Habermann, J.

Henner, J.

Horn, K.

Horst, I. zur

Scriba, Peter Christian

English

Open Access LMU

Acta endocrinologica Supplementum 159 A D V A N C E A B S T R A C T S OF SHORT PAPERS 18. Symposion Deutsche Gesellschaft für Endokrinologie Unter Mitwirkung der Deutschen Gesellschaft für Klinische Chemie Hannover March 1-4, 1972 P E R I O D I C A - C O P E N H A G E N 1972 C O N T E N T S TRH, TSH, Schilddrüse LCR. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime and P. C. Scriba: TRF-stimulation-test in hypothalamic and pituitary diseases, and in autonomous adenoma of the thyroid. 2. G. Rothenbuchner, J. Birk, U. Loos, S. Raptis and £. F. Pfeiffer: The dynamics of TSH-secretion after oral administration of synthetic thyro-tropin-releasing factor (TRF) in metabolically normal subjects. 3. G. Rothenbuchner, U. Loos, G. Knapp, I. Birk, W. R. Kießling and E. F. Pfeiffer: The alterations of total-thyroxine and total-triiodothyronine after oral application of synthetic thyrotropin-releasing factor (TRF) in metabolically normal subjects. 4. K. Retiene, H. Holz, A. Müller, R. Guthoff, H.-J. Ewers, K. M. Bartelt, P. Althoff and V. Grabs: Further experimental und clinical findings during long-term administration of synthetic TRH. 5. F. Adlkofer, H. Schleusener and A. von zur Mühlen: THS activity in extracts from hyperthyroid plasma before and after administration of thyrotropin-releasing factor (TRH). 6. R.-D. Hesch, M. Hüfner and A. v. z. Mühlen: Radioimmunoassay of triiodothyronine in thyroid diseases. 7. / . Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba: Auto-matization of serum thyroxine determination and of T3-uptake test. 8. K, Horn, H. Borowzak, /. Habermann, M. Rettig, T. Ruhl and P. C. Scriba: Total serum Ta and T 4 in nontoxic goiter and autonomous adenoma. 9. K, Hackenberg, R. Schneider and D. Reinwein: Thyroidal suppressibility and thyroglobulin antibodies during treatment of thyrotoxicosis with anti-thyroid drugs. 10. F. W. Lohmann, H. Schleusener and F. Adlkofer: The sympathoadrenal system in hyperthyroidism. 11. / . Nolte, D. Brdiczka, P. C. Scriba und H. W. Staudte: Schilddrüsen-hormonwirkung im Riboflavinmangel. 12. K. Schimmelpfennig, A. Kaul and E. Köhler: Problems connected with in-vestigations of athyroid experimental animals. SteroidstoffWechsel und -Wirkungen 13. W. Mestwerdt, H. Brandau und H. H. Kley: Struktur und Funktion steroid-aktiver Zellen im Postmenopauseovar. 14. M. Breckwoldt, W. Wortmann, J. C. Touchstone and B. P. Lisboa: In-vivo perfusion of the kidney with 3H-estradiol-17/? in the rhesus monkey. V 15. P. Benes and G. W. Oertel: Penetration of intact mitochondria by dehydroepiandrosterone, its conjugates or derivatives. 16. H. Brandau und G. Mutzke: Aktivitätsmuster von Hydroxy Steroid-Dehy-drogenasen im Corpus luteum des Rindes. 17. Jeanne Sioe Eng Dericks-Tan: Metabolization of ethinyl-nor-testosterone acetate by female rat liver microsomes. 18. D. Engelhardt, P. Unterburger and H. J. Karl: Studies of Ci9-steroid-enzyme activities in normal and pathological human liver tissue. 19. R. Ghraf, E. R. Lax, H. Ockenfels and H. Schrie fers: Sex- and age-dependent hydroxylations of testosterone in the liver of normally developed rats and in animals with disturbed sexual development. 20. B. P. Lisboa and J.-C. Plasse: zl5-3a-hydroxysteroid-dehydrogenase activity in the human fetal liver. 21. H. Becker, E. Grabosch and K. Voigt: Metabolism and mode of action of androgens in target tissues of male rats 1. Metabolism of 4a-androstan-3ß, 17/?-diol in target organs and in peripheral tissues. 22. H. Schmidt, G. v. Rotteck and K. D. Voigt: Metabolism and mode of action of androgens in target tissues of male rats 2. Mode of action of 5a-androstan-3/?, 17/?-diol in target organs and peripheral tissues. 23. H-G. Hoff, R. Ghraf and H. Schriefers: Androgen-dependency of the sex-specific differentiation of the activity of enzymes involved in steroid metabolism. 24. G. Trams, P. Br Ummer and H. Maass: Studies on the interrelationship be-tween oestrogens and gestagens in the target tissue. 25. Ch. Seyfried and H-G. Kraft: Nucleic acid metabolism in the rat uterus: effect of EMD 16.795, a competitive anti-oestrogen. Renin, Angiotensin 26. W. Vogt, Kh. R. Koczorek, H.-H. Vogt und M. Knedel: Polypeptid-hormonnachweis durch Kupplung mit Diazoniumsalzen. 27. A. Philippi, S. C. Lowder, F. Lamprecht and F. Gross: Effects of posture and exercise on the urinary clearance of renin in man. 28. H. P. Nast und A. Distler: Untersuchungen über den Abbau von Angiotensin in menschlichen Erythrozyten. 29. H. C. Siemensen: Untersuchungen an der Ratte zur Bedeutung der Leber für die Renin-Angiotensin-Kinetik. 