Erhardt, F.

Fahlbusch, R.

Geiger, W.

Heinze, H. G.

Pickardt, C. R.

Scriba, Peter Christian

English

Universität München: Elektronischen Publikationen

Acta 
endocrinologica 
Supplementum 159 
A D V A N C E A B S T R A C T S 
O F 
SHORT PAPERS 
18. Symposion Deutsche Gesellschaft für Endokrinologie 
Unter Mitwirkung der Deutschen Gesellschaft für Klinische 
Chemie 
Hannover 
March 1-4, 1972 
P E R I O D I C A - C O P E N H A G E N 1972 
C O N T E N T S 
7 RH, 7SH, Schilddrüse 
1. C. R. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime and 
P. C. Scriba: TRF-stimulation-test in hypothalamic and pi tui tary diseases, 
and i n autonomous adenoma of the thyroid . 
2. G . Rothenbuchner, J. Birk, U. Loos, S. Raptis and E. F. Pfeiffer: The 
dynamics of TSH-secretion after oral administration of synthetic thyro-
tropin-releasing factor (TRF) in metabolically normal subjects. 
3. G. Rothenbuchner, U. Loosy G. Knapp, I. Birk, W. R. Kießling and E. F. 
Pfeiffer: The alterations of total-thyroxine and total-triiodothyronine 
after oral application of synthetic thyrotropin-releasing factor (TRF) in 
metabolically normal subjects. 
4. K. Retiene, H. Holz, A, Müller, R. Guthoff, H.-J. Ewers, K. M. Bartelt, 
P. Althoff and V. Grabs: Further experimental und clinical findings during 
long-term administration of synthetic T R H . 
5. F. Adlkofer, H. Schleusener and A. von zur Mühlen: T H S activity in 
extracts from hyperthyroid plasma before and after administration of 
thyrotropin-releasing factor ( T R H ) . 
6. R.-D. Hesch, M. Hüfner and A. v. z. Mühlen: Radioimmunoassay of 
tr i iodothyronine in thyro id diseases. 
7. / . Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba: Auto-
matization of serum thyroxine determination and of T3-uptake test. 
8. K. Horn, H. Borowzak, J. Habermann, M. Rettig, 7. Ruhl and P. C. Scriba: 
Tota l serum Ta and T 4 in nontoxic goiter and autonomous adenoma. 
9. K. Hackenberg, R. Schneider and D. Reinwein: Thyroidal suppressibility 
and thyroglobulin antibodies during treatment of thyrotoxicosis w i t h anti-
t hy ro id drugs. 
10. F. W. Lohmann, H. Schleusener and F. Adlkofer: The sympathoadrenal 
system i n hyperthyroidism. 
11. / . Nolte, D. Brdiczka, P. C. Scriba und H. W. Staudte: Schilddrüsen-
hormonwirkung im Riboflavinmangel. 
12. K. Schimmelpfennig, A. Kaul and E. Köhler: Problems connected wi th in -
vestigations of athyroid experimental animals. 
SteroidstoffWechsel und -Wirkungen 
13. W. Mestwerdt, H. Brandau und H. H. Kley: Struktur und Funktion steroid-
aktiver Zellen im Postmenopauseovar. 
14. M. Breckwoldt, W. Wortmann, ] . C. Touchstone and B. P. Lisboa: In -v ivo 
perfusion of the kidney w i t h 3H-estradiol-17/? in the rhesus monkey. 
V 
15. P. Benes and G. W. Oertel: Penetration of intact mitochondria by 
dehydroepiandrosterone, its conjugates or derivatives. 
16. H. Brandau und G. Mutzke: Akt iv i tä t smuster von H y d r o x y steroid-Dehy-
drogenasen i m Corpus luteum des Rindes. 
17. Jeanne Sloe Eng Dericks-Tan: Metabolization of ethinyl-nor-testosterone 
acetate by female rat liver microsomes. 
18. D. Engelhardt, P. Unterburger and H. J. Karl: Studies of Ci9-steroid-
enzyme activities in normal and pathological human liver tissue. 
19. R. Ghraf, E. R. Lax, H. Ockenfels and H. Schrie fers: Sex- and age-
dependent hydroxylations of testosterone i n the liver of normally developed 
rats and in animals w i t h disturbed sexual development. 
20. B. P. Lisboa and J.-C. Plasse: zl5-3a-hydroxysteroid-dehydrogenase act ivi ty 
i n the human fetal l iver. 
