Reduction of lymphoid tissue by splenectomy and/or thymectomy has been used as a part of immunosuppression in organ transplantation (4). More recently Walker (7), Johnson (2), Franksson (1) and Starzl (5,6) and their associates have shown that chronic depletion of lymphocytes by thoracic duct drainage decreases the incidence of rejection and hence increases renal graft survival. Mechanical removal of lymphocytes from circulation peripheral blood should theoretically achieve the same or similar effect on the immunity as thoracic duct drainage. Since September, 1979, five organ transplant recipients have received multiple lymphocytapheresis by IBM 2997 Blood Cell Separator as a mechanical pretransplant immunosuppression. The changes in cellular and humoral immunity and the clinical outcome are presented in this report

Iwaki, Y

Iwatsuki, S

Kano, T

Klintmalm, G

Krams, AK

Starzl, TE

D-Scholarship@Pitt

Cytapheresis and Plasma Exchange: Clinical Indications, pages 219-226 © 1982 Alan R. Llss, Inc., 150 Fifth Avenue, New York, NY 10011 LYMPHAPHERESIS IN ORGAN TRANSPLANTATIOH: PRELIMINARY REPORT ABSTRACT S. Iwatsuki, M.D.(l) Y. Iwaki, M.D.(2) G. Klintmalm, M.D.(3) T. Ka no, ~1K D. (3) A-K. Krams, R.N.(3) T. E. Starzl, t1.D. (3) (1) University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania. Work was done at the University of Colorado Medical Center, Denver, Colorado. (2) UCLA School of Hedicine, University of California, Los Angeles, California. (3) Department of Surgery, Denver Veterans Administration Medical Center, the School of Medicine of the University of Colorado Health Sciences Center, Uenver, Colorado. Reduction of lymphoid tissue by splenectomy and/or thymectomy has been used as a part of immunosuppression in organ transplantation (4). More recently Walker (7), Johnson (2), Franksson (1) and Starzl (5,6) and their associates have shown that chronic depletion of lymphocytes by thoracic duct drainage decreases the incidence of rejection and hence increases renal graft survival. Mechanical removal of lymphocytes from circulation peripheral blood should theoretically achieve the same or similar effect on the immunity as thoracic duct drainage. Since September, 1979, five organ transplant recipients have received multiple lymphocytapheresis by fB~1 2997 Blood_Cell Separator as a mechanical pretransplant ir.IfTiunosuppression. The changes in cellular and humoral immunity and the clinical outcome are presented in this report. 220 I Iwatsuki et al r"lATERIAL AND HETHOD Case fviaterial Four patients with end state liver disease and one with end stage renal disease received lymphapheresis three to six times a week prior to transplantation. Sex, age and original disease are listed in Table 1. All four liver patients had massive ascites and, therefore, they were unsuitable for thoracic duct drainage prior to transplantation. One kidney patient had had an unsuccessful thoracic duct drainage before he v,as chosen for lymphocytapheres is. Vascular Access External arterio-venous shunt was created for liver patients using the posterior tibial artery and great saphenous vein at the ankle level. Arterio-venous fistula of the forearm for hemodialysis was used for a kidney patient. Lymphapheresis IBf1 2997 Blood Cell Separator \'Jas used for lymphocytapheresi s. Four thousand to 6,000 units of heparin were given intravenously prior to lymphocytapheresis and 100 to 300 units/min. of heparin were infused regionally throughout the procedure. On some occasions 2-3 ml/min. of ACO-A solution were also infused to prevent the blockage of the WBC line. The blood was processed at the rate of 80 ml/min. and centrifuged at 1150 rpm. Buffy coat was collected at the rate of 2-3 ml/min. through the UBC line. Each lymphocytapheresis lasted for 60 to 120 minutes, processing 5,UOO to 11,UOU ml of blood. Inmunological Monitoring Peripheral lymphocytes were counted before and after each apheresis and the total number of leukocytes and lympho-cytes removed by pheresis were measured each time. Peripheral lymphocyte activity was studied by PHA stimulation and also humoral immunity by ADCC by the method described by Sasaki et a 1 (3). Lymphapheresis in Organ Transplantation I 221 TABLE 1. Age, sex and Original Disease of Five Patients Who Received Long Term Lymphocytapheresis Case Age Sex Origi nal Disease qrans~lant 1 33 ~lal e Primary biliary cirrhosis Liver 2 32 Female Chronic active hepatitis liver 3 27 Female Chronic active hepatitis Liver 4 36 Female Secondary biliary cirrhosis