30. H. Wernze, A. Seki and R. Jesse: Hepatic renin extraction in hypertension and cirrhosis of the liver. 31. R. Beckerhoff, J. A. Luetscher and W. Siegenthaler: Changes in renin-angiotensin system induced by oral contraceptives. Aldosteron 32. W. Vetter, E. Freedlender and E. Haber: Production and characterization of antibodies elicited by different aldosterone-protein conjugates. 33. P. Vecsei, C. Akangbou, A. Joumaah and N. I. Salloum: Studies on anti-bodies against corticoid hormones. VI 34. W. R. Külpmann, L . Siekmann and H. Breuer: An improved gas chromato-graphic method for the determination of aldosterone in urine. 35. K. A. Deck, P. K. Champion, D. R. Rovner and / . W. Conn: Urinary free aldosterone in normal persons and in patients with primary aldosteronism. 36. D. Lommer, A. Distler, T. Philipp and H. P. Wolff: Secretion, distribution and turnover of aldosterone in essential hypertension, primary aldosteronism, and hypertension associated with renal artery stenosis. Prolaktin 37. M. UHermite, V. Stavric and C. Robyn: Human pituitary prolactin during pregnancy and post partum as measured in serum by a radioimmunoassay. 38. P. Berle und K-D. Voigt: Plasma-Prolaktinkonzentrationen beim Mamma-karzinom der Frau. 39. D. Schams, V. Reinhardt and H. Karg: Effects of a synthetic ergot alkaloid on the peripheral blood level of prolactin in cows. 40. R. Wenner und L . Varga: Prolactinhemmung mittels Ergocryptin. 41. M. Little: The effect of prolactin and growth hormone preparations on the casein production in mouse mammary gland tissue explants. 42. U. Hachmeister, R. Fahlbusch and K. v. Werder: Ultrastructural identity of pituitary adenoma cells in Forbes-Albright syndrome and of adenohypo-physeal pregnancy cells. Wachstumshormon 43. K.v. Werder, M. Gottsmann, A. Souvatzoglou, P. Botterman and K. Schwarz: Growth hormone and growth hormone-like immunoreactivity in man. 44. P. H. Althoff, B. Schneider, K. M. Bartelt, K. Retiene und K. Schaffung: Der Glucagon-Stimulationstest, eine Methode zur Klassifizierung der Akro-megalien — eine primäre, hypophysäre und eine sekundäre, hypothalamische Form? 45. A. von zur Mühlen, J. Köbberling and A. Bachmann: The effect of repeated depot A C T H injections on arginine-induced H G H release in healthy subjects and patients with Addison's disease. 46. W. Wiegelmann, H. G. Solbach, H. Bethge and H. Zimmermann: Growth hormone response to insulin-induced hypoglycaemia in anorexia nervosa. 47. H. Nowakowski, R. Kautzky, N. Stahnke and B. Regler: Selective removal of pituitary adenomas in acromegaly. 48. K. Hübner, K. H. Usadel and R. Schneider: Histoautoradiographic investi-gation on the influence of hypophysectomy and STH-substitution on the regeneration of renal tubular epithelium following temporary ischemia. Adiuretin 49. H. D. Freisenhausen and H. Frahm: Development of a radioimmunoassay for vasopressin. 50. H. U.Tietze, A. Chattas and O. Oetliker: Behavior of free water clearance in children with vasopressin-sensitive diabetes insipidus and in children with renal diabetes insipidus under treatment with carbamyl-dibenzo-azepine (Tegretal). VI I 51. F. Ublieb, K. Loeschke and / . Eigler: Antidiuretic effect of Carbamazepin in central diabetes insipidus. 52. K. Irmscher, W. Wiegelmann, H. Bethge, J. Kampf jmeier, H. G. Solbach and H. Zimmermann: Therapeutic response of central diabetes insipidus on nasal application of a new synthetic analogue of vasopressin (l-deamino-8-d-arginine-vasopressin (DDAVP). Gonadotropin-Releasing-Faktoren 53. F. Enzmann, K. Geisen, R. Geiger, W. König and H. Wissmann: Isolation and characterization of two different porcine hypothalamic preparations with LH-RH-like activity and their comparison with synthetic LH-RH. 54. / . Sandow, A. V. Schally, H. G. Schröder, W. Heptner and F. Enzmann: FSH-releasing activity of a synthetic decapeptide (LH/FSH-RH). 55. S. Heller, H. D. Schlumberger, F. Kümmel and U. Maier: FSH-release by L-3-O-methyl-dopa and L-dopa. 56. H. G. Schröder, K. Engelbart, J. Sandow and W. Heptner: The effect of synthetic LH/FSH-RH on the maturation of infantile female rats. 57. A. von zur Mühlen and / . Köbberling: The effect of synthetic luteinizing hormone releasing factor (LH-RF) on the release of hormones of the adenohypophysis in normal men. 58. K. Seeger, /. Sandow, H. G. Schröder and W. Heptner: Effect of synthetic LH/FSH-RH on LH-release and ovarian function in sheep. 59. H. Kühl, W. Bichel, /. Enenkel, G. Hauptmann, P. Chandra and H.-D. Taubert: Contrasting effects of some steroidal hormones upon L-cystine-aminopeptidase activitiy in the hypothalamus of immature male und female rats. 60. A. Souvatzoglou, P. Bottermann, K. v. Werder and K. Schwarz: Effect of L-dopa on L H and G H levels in man. 61. F. Lehmann, F. Peters, M. Breckwoldt and G. Bettendorf: Plasmaprogestins during infusion of prostaglandin F20C Calcitonin, Parathormon 62. F. Gniffke, P. O. Schwüle and H. Bünte: Ionized calcium by selective electrode assay, normal range, loading tests. 