21 . H. Becker, E. Grabosch and K. Voigt: Metabolism and mode of action 
of androgens i n target tissues of male rats 1. Metabolism of 4a-androstan-
3ß, 17/?-diol in target organs and in peripheral tissues. 
22. H. Schmidt, G. v. Rotteck and K. D. Voigt: Metabolism and mode of action 
of androgens in target tissues of male rats 2. Mode of action of 5a-
androstan-3/?, 17/?-diol i n target organs and peripheral tissues. 
23. H-G. Hoff, R. Ghraf and H. Schriefers: Androgen-dependency of the 
sex-specific differentiation of the act ivi ty of enzymes involved i n steroid 
metabolism. 
24. G. Trams, P. Br Ummer and H. Maass: Studies on the interrelationship be-
tween oestrogens and gestagens in the target tissue. 
25. Ch. Seyfried and H-G. Kraft: Nucleic acid metabolism in the rat uterus: 
effect of E M D 16.795, a competitive anti-oestrogen. 
Renin, Angiotensin 
26. W. Vogt, Kh. R. Koczorek, H.-H. Vogt und M. Knedel: Polypeptid-
hormonnachweis durch Kupplung mit Diazoniumsalzen. 
27. A. Philippi, S. C. Lowder, F. Lamprecht and F. Gross: Effects of posture 
and exercise on the urinary clearance of renin i n man. 
28. H. P. Nast und A. Distler: Untersuchungen über den Abbau von Angiotensin 
in menschlichen Erythrozyten. 
29. H. C. Siemensen: Untersuchungen an der Ratte zur Bedeutung der Leber 
für die Renin-Angiotensin-Kinetik. 
30. H. Wernze, A. Seki and R. Jesse: Hepatic renin extraction in hypertension 
and cirrhosis of the liver. 
31. R. Beckerhoff, J. A. Luetscher and W. Siegenthaler: Changes in renin-
angiotensin system induced by oral contraceptives. 
Aldosteron 
32. W. Vetter, E. Freedlender and E. Haber: Production and characterization of 
antibodies elicited by different aldosterone-protein conjugates. 
33. P. Vecsei, C. Akangbou, A. Joumaah and N. I. Salloum: Studies on anti-
bodies against corticoid hormones. 
VI 
34. W. R. Külpmann, L . Siekmann and H. Breuer: A n improved gas chromato-
graphic method for the determination of aldosterone in urine. 
35. K. A. Deck, P. K. Champion, D. R. Rovner and / . W. Conn: Ur inary free 
aldosterone in normal persons and i n patients w i t h primary aldosteronism. 
36. D. Lommer, A. Distler, T. Philipp and H. P. Wolff: Secretion, distribution 
and turnover of aldosterone in essential hypertension, primary aldosteronism, 
and hypertension associated w i t h renal artery stenosis. 
Prolaktin 
37. M. UHermite, V. Stavric and C. Robyn: Human pi tui tary prolactin during 
pregnancy and post partum as measured in serum by a radioimmunoassay. 
38. P. Berle und K.-D. Voigt: Plasma-Prolaktinkonzentrationen beim Mamma-
karzinom der Frau. 
39. D. Schams, V. Reinhardt and H. Karg: Effects of a synthetic ergot alkaloid 
on the peripheral blood level of prolactin in cows. 
40. R. Wenner und L . Varga: Prolactinhemmung mittels Ergocryptin. 
4 1 . M. Little: The effect of prolactin and growth hormone preparations on the 
casein production in mouse mammary gland tissue explants. 
42. U. Hachmeister, R. Fahlbusch and K. v. Werder: Ultrastructural identity of 
pi tui tary adenoma cells in Forbes-Albright syndrome and of adenohypo-
physeal pregnancy cells. 
Wachstumshormon 
43. K. v. Werder, M. Gottsmann, A. Souvatzoglou, P. Botterman and K. Schwarz: 
Growth hormone and growth hormone-like immunoreactivity in man. 
44. P. H. Althoff, B. Schneider, K. M. Bartelt, K. Retiene und K. Schaffung: 
Der Glucagon-Stimulationstest, eine Methode zur Klassifizierung der A k r o -
megalien — eine pr imäre , hypophysä re und eine sekundäre, hypothalamische 
Form? 
45. A. von zur Mühlen, J. Köbberling and A, Bachmann: The effect of repeated 
depot A C T H injections on arginine-induced H G H release in healthy subjects 
and patients w i t h Addison's disease. 
