal rat. a xenotransplanted (OL TX) rat, and a hamster. it was evi-dent that the protein profile of the OL TX rat was closer to the hamster than to the rat. In particular. the rat albumin migrated less than hamster albumin. and an OL TX rat at 180 days after transplantation clearly showed hamster albumin. Six days after transplantation OL TX rat serum samples contained albumin with mixed migration charac-teristics. Combining electrophoresis and immunofixation tech-niques we demonstrated the band of migration of comple-ment in the area of the f32-globulins. The OL TX showed a complement band with the same characteristics of the hamster. confirming that in the transplanted rat the main source of complement was of hamster liver origin. By double immunodiffusion assay. using antibodies against rat IgG. it was demonstrated that the IgG in the transplanted rat remained recipient-type. To study the interaction between the hamster complement present in the OL TX serum and the rat IgG, we performed a hemo-lytic assay. In that test, the OL TX complement was able to lyse SRBC with rat antibodies. with the same efficiency as normal rat complement. Another possible functional interaction between donor and recipient proteins was studied on the OLTX bile. The IgA, that have recipient origin because it is produced by the lymphoid tissue. has to be transported into the bile Transplantation Proceedings, Vol 26, No 3 (June), 1994: p 1207 by the secretory component (SC). a membrane protein produced by the biliary epithelium and so of donor origin. With radial immunodiffusion performed in agar containing goat antirat IgA. we tested rat. hamster, and OL TX rat bile and demonstrated the presence of the same amount of rat IgA in the bile of a normal rat and in the bile of an 0 L TX rat. Immunohistochemical analysis of a hamster liver graft stained for IgA showed a distribution of IgA along the perisinusoidal space. and a granular pattern of staining in the cytoplasm. It seemed to suggest preferential binding to receptors (SC) for these immunoglobulins. In conclusion: (I) after liver xenotransplantation. he-patic serum proteins show donor phenotype: (2) molecular chimerism does not necessarily preclude normal protein function: and (3) donor proteins seem able to functionally interact with the recipient environment. So far, no incompatibilities with a normal life have been identified after liver transplantation in the hamster-to-rat model. REFERENCE I. Valdivia LA. Demetris AJ, Fung JJ: Transplantation 55:659, 1993 From the Pittsburgh Transplant Institute, Departments of Sur-gery (S.C., L.A.V., J.J.F., R.HK, F.P., M.T., T.E.S.) and Pathology (A.J.D.), University of Pittsburgh Health Science Center, Pitts-burgh, Pennsylvania. Address reprint requests to Thomas E. Starzl, MD, PhD, De-partment of Surgery, University of Pittsburgh, 3601 Fifth Avenue, 5C Falk Clinic, Pittsburgh, PA 15213. © 1994 by Appleton & Lange 0041-1345/94/$3.001 + 0 1207 

Metabolic changes in the recipient after successful liver xenotransplantation in the rat

Name not available

http://d-scholarship.pitt.edu/5078/1/31735062124999.pdf

Metabolic changes in the recipient after successful liver xenotransplantation in the rat

Abstract

Similar works

Full text

Available Versions

D-Scholarship@Pitt

Name not available

Name not available