63. K. Paschen, C. Fuchs and R-D. Hesch: A simple and precise method for determination of ionized calcium in serum by an ionselective electrode. 64. R. Hehrmann, J. Hagemann and R. Montz: Studies on endocrine regulations of intestinal calcium absorption. 65. M. Hüfner, R.-D. Hesch, H. Schmidt, M. Hasenjäger, K. Winckler, W. Creutzfeldt and K. Paschen: The gastrointestinal effects of calcitonin. 66. C. Lozano-Tonkin, H.-P. Kruse und H.-R. Montz: Auswertung klinischer und mikroradiographischer Befunde beim Hyperparathyreoidismus sowie ihre Korrelation zu Ergebnissen der 4 7Calcium-Kinetik. 67. R. Montz, R. Hehrmann, F. Kuhlencordt and C. Schneider: 4 7Calcium ki-netics in the evaluation of parathyroid function. VI I I 68. E. Altenähr, R. Hehrmann and H-P. Kruse: Correlation of ultrastructural criteria of human parathyroid gland function with calcium and bone metabolism in hyperparathyroidism. 69. G. Delling: Effect of calcitonin on increased bone resorption and osteoporotic changes induced by chronic acid feeding in the rat. 70. H. van Lessen, G. Rodeck and H. Bechtelsheimer: Medullary carcinoma of the thyroid and hyperparathyroidism. 71. F. Raue, H. Minne, S. Bellwinkel and R. Ziegler: Studies on the hypercalcemic syndrome in rats with Walker carcinosarcoma 256. HVL-NNR-System 72. K. H. Voigt, H. L . Fehm and E. F. Pfeiffer: Extraction of ACTH from plasma for use in radioimmunoassay. 73. H. L . Fehm, K. H. Voigt and £. F. Pfeiffer: Spontaneous and stimulated secretion of immunoreactive A C T H in man. 74. / . Homoki and W. Teller: Studies on cortisol/corticosterone ratio in plasma as indirect measure of 17a-hydroxylase activity during childhood. 75. W. Winkelmann, D. Hessen and R. Mies: 11-desoxycortisol and Cortisol pro-duction in congenital adrenocortical syndrome. 76. G. Zieger: Features of growth in the capsular area of the human adrenal cortex. 77. K. J. Beck, G. Leyendecker and W. Nocke: Comparison of oestrogen con-centration in serum and cervical mucus. 78. Ch. Lauritzen and W. D. Lehmann: The feto-placental D H A load test: bases, results, interpretation. 79. K. Wenzel, W. Obolensky and P. Vulliemoz: DHEAS-test and nutritional insufficieny of the placenta. 80. W. Obolensky and K. Wenzel: Changes in estriol concentration in amniotic fluid after DHEAS-loading. 81. W. Geiger and W. Post: The distribution of HCG, HCS, STH and TSH to various physiological fluids of mother and fetus in the last trimester of pregnancy. 82. B. Hoffmann, D. Schams and H. Karg: Simultaneous determination of progesterone, total oestrogens, corticoids, prolactin and L H in peripheral blood of the cow around parturition. Katecholamine 83. R. Gugler, ] . H, Hengstmann and H. J. Dengler: Clinical and biochemical findings in a malignant pheochromocytoma treated with alpha-methyl-p-tyrosine. 84. W. Gusek und M. Fock: Ultrastruktur hormondifferenter Phäochromo-zytome. 85. P. Ball, R. Knuppen, W. Wennrich and H. Breuer: Interactions between oestrogens and catecholamines: influence of oestrogens on the effect of catecholamines on blood pressure in rats. 86. F. W. Schmahl, F. Buchholz, K. Huth and B. Urbaschek: Effects of norepinephrine and orciprenaline on endotoxin-induced alterations of blood lipids. I X Diabetes mellitus 87. H. Schatz, Y. Abdel Rahman, M. Hinz, V. Maier, C. Nierle and E. F. Pfeiffer: The effect of growth hormone (GH) and adrenocorticotrophic hormone (ACTH) on secretion and biosynthesis of insulin in isolated islets of the rat. 88. H. Laube, E. Grajeda, R. Fussgänger and E. F. Pfeiffer: Influence of adrenergic receptors on glucose-induced hyperinsulinemia of obese mice. 89. W. Fechner and M. Uage: Development of acute diabetic ketoacidosis and its correlation to plasma corticosterone levels in the alloxanized rat. 90. G. Klöppel, E. Altenähr and G. Freytag: Electronmicroscopic studies on cellular autoimmunity against insulin in the pancreas of rabbits. 91. H. v. Lilienfeld-Toal, M. Hüfner, C. Fuchs and R. D. Hesch: Radio-receptorassay for 3', 5'-AMP. 92. P. O. Schwüle and B. Barth: Clearance and stop flow studies in glucagon mediated hyperelectrolyturia. Androgene, Hirsutismus 93. E. Nieschlag, D. L . Loriaux and M. B. Lipsett: Radioimmunoassay for de-ny droepiandrosterone and its sulfate: effect of ACTH, gonadotropins and dexamethasone on plasma levels. 94. L . Penzes and G. W. Oertel: Estimation of oxosteroids by densitometry of suitable derivatives. 95. W.