46. W. Wiegelmann, H. G. Solbach, H. Bethge and H. Zimmermann: Growth 
hormone response to insulin-induced hypoglycaemia in anorexia nervosa. 
47. H. Nowakowski, R. Kautzky, N. Stahnke and B. Regler: Selective removal 
of pi tui tary adenomas in acromegaly. 
48. K. Hübner, K. H. Usadel and R. Schneider: Histoautoradiographic investi-
gation on the influence of hypophysectomy and STH-substitution on the 
regeneration of renal tubular epithelium fol lowing temporary ischemia. 
Adiuretin 
49. H. D. Freisenhausen and H. Frahm: Development of a radioimmunoassay 
for vasopressin. 
50. H. U.Tietze, A. Chattas and O. Oetliker: Behavior of free water clearance 
in children w i t h vasopressin-sensitive diabetes insipidus and in children w i t h 
renal diabetes insipidus under treatment w i t h carbamyl-dibenzo-azepine 
(Tegretal). 
VI I 
51 . F. Ublieb, K. Loeschke and / . Eigler: Antidiuretic effect of Carbamazepin 
i n central diabetes insipidus. 
52. K. Irmscher, W. Wiegelmann, H. Bethge, J. Kampffmeier, H. G. Solbach 
and H. Zimmermann: Therapeutic response of central diabetes insipidus on 
nasal application of a new synthetic analogue of vasopressin (l-deamino-8-
d-arginine-vasopressin ( D D A V P ) . 
Gonadotropin-Releasing-Faktoren 
53. F. Enzmann, K. Geisen, R. Geiger, W. König and H. Wissmann: Isolation 
and characterization of two different porcine hypothalamic preparations 
w i t h L H - R H - l i k e activity and their comparison w i t h synthetic L H - R H . 
54. / . Sandow, A V. Scbally, H. G. Schröder, W. Heptner and F. Enzmann: 
FSH-releasing act ivi ty of a synthetic decapeptide ( L H / F S H - R H ) . 
55. S. Heller, H. D. Schlumberger, F. Kümmel and U. Maier: FSH-release by 
L-3-O-methyl-dopa and L-dopa. 
56. H. G. Schröder, K. Engelbart, ] . Sandow and W. Heptner: The effect of 
synthetic L H / F S H - R H on the maturation of infantile female rats. 
57. A. von zur Mühlen and / . Köbberling: The effect of synthetic luteinizing 
hormone releasing factor (LH-RF) on the release of hormones of the 
adenohypophysis in normal men. 
58. K. Seeger, ] . Sandow, H. G. Schröder and W. Heptner: Effect of synthetic 
L H / F S H - R H on LH-release and ovarian function in sheep. 
59. H. Kühl, W. Bichel, /. Enenkel, G. Hauptmann, P. Chandra and H-D. 
Taubert: Contrasting effects of some steroidal hormones upon L-cystine-
aminopeptidase act ivi t iy in the hypothalamus of immature male und female 
rats. 
60. A, Souvatzoglou, P. Bottermann, K. v. Werder and K. Schwarz: Effect of 
L-dopa on L H and G H levels in man. 
61 . F. Lehmann, F. Peters, M. Breckwoldt and G. Bettendorf: Plasmaprogestins 
during infusion of prostaglandin F 2 « 
Calcitonin, Parathormon 
62. F. Gniffke, P. O. Schwüle and H. Bünte: Ionized calcium by selective 
electrode assay, normal range, loading tests. 
63. K. Paschen, C. Fuchs and R-D, Hescb: A simple and precise method for 
determination of ionized calcium in serum by an ionselective electrode. 
64. R. Hehrmann, J. Hagemann and R. Montz: Studies on endocrine regulations 
of intestinal calcium absorption. 
65. M. Hüfner, R.-D. Hescb, H. Schmidt, M. Hasenjäger> K. Winckler, W. 
Creutzfeldt and K. Paschen: The gastrointestinal effects of calcitonin. 
66. C. Lozano-Tonkin, H.-P. Kruse und H.-R. Montz: Auswertung klinischer 
und mikroradiographischer Befunde beim Hyperparathyreoidismus sowie 
ihre Korrelat ion zu Ergebnissen der 4 7 Calc ium-Kinet ik . 
67. R. Montz, R. Hehrmann, F. Kuhlencordt and C. Schneider: 4 7 Calc ium k i -
netics i n the evaluation of parathyroid function. 