Liver 5 42 ~lale End-stage renal disease (?CGN) Renal RESULTS A. Lymphocytapheresis Each procedure lasted 60 to 120 minutes and 4,000 to 1U,OUU m1 of blood was processed each time. Sixteen to 42 aphereses were performed during the period of 21 to 52 days. The total number of lymphocytes removed by a series of aphereses prior to transplant ranged from 14.0 x 10 9 to 103.7 x 109, IISC (including lymphocytes) ranged from J.8.2 x 10 9 to 376 x 109, and lymphocyte-rich plasma from 3629 to 9839 ml in five cases (Table 2). TABLE 2. Total Number of Lymphocytes, WBC and Plasma Removed by Lymphocytapheresis Period riumber of Lymphocytes \JBC Plasma of Apheresis A~heresis {aa~s~ {x10 9 ~ {x1M9~ {ml ~ Case 1. 22 30 69.1 83.6 3629 Case 2. 21 35 25.5 39.0 4192 Case 3. 16 21 14.0 18.2 4424 Case 4. 35 52 103.7 376.0 7514 Case 5. 42 52 93.5 146.2 9839 222 I Iwatsuki et al The mean number of lymphocytes, WBC, and platelets removed by a single lymphocytapheresis are summarized in Table 3; lymphocytes ranged from 0.9 x 10 9 to 3.5 X 10 9, WBC from 1.1 x 109 to 10.7 X 109, platelets from 10.5 x 10 6 to 122.6 X 109 and lymphocyte-rich plasma 189 to 275 ml. TABLE 3. Mean Number of Lymphocytes, WBC, Platelets and Plasma Removed by Each Lymphocytapheresis Lymphocytes WBC Platelet Plasma (XI09} (xl09} {x10 6 } (011 } Case 1. 3.5 ± 2.0 4.5 ± 2.6 144.0 ± 14.3 189 ± 46 Case 2. 1.3 ± 0.7 2.0 ± 1.2 83.7 ± 11.3 196 ± 32 Case 3. 0.9 ± 0.4 1.1 ± 0.4 10.5 ± 1.7 275 ± 45 Case 4. 2.9 ± 3.6 10.7 ± 15.1 78.2 ± 10.0 208 ± 40 Case 5. 2.1 ± 1.5 3.5 ± 2.0 122.6 ± 9.9 239 ± 39 The mean changes in peripheral blood, WBC, lymphocytes, platelet count and hematocrit by a single lymphocytapheresis are summarized in Table 4; ~gBC and lymphocyte count decreased significantly in three of five cases (p < 0.05); the platelet count decreased in all five cases, and the hematocrit in four of five cases. TABLE 4 Mean Changes in Peripheral Blood WBC, Lymphocytes, Platelet Count and Hematocrit Before and After Lymphocytapheresis WBC Lymphocytes Platelet {Lmm 3 } {Lmm3 } (xl03/mm 3} Hematocrit {%} Case 1. -730(-10%} -180(-7%} -16(-10%} -3.2(-12%} p<0.1 n.s p<O.Ol p<O.OOI Case 2. -2500(-17%} -300(-37%) -33(-26%} -3.8(-17%} p< O. 01 p<0.05 p<O.OOl p<O.OOl Case 3. -500(-12%) -100(-14%) -13(-21%) -3.6(-13%} n.s p<0.1 p<O.OOl n.s Case 4. -2400(-16%) -500(-45%) -65(-13%} -3.4(-13%) p< 0.001 p<0.001 p<O.OOl p<O.OOl Case 5. -600(-7%} -200(-15%) -87(-35%) -2.8{-12%} p< 0.05 p<0.01 p<0.001 p<0.001 Lymphapheresis in Organ Transplantation I 223 By a series of aphereses, peripheral blood WBC count decreased significantly in one of five cases (Case 3). The lymphocyte count decreased in four of five cases (Cases 2, 3, 4, and 5). The platelet count decreased slightly in all of the five cases, and the hematocrit decreased also in all cases. In four of five cases, blood transfusion was necessary to improve the general condition of the patients. B. Immunological Changes Cellular immunity measured by PHA stimulation index (3) decreased significantly by a series of lymphocytapheresis in three of five cases (Cases 1, 2, and 4). Humoral immunity monitored by ADCC (3) decreased in three of five cases (Cases 2, 3, and 4). A strong antimitochondrial antibody present in Case 1 completely disappeared after a series of lymphocytaphereses. C. Results of Transplant THO of the four liver transplant recipients are alive and well (Cases 3 and 4), without any evidence of graft rejection. One liver recipient (Case 1) died after 10 days because of a poorly functioning graft and another recipient died of pulmonary sepsis after three weeks. One kidney recipient is doing y~ell with normal renal function in the third post-transplantation month. Figures 1 and 2 illustrate the changes in peripheral blood counts and immunity by a series of lymphocytaphereses. DISCUSSION The number of lymphocytes removed by lymphocytapheresis largely depends upon the lymphocyte count and the hematocrit of pheripheral blood. Peripheral blood lymphocyte count of less than RMM/~1 in the presence of hypersplenism and the hematocrit of less than 20% make the yield of lymphocytapheresis minimal. The clumping of leukocytes and platelets in the centrifuge and in the tubing system can occasionally become a problem with heparin anticoagulation. Additional ACD-A anticoagulant does not always solve this problem. Improvement 224 I Iwatsuki et al PHA STIMULATION INDEX ADCC PERCENT CYTOLYSIS AMA HEMATOCRIT 1%) PERIPHERAL BLOOD PLATLET COUNT (/mm3 ) PERIPHERAL BLOOD LYMPHOCYTE COUNT (lmm3) PERIPHERAL