-D. Hetzel, R. Kiehnscherf und W. Staib: Extraktion von Steroidkonju-gaten durch flüssige Ionenaustauscher. 96. D. Knorr und O. Butenandt: Hodenfunktionstest durch HCG-Stimulation und Plasmatestosteronbestimmung in der Vorpubertät. 97. K.-P. Littmann und H. Gerdes: Testosterontagesrhythmik und HCG-Stimu-lation bei akuten und chronischen Lebererkrankungen. 98. W. M. Teller and H. Ferlemann: The influence of human chorionic gonado-tropin (HCG) on urinary steroid patterns in prepubertal boys with distopic testes. 99. K. H. Usadel, U. Schwedes, C. Schade, U. Leuschner und K. Schöffling: Nervenfasern in den Tubuluswänden der Hoden von Klinefelter-Patienten. Histochemische und elektronenoptische Befunde. 100. P. Göbel, J. Cyran, V. Frick, A. E. Schindler und M. Schwarz: Anti-androgentherapie des Hirsutismus. Gonadotropine 101. D. Graesslin, H. Gh. Weise, W. Braendle and G. Bettendorf: Isolation and partial characterisation of human pituitary FSH and L H . 102. L . Wildt, G. Leyendecker and W. Nocke: Studios on gel filtration of plasma L H and FSH. 103. H. Gh. Weise, D. Graesslin, P.-J. Czygan and W. Braendle: Biological, immunological and physicochemical properties of several different HCG components. 104. W. Braendle, P.-J. Czygan, D. Graesslin and H. Ch. Weise: Distribution and binding of 1 3 1 J-HCG in different organs of the female rat. X 105. W. Geiger: The role of standard preparations and antisera in the differences of "absolute" LH-measurement. 106. W. Heptner, E. Niemann, J. Sandow, N. Seher and F. Enzmann: Studies on the reactivity of fractions of 125 I-labelled ovine L H in radioimmuno-assay and bioassay. 107. H. Hildebrandt, M. Vekemans and C. Robyn: Radioimmunoassays for human chorionic gonadotropin (HCG) and human pituitary luteinizing hormone (HLH) using the double antibody method: comparative evaluation of equilibrium and non-equilibrium conditions. 108. P-J. Czygan: Different feedback action of oestrogens in pre- and post-menopausal women. 109. D. Lindenmeyer and / . Tamm: Plasma levels of L H / H C G in male subjects following intravenous administration of HCG or oral treatment with Clomiphen. 110. D. Schams, H. D. Butz and H. Karg: Sex differences on plasma luteinizing hormone level from birth to puberty in the bovine. X I Acta endocr. (Kbh.) Suppl. 159 (1972) 7 18. Symp. Dtsch. Ges. Endokrin. Abstract No. 7 I I . Medizinische Klinik der Universität München AUTOMATIZATION OF SERUM THYROXINE DETERMINATION A N D OF Ts-UPTAKE TEST* /. Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba Problem: Simple, rapid and inexpensive techniques were developed, since in this labora-tory the requests for determinations of T4 an of T3-uptake exceed the number of 200 per week. This study was aimed at the development and the automatization of a competitive protein binding method for T4 on the basis of the automatic T3-uptake test in use. Methods: 1. Ts-uptake-test: 0.2 ml serum and 0.4 ml T 3 - 1 2 5 J (0.1 ^Ci) in 0.5% albu-min, 0.05 M sodium phosphate buffer p H 7.4 were equilibrated for 15 min at room temperature. 25 samples were simultaneously aspirated by a 25-channel peristaltic pump and delivered to 25 microcolumns containing 1 g sephadex G-25 fine, placed in a thermoblock (29° C) for separation of protein-bound and so-called free = dextran gel adsorbed T 3 - 1 2 5 J . The latter was totally eluted by pooled serum. With this procedure the same columns could be used for weeks. The whole separation was performed automatically within 25 min with a time-controlled apparatus. 2. Serum-Ti: 0.1 ml serum and 0.3 ml T 4 - 1 2 5 J (0.1 ^Ci) in 0.5% albumin, 0.05 M sodium phosphate buffer ph 7.4 are incubated for 15 min at room temperature. The incubation mixtures are delivered as above to 1 g sephadex G-25 s. f. columns, which are alkalized with 0.02 N NaOH, resulting in complete retention of cold endogenous and radioactive T4. The columns are then washed with 0.05 M sodium barbital buffer p H 8.6. Addition of 1.6 ml pregnancy serum (11:00 diluted in barbital buffer) caused competitive protein binding for T4 on the column and simultaneously eluted so bound T4. The relative radioactivity in this eluate indicated the amount of endogenous T4. The remaining hormones were finally totally eluted from the columns with excess serum, so that re-use of the columns was possible. For this CPB-method for serum T4, the same automatic apparatus as for the T3-uptake test is used. Results: The normal range of Ts-uptake (x ± 2 s) is 32—42%; quality control is satisfactory with a variation coefficient of 3.5% for a control serum checked for more than 2 years. Clinical data and quality control of the automatic CPB-method for T4 wi l l be presented. * Supported by the Deutsche Forschungsgemeinschaft (SFB 51). 7 