VI I I 
68. E, Altenahr; R. Hehrmann and H-P. Kruse: Correlation of ultrastructural 
criteria of human parathyroid gland function w i t h calcium and bone 
metabolism in hyperparathyroidism. 
69. G. Delling: Effect of calcitonin on increased bone resorption and osteoporotic 
changes induced by chronic acid feeding in the rat. 
70. H. van Lessen, G. Rodeck and H. Bechtelsheimer: Medullary carcinoma of 
the thyroid and hyperparathyroidism. 
71 . F. Raue, H. Minne, S. Bellwinkel and R. Ziegler: Studies on the hypercalcemic 
syndrome in rats w i t h Walker carcinosarcoma 256. 
HVL-NNR-System 
72. K. H. Voigt, H. L . Fehm and E. F. Pfeiffer: Extraction of A C T H from 
plasma for use in radioimmunoassay. 
73. H. L . Fehm, K. H. Voigt and £ . F. Pfeiffer: Spontaneous and stimulated 
secretion of immunoreactive A C T H in man. 
74. / . Homoki and W. Teller: Studies on cortisol/corticosterone ratio in plasma 
as indirect measure of 17a-hydroxylase activi ty during childhood. 
75. W. Winkelmann, D. Hessen and R. Mies: 11-desoxycortisol and Cortisol pro-
duction in congenital adrenocortical syndrome. 
76. G. Zieger: Features of growth in the capsular area of the human adrenal 
cortex. 
77. K. J. Beck, G. Leyendecker and W. Nocke: Comparison of oestrogen con-
centration in serum and cervical mucus. 
78. Ch. Lauritzen and W. D. Lehmann: The feto-placental D H A load test: 
bases, results, interpretation. 
79. K. Wenzel, W. Obolensky and P. Vulliemoz: DHEAS-test and nutr i t ional 
insufficieny of the placenta. 
80. W . Obolensky and K. Wenzel: Changes in estriol concentration in amniotic 
f l u id after DHEAS-loading. 
81. W. Geiger and W. Post: The distribution of H C G , HCS, S T H and T S H to 
various physiological fluids of mother and fetus in the last trimester of 
pregnancy. 
82. B. Hoffmann, D. Schams and H. Karg: Simultaneous determination of 
progesterone, total oestrogens, corticoids, prolactin and L H in peripheral 
blood of the cow around parturi t ion. 
Katecholamine 
83. R. Gugler, J. H. Hengstmann and H. J. Dengler: Clinical and biochemical 
findings in a malignant pheochromocytoma treated w i t h alpha-methyl-p-
tyrosine. 
84. W. Gusek und M. Fock: Ultrastruktur hormondifferenter Phäoch romo-
zytome. 
85. P. Ball, R. Knuppen, W. Wennrich and H. Breuer: Interactions between 
oestrogens and catecholamines: influence of oestrogens on the effect of 
catecholamines on blood pressure in rats. 
86. F. W. Schmahl, F. Buchholz, K. Huth and B. Urbaschek: Effects of 
norepinephrine and orciprenaline on endotoxin-induced alterations of blood 
lipids. 
I X 
Diabetes mellitus 
87. H. Schatz, Y. Abdel Rahman, M. Hinz, V . Maier, C. Nierle and E. F. 
Pfeiffer: The effect of growth hormone ( G H ) and adrenocorticotrophic 
hormone ( A C T H ) on secretion and biosynthesis of insulin in isolated islets 
of the rat. 
88. H. Laube, E. Grajeda, R. Fussgänger and E. F. Pfeiffer: Influence o f 
adrenergic receptors on glucose-induced hyperinsulinemia of obese mice. 
89. W. Fechner and M. Vage: Development of acute diabetic ketoacidosis and 
its correlation to plasma corticosterone levels in the alloxanized rat. 
90. G. Klöppel, E. Altenähr and G. Freytag: Electronmicroscopic studies on 
cellular autoimmunity against insulin in the pancreas of rabbits. 
91 . H. v. Lilienfeld-Toal, M. Hüfner, C. Fuchs and R. D. Hescb: Radio-
receptorassay for 3', 5 ' -AMP. 
92. P. O. Schwüle and B. Barth: Clearance and stop f low studies in glucagon 
mediated hyperelectrolyturia. 
Androgene, Hirsutismus 
93. E. Nieschlag, D. L . Loriaux and M. B. Lipsett: Radioimmunoassay for de-
ny droepiandrosterone and its sulfate: effect of A C T H , gonadotropins and 
dexamethasone on plasma levels. 