BLOOD LEUKOCYTE COUNT Vmm 3) CUMULATIVE LYMPHOCYTES REMOVED BY PHERESIS 40 ~~~-30 20 ~ ~ 10 -  ~ 0+----------40 PM~ ORMDl~~l~ 200,000 _____ _ 150,000 "-J -~ 100,000_ 2,500 2,000 1,500 1,000 500 o 15,000 1MDlll~ "~~M~~1·---·------:~ ~ 20 ~ l~rl =rI-~I -Df~~f-~f~f I 5 10 15 20 25 30 TIME IN DAYS Figure 1. Case 1 is a 33-year-old male with primary biliary cirrhosis. The PHA stimulation index decreased rapidly by a series of lymphocytaphereses. Antimitochondrial antibody (ANA) disappeared after a three-week course of lymphocytapheresis. of the tubing material, such as the Teflon coating of tubes and filters, may be helpful. Heparin seems to be better tolerated by the cirrhotics and the uremics than citrate anticoagulation. Fluid balance is very important for these patients, as fluid overload with priming saline to minimize the red cells and protein loss should be avoided. Cellular immunity monitored by PHA stimulation and/or humoral immunity by ADCC decreased in four of five cases. These immunological alterations correlate with the numbers of lymphocytes removed by lymphocytapheresis on an individual basis. However, adequate immunosuppression was achieved by a relatively small amount of lymphocytapheresis in Cases 2 and 3. No effect was observed in Case 5 in spite of a large amount of lymphocytapheresis. A decrease in peripheral blood lymphocyte count did not always correlate with immunological suppression monitored Hith PHA stimulation and ADCC. PHA STIMULATION INDEX Aoec PERCENT CYTOLYSIS HEMATOCRIT (-.to) PERIPHERAL BLOOD PLATLET COUNT itmm3) 40 '0 20 10 o 40 '0 20 Lymphapheresis in Organ Transplantation I 225 D~ ---- -=-=--=========:=:-: 40 tfUNITS 2+L.lNITS '0 20 ~ooKo~~r-- ----------200,000 100.000,__ ____ __ 4,000 PERIPHERAL BLOOD 3.000 LYMPHOCYTE COUNT (1m",') 2.000 PERIPHERAL BLOOD LEUKOCYTE COUNT (1m"," 1,000 o 2:0.000 10,000 Figure 2. Case 4 is that of a 36-year-old female with secondary biliary cirrhosis. The PHA stimulation index and AOCC (% cystolysis) decreased rapidly by lymphocytapheresis. ( i- indicates blood transfusion). Our limited clinical experience and primitive immuno-logical studies in the five cases seem to indicate that lymphocytapheresis decreases cellular and humoral immunity and prevents rejection in organ transplant recipients. Detailed immunological analyses are being undertaken at present. The disappearance of the antimitochondrial antibodies in Case 1 during lymphocytaphereses needs to be confirmed. Additional patients with primary biliary cirrhosis are now planned for lymphocytapheresis. The clinical significance of removing the antimitochondrial antibodies in these patients can only be discussed when more substantial data are available. ACKNOWLEDGNENT Supported by research projects from the Veterans Admin-istration; by United States Public Health Service Grants AN-17260 and AM-07772; and by Grants RR-00051 and RR-00069 226 I Iwatsuki et al from the General Clinical Research Centers Program of the Uivision of Research Resources. National Institutes of Health. REFERENCES 1. Franksson C, Lundgren G. Magnusson G. Ringden 0 (1976). Drainage of thoracic duct lymph in renal transplant patients. Transplantation 21:133. 2. Johnson HK. Niblack GD. Tallent MB. Richie RE (1977). Immunologic preparation for cadaver renal transplant by thoracic duct drainage. Transplant Proc 9:1499. 3. Sasaki M, Terasaki PI, Silberman H. Berne T (1979). Antibody-dependent cell-mediated cytotoxicity tests for immunosuppression monitoring of transplant patients. Transplantation 27:246. 4. Starzl TE (1964). "Experience in Renal Transplantation." Philadelphia: W. B. Saunders Co., p 126. 5. Starzl TE, Weil RIll, Koep LJ, McCalmon RT Jr. Terasaki PI, Iwaki Y, Schroter GPJ, Franks JJ, Subryan V, Halgrimson CG (1979). Thoracic duct drainage and renal transplantation. Ann Surg 190:474. 6. Starzl TE, Weil RIll, Koep LJ. Iwaki Y, Terasaki PI, Schroter GPJ (1979). Thoracic duct drainage before and after cadaveric kidney transplantation. Surg ~ynecol Obstet 149:815. 7. Walker WE, Niblack GO. Richie RE. Johnson HK, Tallent MB (1977). Use of thoracic duct drainage in human renal transplantation. Surg Forum 28:316. 

Lymphapheresis in organ transplantation: preliminary report.

Krams, A-K

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Lymphapheresis in organ transplantation: Preliminary report

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Lymphapheresis in organ transplantation: preliminary report.

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