Automatization of serum thyroxine determination and of T3-uptake test

J. Habermann

J. Henner

K. Horn

I. zur Horst

P. C. Scriba

Crossref

AUTOMATIZATION OF SERUM THYROXINE DETERMINATION AND OF T3-UPTAKE TEST

Universität München: Elektronischen Publikationen

Acta 
endocrinologica 
Supplementum 159 
A D V A N C E A B S T R A C T S 
OF 
SHORT PAPERS 
18. Symposion Deutsche Gesellschaft für Endokrinologie 
Unter Mitwirkung der Deutschen Gesellschaft für Klinische 
Chemie 
Hannover 
March 1-4, 1972 
P E R I O D I C A - C O P E N H A G E N 1972 
C O N T E N T S 
TRH, TSH, Schilddrüse 
LCR. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime and 
P. C. Scriba: TRF-stimulation-test in hypothalamic and pituitary diseases, 
and in autonomous adenoma of the thyroid. 
2. G. Rothenbuchner, J. Birk, U. Loos, S. Raptis and £. F. Pfeiffer: The 
dynamics of TSH-secretion after oral administration of synthetic thyro-
tropin-releasing factor (TRF) in metabolically normal subjects. 
3. G. Rothenbuchner, U. Loos, G. Knapp, I. Birk, W. R. Kießling and E. F. 
Pfeiffer: The alterations of total-thyroxine and total-triiodothyronine 
after oral application of synthetic thyrotropin-releasing factor (TRF) in 
metabolically normal subjects. 
4. K. Retiene, H. Holz, A. Müller, R. Guthoff, H.-J. Ewers, K. M. Bartelt, 
P. Althoff and V. Grabs: Further experimental und clinical findings during 
long-term administration of synthetic TRH. 
5. F. Adlkofer, H. Schleusener and A. von zur Mühlen: THS activity in 
extracts from hyperthyroid plasma before and after administration of 
thyrotropin-releasing factor (TRH). 
6. R.-D. Hesch, M. Hüfner and A. v. z. Mühlen: Radioimmunoassay of 
triiodothyronine in thyroid diseases. 
7. / . Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba: Auto-
matization of serum thyroxine determination and of T3-uptake test. 
8. K, Horn, H. Borowzak, /. Habermann, M. Rettig, T. Ruhl and P. C. Scriba: 
Total serum Ta and T 4 in nontoxic goiter and autonomous adenoma. 
9. K, Hackenberg, R. Schneider and D. Reinwein: Thyroidal suppressibility 
and thyroglobulin antibodies during treatment of thyrotoxicosis with anti-
thyroid drugs. 
10. F. W. Lohmann, H. Schleusener and F. Adlkofer: The sympathoadrenal 
system in hyperthyroidism. 
11. / . Nolte, D. Brdiczka, P. C. Scriba und H. W. Staudte: Schilddrüsen-
hormonwirkung im Riboflavinmangel. 
12. K. Schimmelpfennig, A. Kaul and E. Köhler: Problems connected with in-
vestigations of athyroid experimental animals. 
SteroidstoffWechsel und -Wirkungen 
13. W. Mestwerdt, H. Brandau und H. H. Kley: Struktur und Funktion steroid-
aktiver Zellen im Postmenopauseovar. 
14. M. Breckwoldt, W. Wortmann, J. C. Touchstone and B. P. Lisboa: In-vivo 
perfusion of the kidney with 3H-estradiol-17/? in the rhesus monkey. 
V 
15. P. Benes and G. W. Oertel: Penetration of intact mitochondria by 
dehydroepiandrosterone, its conjugates or derivatives. 
16. H. Brandau und G. Mutzke: Aktivitätsmuster von Hydroxy Steroid-Dehy-
drogenasen im Corpus luteum des Rindes. 
17. Jeanne Sioe Eng Dericks-Tan: Metabolization of ethinyl-nor-testosterone 
acetate by female rat liver microsomes. 
18. D. Engelhardt, P. Unterburger and H. J. Karl: Studies of Ci9-steroid-
enzyme activities in normal and pathological human liver tissue. 
19. R. Ghraf, E. R. Lax, H. Ockenfels and H. Schrie fers: Sex- and age-
dependent hydroxylations of testosterone in the liver of normally developed 
rats and in animals with disturbed sexual development. 
20. B. P. Lisboa and J.-C. Plasse: zl5-3a-hydroxysteroid-dehydrogenase activity 
in the human fetal liver. 
21. H. Becker, E. Grabosch and K. Voigt: Metabolism and mode of action 
of androgens in target tissues of male rats 1. Metabolism of 4a-androstan-
3ß, 17/?-diol in target organs and in peripheral tissues. 
22. H. Schmidt, G. v. Rotteck and K. D. Voigt: Metabolism and mode of action 
of androgens in target tissues of male rats 2. Mode of action of 5a-
androstan-3/?, 17/?-diol in target organs and peripheral tissues. 
23. H-G. Hoff, R. Ghraf and H. Schriefers: Androgen-dependency of the 
sex-specific differentiation of the activity of enzymes involved in steroid 
metabolism. 
24. G. Trams, P. Br Ummer and H. Maass: Studies on the interrelationship be-
tween oestrogens and gestagens in the target tissue. 
25. Ch. Seyfried and H-G. Kraft: Nucleic acid metabolism in the rat uterus: 
effect of EMD 16.795, a competitive anti-oestrogen. 
Renin, Angiotensin 
26. W. Vogt, Kh. R. Koczorek, H.-H. Vogt und M. Knedel: Polypeptid-
hormonnachweis durch Kupplung mit Diazoniumsalzen. 
27. A. Philippi, S. C. Lowder, F. Lamprecht and F. Gross: Effects of posture 
and exercise on the urinary clearance of renin in man. 
28. H. P. Nast und A. Distler: Untersuchungen über den Abbau von Angiotensin 
in menschlichen Erythrozyten. 
29. H. C. Siemensen: Untersuchungen an der Ratte zur Bedeutung der Leber 