94. L . Penzes and G. W. Oertel: Estimation of oxosteroids by densitometry of 
suitable derivatives. 
95. W.-D. Hetzel, R. Kiehnscherf und W. Staib: Extrakt ion von Steroidkonju-
gaten durch flüssige Ionenaustauscher. 
96. D. Knorr und O. Butenandt: Hodenfunktionstest durch HCG-Stimulat ion 
und Plasmatestosteronbestimmung in der Vorpuber t ä t . 
97. K.-P. Littmann und H. Gerdes: Testosterontagesrhythmik und HCG-St imu-
lat ion bei akuten und chronischen Lebererkrankungen. 
98. W. M. Teller and H. Ferlemann: The influence of human chorionic gonado-
tropin ( H C G ) on urinary steroid patterns in prepubertal boys w i t h distopic 
testes. 
99. K. H. Usadel, U. Schwedes, C. Schade, U. Leuschner und K. Schöffling: 
Nervenfasern in den Tubuluswänden der Hoden von Klinefelter-Patienten. 
Histochemische und elektronenoptische Befunde. 
100. P. Göbel, J. Cyran, V. Frick, A. E. Schindler und M. Schwarz: A n t i -
androgentherapie des Hirsutismus. 
Gonadotropine 
101. D. Graesslin, H. Gh. Weise, W. Braendle and G. Bettendorf: Isolation and 
part ial characterisation of human pi tu i tary FSH and L H . 
102. L . Wildt, G. Leyendecker and W. Nocke: Studios on gel f i l t ra t ion of plasma 
L H and FSH. 
103. H. Gh. Weise, D. Graesslin, P.-J. Czygan and W. Braendle: Biological, 
immunological and physicochemical properties of several different H C G 
components. 
104. W. Braendle, P.-J. Czygan, D. Graesslin and H. Ch. Weise: Distribution and 
binding of 1 3 1 J - H C G i n different organs of the female rat. 
X 
105. W. Geiger: The role of standard preparations and antisera in the differences 
of "absolute" LH-measurement. 
106. W. Heptner, E. Niemann, J. Sandow, N. Seher and F. Enzmann: Studies 
on the reactivity of fractions of 125 I-labelled ovine L H in radioimmuno-
assay and bioassay. 
107. H. Hildebrandt, M. Vekemans and C. Robyn: Radioimmunoassays for 
human chorionic gonadotropin ( H C G ) and human pi tui tary luteinizing 
hormone ( H L H ) using the double antibody method: comparative evaluation 
of equilibrium and non-equilibrium conditions. 
108. P-J. Czygan: Different feedback action of oestrogens in pre- and post-
menopausal women. 
109. D. Lindenmeyer and / . Tamm: Plasma levels of L H / H C G in male subjects 
fol lowing intravenous administration of H C G or oral treatment w i t h 
Clomiphen. 
110. D. Schams, H. D. Butz and H. Karg: Sex differences on plasma luteinizing 
hormone level from bi r th to puberty in the bovine. 
XI 
18. Symp. Dtsch. Ges. Endokrin. 
Abstract N o . 1 
Acta endocr. (Kbh.) 
Suppl. 159 (1972) 1 
I I . Medizinische K l i n i k der Univers i tä t München 
T R F - S T I M U L A T I O N - T E S T I N H Y P O T H A L A M I C A N D P I T U I T A R Y 
DISEASES, A N D I N A U T O N O M O U S A D E N O M A OF T H E T H Y R O I D 
C. R. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime 
and P. C. Scriba* 
Questions: 
1. What is the val id i ty of the TRF-stimulation test for the differentiation of 
hypothalamic and pi tui tary secondary hypothyroidism? 
2. What is the TSH-response to TRF-stimulation in patients w i t h „ autonomous 
adenoma" of the thyroid w i t h and without hyperthyroidism? 
Methods: 
500 ix% T R F was rapidly injected i . v . into hospitalized patients. Blood samples 
were wi thdrawn for T S H determinations before (—15 and —5 min) and after 
TRF-injection (5, 10, 20, 30, 60, 120 min and 24 h). Serum T S H levels were de-
termined radioimmunologically by a double antibody technique (1), using H T S H 
and a n t i - H T S H serum from N I H , Bethesda, and HTSH-Research Standard A 
from N I M R , London. 
Results: 
The TSH-response 30 min after injection of T R F was compared w i t h the 
integral of the TSH-response from zero up to 120 min. The use of the 120 min 
integral d id not improve differentiation between the groups as compared w i t h 
the 30 min value. 