für die Renin-Angiotensin-Kinetik. 
30. H. Wernze, A. Seki and R. Jesse: Hepatic renin extraction in hypertension 
and cirrhosis of the liver. 
31. R. Beckerhoff, J. A. Luetscher and W. Siegenthaler: Changes in renin-
angiotensin system induced by oral contraceptives. 
Aldosteron 
32. W. Vetter, E. Freedlender and E. Haber: Production and characterization of 
antibodies elicited by different aldosterone-protein conjugates. 
33. P. Vecsei, C. Akangbou, A. Joumaah and N. I. Salloum: Studies on anti-
bodies against corticoid hormones. 
VI 
34. W. R. Külpmann, L . Siekmann and H. Breuer: An improved gas chromato-
graphic method for the determination of aldosterone in urine. 
35. K. A. Deck, P. K. Champion, D. R. Rovner and / . W. Conn: Urinary free 
aldosterone in normal persons and in patients with primary aldosteronism. 
36. D. Lommer, A. Distler, T. Philipp and H. P. Wolff: Secretion, distribution 
and turnover of aldosterone in essential hypertension, primary aldosteronism, 
and hypertension associated with renal artery stenosis. 
Prolaktin 
37. M. UHermite, V. Stavric and C. Robyn: Human pituitary prolactin during 
pregnancy and post partum as measured in serum by a radioimmunoassay. 
38. P. Berle und K-D. Voigt: Plasma-Prolaktinkonzentrationen beim Mamma-
karzinom der Frau. 
39. D. Schams, V. Reinhardt and H. Karg: Effects of a synthetic ergot alkaloid 
on the peripheral blood level of prolactin in cows. 
40. R. Wenner und L . Varga: Prolactinhemmung mittels Ergocryptin. 
41. M. Little: The effect of prolactin and growth hormone preparations on the 
casein production in mouse mammary gland tissue explants. 
42. U. Hachmeister, R. Fahlbusch and K. v. Werder: Ultrastructural identity of 
pituitary adenoma cells in Forbes-Albright syndrome and of adenohypo-
physeal pregnancy cells. 
Wachstumshormon 
43. K.v. Werder, M. Gottsmann, A. Souvatzoglou, P. Botterman and K. Schwarz: 
Growth hormone and growth hormone-like immunoreactivity in man. 
44. P. H. Althoff, B. Schneider, K. M. Bartelt, K. Retiene und K. Schaffung: 
Der Glucagon-Stimulationstest, eine Methode zur Klassifizierung der Akro-
megalien — eine primäre, hypophysäre und eine sekundäre, hypothalamische 
Form? 
45. A. von zur Mühlen, J. Köbberling and A. Bachmann: The effect of repeated 
depot A C T H injections on arginine-induced H G H release in healthy subjects 
and patients with Addison's disease. 
46. W. Wiegelmann, H. G. Solbach, H. Bethge and H. Zimmermann: Growth 
hormone response to insulin-induced hypoglycaemia in anorexia nervosa. 
47. H. Nowakowski, R. Kautzky, N. Stahnke and B. Regler: Selective removal 
of pituitary adenomas in acromegaly. 
48. K. Hübner, K. H. Usadel and R. Schneider: Histoautoradiographic investi-
gation on the influence of hypophysectomy and STH-substitution on the 
regeneration of renal tubular epithelium following temporary ischemia. 
Adiuretin 
49. H. D. Freisenhausen and H. Frahm: Development of a radioimmunoassay 
for vasopressin. 
50. H. U.Tietze, A. Chattas and O. Oetliker: Behavior of free water clearance 
in children with vasopressin-sensitive diabetes insipidus and in children with 
renal diabetes insipidus under treatment with carbamyl-dibenzo-azepine 
(Tegretal). 
VI I 
51. F. Ublieb, K. Loeschke and / . Eigler: Antidiuretic effect of Carbamazepin 
in central diabetes insipidus. 
52. K. Irmscher, W. Wiegelmann, H. Bethge, J. Kampf jmeier, H. G. Solbach 
and H. Zimmermann: Therapeutic response of central diabetes insipidus on 
nasal application of a new synthetic analogue of vasopressin (l-deamino-8-
d-arginine-vasopressin (DDAVP). 
Gonadotropin-Releasing-Faktoren 
53. F. Enzmann, K. Geisen, R. Geiger, W. König and H. Wissmann: Isolation 
and characterization of two different porcine hypothalamic preparations 
with LH-RH-like activity and their comparison with synthetic LH-RH. 
54. / . Sandow, A. V. Schally, H. G. Schröder, W. Heptner and F. Enzmann: 
FSH-releasing activity of a synthetic decapeptide (LH/FSH-RH). 
55. S. Heller, H. D. Schlumberger, F. Kümmel and U. Maier: FSH-release by 
L-3-O-methyl-dopa and L-dopa. 
56. H. G. Schröder, K. Engelbart, J. Sandow and W. Heptner: The effect of 
synthetic LH/FSH-RH on the maturation of infantile female rats. 
57. A. von zur Mühlen and / . Köbberling: The effect of synthetic luteinizing 
hormone releasing factor (LH-RF) on the release of hormones of the 
adenohypophysis in normal men. 
58. K. Seeger, /. Sandow, H. G. Schröder and W. Heptner: Effect of synthetic 
LH/FSH-RH on LH-release and ovarian function in sheep. 
59. H. Kühl, W. Bichel, /. Enenkel, G. Hauptmann, P. Chandra and H.-D. 
Taubert: Contrasting effects of some steroidal hormones upon L-cystine-
aminopeptidase activitiy in the hypothalamus of immature male und female 
rats. 
60. A. Souvatzoglou, P. Bottermann, K. v. Werder and K. Schwarz: Effect of 
L-dopa on L H and G H levels in man. 
61. F. Lehmann, F. Peters, M. Breckwoldt and G. Bettendorf: Plasmaprogestins 
during infusion of prostaglandin F20C 
Calcitonin, Parathormon 
62. F. Gniffke, P. O. Schwüle and H. Bünte: Ionized calcium by selective 
electrode assay, normal range, loading tests. 
63. K. Paschen, C. Fuchs and R-D. Hesch: A simple and precise method for 
determination of ionized calcium in serum by an ionselective electrode. 
64. R. Hehrmann, J. Hagemann and R. Montz: Studies on endocrine regulations 
of intestinal calcium absorption. 
65. M. Hüfner, R.-D. Hesch, H. Schmidt, M. Hasenjäger, K. Winckler, W. 
Creutzfeldt and K. Paschen: The gastrointestinal effects of calcitonin. 
66. C. Lozano-Tonkin, H.-P. Kruse und H.-R. Montz: Auswertung klinischer 
und mikroradiographischer Befunde beim Hyperparathyreoidismus sowie 
ihre Korrelation zu Ergebnissen der 4 7Calcium-Kinetik. 
67. R. Montz, R. Hehrmann, F. Kuhlencordt and C. Schneider: 4 7Calcium ki-
netics in the evaluation of parathyroid function. 
VI I I 
68. E. Altenähr, R. Hehrmann and H-P. Kruse: Correlation of ultrastructural 
criteria of human parathyroid gland function with calcium and bone 
metabolism in hyperparathyroidism. 
69. G. Delling: Effect of calcitonin on increased bone resorption and osteoporotic 
changes induced by chronic acid feeding in the rat. 
70. H. van Lessen, G. Rodeck and H. Bechtelsheimer: Medullary carcinoma of 
the thyroid and hyperparathyroidism. 
71. F. Raue, H. Minne, S. Bellwinkel and R. Ziegler: Studies on the hypercalcemic 
syndrome in rats with Walker carcinosarcoma 256. 
HVL-NNR-System 
72. K. H. Voigt, H. L . Fehm and E. F. Pfeiffer: Extraction of ACTH from 
plasma for use in radioimmunoassay. 
73. H. L . Fehm, K. H. Voigt and £. F. Pfeiffer: Spontaneous and stimulated 
secretion of immunoreactive A C T H in man. 
74. / . Homoki and W. Teller: Studies on cortisol/corticosterone ratio in plasma 
as indirect measure of 17a-hydroxylase activity during childhood. 
75. W. Winkelmann, D. Hessen and R. Mies: 11-desoxycortisol and Cortisol pro-
duction in congenital adrenocortical syndrome. 
76. G. Zieger: Features of growth in the capsular area of the human adrenal 
cortex. 
77. K. J. Beck, G. Leyendecker and W. Nocke: Comparison of oestrogen con-
centration in serum and cervical mucus. 
78. Ch. Lauritzen and W. D. Lehmann: The feto-placental D H A load test: 
bases, results, interpretation. 
79. K. Wenzel, W. Obolensky and P. Vulliemoz: DHEAS-test and nutritional 
insufficieny of the placenta. 
80. W. Obolensky and K. Wenzel: Changes in estriol concentration in amniotic 
fluid after DHEAS-loading. 
81. W. Geiger and W. Post: The distribution of HCG, HCS, STH and TSH to 
various physiological fluids of mother and fetus in the last trimester of 
pregnancy. 
82. B. Hoffmann, D. Schams and H. Karg: Simultaneous determination of 
progesterone, total oestrogens, corticoids, prolactin and L H in peripheral 
blood of the cow around parturition. 
Katecholamine 
83. R. Gugler, ] . H, Hengstmann and H. J. Dengler: Clinical and biochemical 
findings in a malignant pheochromocytoma treated with alpha-methyl-p-
tyrosine. 
84. W. Gusek und M. Fock: Ultrastruktur hormondifferenter Phäochromo-
zytome. 
85. P. Ball, R. Knuppen, W. Wennrich and H. Breuer: Interactions between 
oestrogens and catecholamines: influence of oestrogens on the effect of 
catecholamines on blood pressure in rats. 
86. F. W. Schmahl, F. Buchholz, K. Huth and B. Urbaschek: Effects of 
norepinephrine and orciprenaline on endotoxin-induced alterations of blood 
lipids. 
I X 
Diabetes mellitus 
87. H. Schatz, Y. Abdel Rahman, M. Hinz, V. Maier, C. Nierle and E. F. 
Pfeiffer: The effect of growth hormone (GH) and adrenocorticotrophic 
hormone (ACTH) on secretion and biosynthesis of insulin in isolated islets 
of the rat. 
88. H. Laube, E. Grajeda, R. Fussgänger and E. F. Pfeiffer: Influence of 
adrenergic receptors on glucose-induced hyperinsulinemia of obese mice. 
89. W. Fechner and M. Uage: Development of acute diabetic ketoacidosis and 
its correlation to plasma corticosterone levels in the alloxanized rat. 
90. G. Klöppel, E. Altenähr and G. Freytag: Electronmicroscopic studies on 
cellular autoimmunity against insulin in the pancreas of rabbits. 
91. H. v. Lilienfeld-Toal, M. Hüfner, C. Fuchs and R. D. Hesch: Radio-
receptorassay for 3', 5'-AMP. 
92. P. O. Schwüle and B. Barth: Clearance and stop flow studies in glucagon 
mediated hyperelectrolyturia. 
Androgene, Hirsutismus 
93. E. Nieschlag, D. L . Loriaux and M. B. Lipsett: Radioimmunoassay for de-
ny droepiandrosterone and its sulfate: effect of ACTH, gonadotropins and 
dexamethasone on plasma levels. 
94. L . Penzes and G. W. Oertel: Estimation of oxosteroids by densitometry of 
suitable derivatives. 
95. W.-D. Hetzel, R. Kiehnscherf und W. Staib: Extraktion von Steroidkonju-
gaten durch flüssige Ionenaustauscher. 