The TSH-response after TRF-stimulation was absent or diminished in patients 
w i t h intrasellar tumors ( N = 12) after hypophysectomy, as compared to controls. 
I n 3 out of 9 patients w i t h intrasellar tumors studied preoperatively, secondary 
hypothyroidism existed. The TSH-response to T R F nevertheless was normal i n 
these 3 patients. This result decreases the value of the TRF-test for the differentia-
t ion of pi tui tary and hypothalamic secondary hypothyroidism. I n patients w i t h 
suprasellar tumors or hypothalamic diseases the TSH-response was above normal 
( N = 4), normal ( N = 2) or diminished ( N = 2). I n the latter 2 patients the an-
terior p i tu i tary was involved in the underlying disease. 
Further data obtained in patients w i t h acromegaly ( N = 13) and i n subjects w i t h 
autonomous adenoma of the thyro id w i l l be presented. 
1. Pickardt, C. R. et ai: K l i n . Wschr., i n pr in t . 
* Supported by the Deutsche Forschungsgemeinschaft (SFB 51). 
Supplemcntum 159 1 
1 


TRF-stimulation-test in hypothalamic and pituitary diseases, and in autonomous adenoma of the thyroid

Open Access LMU

Acta endocrinologica Supplementum 159 A D V A N C E A B S T R A C T S O F SHORT PAPERS 18. Symposion Deutsche Gesellschaft für Endokrinologie Unter Mitwirkung der Deutschen Gesellschaft für Klinische Chemie Hannover March 1-4, 1972 P E R I O D I C A - C O P E N H A G E N 1972 C O N T E N T S 7 RH, 7SH, Schilddrüse 1. C. R. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime and P. C. Scriba: TRF-stimulation-test in hypothalamic and pi tui tary diseases, and i n autonomous adenoma of the thyroid . 2. G . Rothenbuchner, J. Birk, U. Loos, S. Raptis and E. F. Pfeiffer: The dynamics of TSH-secretion after oral administration of synthetic thyro-tropin-releasing factor (TRF) in metabolically normal subjects. 3. G. Rothenbuchner, U. Loosy G. Knapp, I. Birk, W. R. Kießling and E. F. Pfeiffer: The alterations of total-thyroxine and total-triiodothyronine after oral application of synthetic thyrotropin-releasing factor (TRF) in metabolically normal subjects. 4. K. Retiene, H. Holz, A, Müller, R. Guthoff, H.-J. Ewers, K. M. Bartelt, P. Althoff and V. Grabs: Further experimental und clinical findings during long-term administration of synthetic T R H . 5. F. Adlkofer, H. Schleusener and A. von zur Mühlen: T H S activity in extracts from hyperthyroid plasma before and after administration of thyrotropin-releasing factor ( T R H ) . 6. R.-D. Hesch, M. Hüfner and A. v. z. Mühlen: Radioimmunoassay of tr i iodothyronine in thyro id diseases. 7. / . Habermann, J. Henner, K. Horn, I. zur Horst and P. C. Scriba: Auto-matization of serum thyroxine determination and of T3-uptake test. 8. K. Horn, H. Borowzak, J. Habermann, M. Rettig, 7. Ruhl and P. C. Scriba: Tota l serum Ta and T 4 in nontoxic goiter and autonomous adenoma. 9. K. Hackenberg, R. Schneider and D. Reinwein: Thyroidal suppressibility and thyroglobulin antibodies during treatment of thyrotoxicosis w i t h anti-t hy ro id drugs. 10. F. W. Lohmann, H. Schleusener and F. Adlkofer: The sympathoadrenal system i n hyperthyroidism. 11. / . Nolte, D. Brdiczka, P. C. Scriba und H. W. Staudte: Schilddrüsen-hormonwirkung im Riboflavinmangel. 12. K. Schimmelpfennig, A. Kaul and E. Köhler: Problems connected wi th in -vestigations of athyroid experimental animals. SteroidstoffWechsel und -Wirkungen 13. W. Mestwerdt, H. Brandau und H. H. Kley: Struktur und Funktion steroid-aktiver Zellen im Postmenopauseovar. 14. M. Breckwoldt, W. Wortmann, ] . C. Touchstone and B. P. Lisboa: In -v ivo perfusion of the kidney w i t h 3H-estradiol-17/? in the rhesus monkey. V 15. P. Benes and G. W. Oertel: Penetration of intact mitochondria by dehydroepiandrosterone, its conjugates or derivatives. 16. H. Brandau und G. Mutzke: Akt iv i tä t smuster von H y d r o x y steroid-Dehy-drogenasen i m Corpus luteum des Rindes. 17. Jeanne Sloe Eng Dericks-Tan: Metabolization of ethinyl-nor-testosterone acetate by female rat liver microsomes. 18. D. Engelhardt, P. Unterburger and H. J. 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Freedlender and E. Haber: Production and characterization of antibodies elicited by different aldosterone-protein conjugates. 33. P. Vecsei, C. Akangbou, A. Joumaah and N. I. Salloum: Studies on anti-bodies against corticoid hormones. VI 34. W. R. Külpmann, L . Siekmann and H. Breuer: A n improved gas chromato-graphic method for the determination of aldosterone in urine. 35. K. A. Deck, P. K. Champion, D. R. Rovner and / . W. Conn: Ur inary free aldosterone in normal persons and i n patients w i t h primary aldosteronism. 36. D. Lommer, A. Distler, T. Philipp and H. P. Wolff: Secretion, distribution and turnover of aldosterone in essential hypertension, primary aldosteronism, and hypertension associated w i t h renal artery stenosis. Prolaktin 37. M. UHermite, V. Stavric and C. Robyn: Human pi tui tary prolactin during pregnancy and post partum as measured in serum by a radioimmunoassay. 38. P. Berle und K.-D. Voigt: Plasma-Prolaktinkonzentrationen beim Mamma-karzinom der Frau. 39. D. Schams, V. Reinhardt and H. Karg: Effects of a synthetic ergot alkaloid on the peripheral blood level of prolactin in cows. 40. R. Wenner und L . Varga: Prolactinhemmung mittels Ergocryptin. 4 1 . M. Little: The effect of prolactin and growth hormone preparations on the casein production in mouse mammary gland tissue explants. 42. U. Hachmeister, R. Fahlbusch and K. v. Werder: Ultrastructural identity of pi tui tary adenoma cells in Forbes-Albright syndrome and of adenohypo-physeal pregnancy cells. Wachstumshormon 43. K. v. Werder, M. Gottsmann, A. Souvatzoglou, P. Botterman and K. Schwarz: Growth hormone and growth hormone-like immunoreactivity in man. 44. P. H. Althoff, B. Schneider, K. M. Bartelt, K. Retiene und K. Schaffung: Der Glucagon-Stimulationstest, eine Methode zur Klassifizierung der A k r o -megalien — eine pr imäre , hypophysä re und eine sekundäre, hypothalamische Form? 45. A. von zur Mühlen, J. Köbberling and A, Bachmann: The effect of repeated depot A C T H injections on arginine-induced H G H release in healthy subjects and patients w i t h Addison's disease. 46. W. Wiegelmann, H. G. Solbach, H. Bethge and H. Zimmermann: Growth hormone response to insulin-induced hypoglycaemia in anorexia nervosa. 47. H. Nowakowski, R. Kautzky, N. Stahnke and B. Regler: Selective removal of pi tui tary adenomas in acromegaly. 48. K. Hübner, K. H. Usadel and R. Schneider: Histoautoradiographic investi-gation on the influence of hypophysectomy and STH-substitution on the regeneration of renal tubular epithelium fol lowing temporary ischemia. Adiuretin 49. H. D. Freisenhausen and H. Frahm: Development of a radioimmunoassay for vasopressin. 50. H. U.Tietze, A. Chattas and O. Oetliker: Behavior of free water clearance in children w i t h vasopressin-sensitive diabetes insipidus and in children w i t h renal diabetes insipidus under treatment w i t h carbamyl-dibenzo-azepine (Tegretal). VI I 51 . F. Ublieb, K. Loeschke and / . Eigler: Antidiuretic effect of Carbamazepin i n central diabetes insipidus. 52. K. Irmscher, W. Wiegelmann, H. Bethge, J. Kampffmeier, H. G. Solbach and H. Zimmermann: Therapeutic response of central diabetes insipidus on nasal application of a new synthetic analogue of vasopressin (l-deamino-8-d-arginine-vasopressin ( D D A V P ) . Gonadotropin-Releasing-Faktoren 53. F. Enzmann, K. Geisen, R. Geiger, W. König and H. Wissmann: Isolation and characterization of two different porcine hypothalamic preparations w i t h L H - R H - l i k e activity and their comparison w i t h synthetic L H - R H . 54. / . Sandow, A V. Scbally, H. G. Schröder, W. Heptner and F. Enzmann: FSH-releasing act ivi ty of a synthetic decapeptide ( L H / F S H - R H ) . 