96. D. Knorr und O. Butenandt: Hodenfunktionstest durch HCG-Stimulation 
und Plasmatestosteronbestimmung in der Vorpubertät. 
97. K.-P. Littmann und H. Gerdes: Testosterontagesrhythmik und HCG-Stimu-
lation bei akuten und chronischen Lebererkrankungen. 
98. W. M. Teller and H. Ferlemann: The influence of human chorionic gonado-
tropin (HCG) on urinary steroid patterns in prepubertal boys with distopic 
testes. 
99. K. H. Usadel, U. Schwedes, C. Schade, U. Leuschner und K. Schöffling: 
Nervenfasern in den Tubuluswänden der Hoden von Klinefelter-Patienten. 
Histochemische und elektronenoptische Befunde. 
100. P. Göbel, J. Cyran, V. Frick, A. E. Schindler und M. Schwarz: Anti-
androgentherapie des Hirsutismus. 
Gonadotropine 
101. D. Graesslin, H. Gh. Weise, W. Braendle and G. Bettendorf: Isolation and 
partial characterisation of human pituitary FSH and L H . 
102. L . Wildt, G. Leyendecker and W. Nocke: Studios on gel filtration of plasma 
L H and FSH. 
103. H. Gh. Weise, D. Graesslin, P.-J. Czygan and W. Braendle: Biological, 
immunological and physicochemical properties of several different HCG 
components. 
104. W. Braendle, P.-J. Czygan, D. Graesslin and H. Ch. Weise: Distribution and 
binding of 1 3 1 J-HCG in different organs of the female rat. 
X 
105. W. Geiger: The role of standard preparations and antisera in the differences 
of "absolute" LH-measurement. 
106. W. Heptner, E. Niemann, J. Sandow, N. Seher and F. Enzmann: Studies 
on the reactivity of fractions of 125 I-labelled ovine L H in radioimmuno-
assay and bioassay. 
107. H. Hildebrandt, M. Vekemans and C. Robyn: Radioimmunoassays for 
human chorionic gonadotropin (HCG) and human pituitary luteinizing 
hormone (HLH) using the double antibody method: comparative evaluation 
of equilibrium and non-equilibrium conditions. 
108. P-J. Czygan: Different feedback action of oestrogens in pre- and post-
menopausal women. 
109. D. Lindenmeyer and / . Tamm: Plasma levels of L H / H C G in male subjects 
following intravenous administration of HCG or oral treatment with 
Clomiphen. 
110. D. Schams, H. D. Butz and H. Karg: Sex differences on plasma luteinizing 
hormone level from birth to puberty in the bovine. 
X I 
Acta endocr. (Kbh.) 
Suppl. 159 (1972) 7 
18. Symp. Dtsch. Ges. Endokrin. 
Abstract No. 7 
I I . Medizinische Klinik der Universität München 
AUTOMATIZATION OF SERUM THYROXINE DETERMINATION 
A N D OF Ts-UPTAKE TEST* 
/. Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba 
Problem: 
Simple, rapid and inexpensive techniques were developed, since in this labora-
tory the requests for determinations of T4 an of T3-uptake exceed the number 
of 200 per week. This study was aimed at the development and the automatization 
of a competitive protein binding method for T4 on the basis of the automatic 
T3-uptake test in use. 
Methods: 
1. Ts-uptake-test: 0.2 ml serum and 0.4 ml T 3 - 1 2 5 J (0.1 ^Ci) in 0.5% albu-
min, 0.05 M sodium phosphate buffer p H 7.4 were equilibrated for 15 min at 
room temperature. 25 samples were simultaneously aspirated by a 25-channel 
peristaltic pump and delivered to 25 microcolumns containing 1 g sephadex G-25 
fine, placed in a thermoblock (29° C) for separation of protein-bound and so-
called free = dextran gel adsorbed T 3 - 1 2 5 J . The latter was totally eluted by 
pooled serum. With this procedure the same columns could be used for weeks. 
The whole separation was performed automatically within 25 min with a time-
controlled apparatus. 
2. Serum-Ti: 0.1 ml serum and 0.3 ml T 4 - 1 2 5 J (0.1 ^Ci) in 0.5% albumin, 
0.05 M sodium phosphate buffer ph 7.4 are incubated for 15 min at room 
temperature. The incubation mixtures are delivered as above to 1 g sephadex G-25 
s. f. columns, which are alkalized with 0.02 N NaOH, resulting in complete 
retention of cold endogenous and radioactive T4. The columns are then washed 
with 0.05 M sodium barbital buffer p H 8.6. Addition of 1.6 ml pregnancy serum 
(11:00 diluted in barbital buffer) caused competitive protein binding for T4 on 
the column and simultaneously eluted so bound T4. The relative radioactivity in 
this eluate indicated the amount of endogenous T4. The remaining hormones were 
finally totally eluted from the columns with excess serum, so that re-use of the 
columns was possible. For this CPB-method for serum T4, the same automatic 
apparatus as for the T3-uptake test is used. 
Results: 
The normal range of Ts-uptake (x ± 2 s) is 32—42%; quality control is 
satisfactory with a variation coefficient of 3.5% for a control serum checked for 
more than 2 years. Clinical data and quality control of the automatic CPB-
method for T4 wi l l be presented. 
* Supported by the Deutsche Forschungsgemeinschaft (SFB 51). 
7 


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Automatization of serum thyroxine determination and of T3-uptake test

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