55. S. Heller, H. D. Schlumberger, F. Kümmel and U. Maier: FSH-release by L-3-O-methyl-dopa and L-dopa. 56. H. G. Schröder, K. Engelbart, ] . Sandow and W. Heptner: The effect of synthetic L H / F S H - R H on the maturation of infantile female rats. 57. A. von zur Mühlen and / . Köbberling: The effect of synthetic luteinizing hormone releasing factor (LH-RF) on the release of hormones of the adenohypophysis in normal men. 58. K. Seeger, ] . Sandow, H. G. Schröder and W. Heptner: Effect of synthetic L H / F S H - R H on LH-release and ovarian function in sheep. 59. H. Kühl, W. Bichel, /. Enenkel, G. Hauptmann, P. Chandra and H-D. Taubert: Contrasting effects of some steroidal hormones upon L-cystine-aminopeptidase act ivi t iy in the hypothalamus of immature male und female rats. 60. A, Souvatzoglou, P. Bottermann, K. v. Werder and K. Schwarz: Effect of L-dopa on L H and G H levels in man. 61 . F. Lehmann, F. Peters, M. Breckwoldt and G. Bettendorf: Plasmaprogestins during infusion of prostaglandin F 2 « Calcitonin, Parathormon 62. F. Gniffke, P. O. Schwüle and H. 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Tamm: Plasma levels of L H / H C G in male subjects fol lowing intravenous administration of H C G or oral treatment w i t h Clomiphen. 110. D. Schams, H. D. Butz and H. Karg: Sex differences on plasma luteinizing hormone level from bi r th to puberty in the bovine. XI 18. Symp. Dtsch. Ges. Endokrin. Abstract N o . 1 Acta endocr. (Kbh.) Suppl. 159 (1972) 1 I I . Medizinische K l i n i k der Univers i tä t München T R F - S T I M U L A T I O N - T E S T I N H Y P O T H A L A M I C A N D P I T U I T A R Y DISEASES, A N D I N A U T O N O M O U S A D E N O M A OF T H E T H Y R O I D C. R. Pickardt, F. Erhardt, W. Geiger, R. Fahlbusch, H. G. Heime and P. C. Scriba* Questions: 1. What is the val id i ty of the TRF-stimulation test for the differentiation of hypothalamic and pi tui tary secondary hypothyroidism? 2. What is the TSH-response to TRF-stimulation in patients w i t h „ autonomous adenoma" of the thyroid w i t h and without hyperthyroidism? Methods: 500 ix% T R F was rapidly injected i . v . into hospitalized patients. Blood samples were wi thdrawn for T S H determinations before (—15 and —5 min) and after TRF-injection (5, 10, 20, 30, 60, 120 min and 24 h). Serum T S H levels were de-termined radioimmunologically by a double antibody technique (1), using H T S H and a n t i - H T S H serum from N I H , Bethesda, and HTSH-Research Standard A from N I M R , London. Results: The TSH-response 30 min after injection of T R F was compared w i t h the integral of the TSH-response from zero up to 120 min. The use of the 120 min integral d id not improve differentiation between the groups as compared w i t h the 30 min value. The TSH-response after TRF-stimulation was absent or diminished in patients w i t h intrasellar tumors ( N = 12) after hypophysectomy, as compared to controls. I n 3 out of 9 patients w i t h intrasellar tumors studied preoperatively, secondary hypothyroidism existed. The TSH-response to T R F nevertheless was normal i n these 3 patients. This result decreases the value of the TRF-test for the differentia-t ion of pi tui tary and hypothalamic secondary hypothyroidism. I n patients w i t h suprasellar tumors or hypothalamic diseases the TSH-response was above normal ( N = 4), normal ( N = 2) or diminished ( N = 2). I n the latter 2 patients the an-terior p i tu i tary was involved in the underlying disease. Further data obtained in patients w i t h acromegaly ( N = 13) and i n subjects w i t h autonomous adenoma of the thyro id w i l l be presented. 1. Pickardt, C. R. et ai: K l i n . Wschr., i n pr in t . * Supported by the Deutsche Forschungsgemeinschaft (SFB 51). Supplemcntum 159 1 1 

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TRF-stimulation-test in hypothalamic and pituitary diseases, and in autonomous adenoma of the thyroid

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