Horbach, E.

Otte, M.

Poschmann, A.

Schwarting, K.

Scriba, Peter Christian

Stöcker, W.

English

Universität München: Elektronischen Publikationen

Immunobiology 
Zeitschrift für Immunitätsforschung 
Editors 
E. D . ALBERT, München • D . BlTTER-SUERMANN, Hannover • M . P. DlERICH, Innsbruck • 
M . F E L D M A N N , London • S. H . E. K A U F M A N N , Ulm • E. K L E I N , Stockholm • W. K Ö H L E R , 
Jena • U . KOSZINOWSKI, U l m • P. KRAMMER, Heidelberg • M . L O O S , Mainz • T. LUGER, Wien • 
L . J. O L D , New York • J. J. OPPENHEIM, Frederick • H . H . PETER, Freiburg • K. PFIZENMAIER, 
Göttingen • M . RÖLLINGHOFF, Erlangen • D . SCHENDEL, München • V. SCHIRRMACHER, 
Heidelberg • C. SORG, Münster • R. VAN FURTH, Leiden • H . WAGNER, U l m • 
H . WEKERLE, Würzburg • G. W l C K , Innsbruck • R. ZlNKERNAGEL, Zürich 
Editor-in-Chief 
D. GEMSA, Marburg 
Editorial Advisory Board 
R. A V E R D U N K , Berlin • J . F. B A C H , Paris • H . B A L N E R , Rijswijk • R. B E N N E R , Rotterdam • 
H . v. BOEHMER, Basel • G. BONNARD, Bern • D . G. BRAUN, Basel • V. BRAUN, Tübingen • 
J. BROSTOFF, London • A. COUTINHO, Paris • T. DIAMANTSTEIN, Berlin • W. D R Ö G E , 
Heidelberg • P. DUKOR, Basel • P. ERB, Basel • H . - D . FLAD, Borstel • O. GÖTZE, Göttingen • 
E. GÜNTHER, Göttingen • U . H A D D I N G , Mainz • H . H A H N , Berlin • K. H Ä L A , Innsbruck • 
G. J. H Ä M M E R L I N G , Heidelberg • K. U . H A R T M A N N , Marburg • H . ZUR H A U S E N , Heidelberg • 
M . HESS, Bern • J. K A L D E N , Erlangen • B. K I N D R E D , Tübingen • T. J. K I N D T , New York • 
U . KOSZINOWSKI, Tübingen • E . KOWNATZKI , Freiburg • P. KRAMMER, Heidelberg • 
W . LEIBOLD, Hannover • K. LENNERT, Kiel • F. L I L L Y , New York • J. L l N D E M A N N , Zürich • 
E. MACHER, Münster • H . METZGER, Bethesda • V. TER MEULEN, Würzburg • H . J. MÜLLER-
EBERHARD, La Jolla • W. MÜLLER-RUCHHOLTZ, Kiel • H . PETERS, Göttingen • E. PlCK, Tel 
Aviv • O. PROKOP, Berlin • M . Q U A S T E L , Beer Sheva • J. P. REVILLARD, Lyon • E. P. RlEBER, 
München • E. RÜDE, Mainz • E. SCHÖPF, Freiburg • H . G. S C H W I C K , Marburg • K. SETHI , 
Heidelberg • G. SUNSHINE, London • N . T A L A L , San Francisco • G. T I L L , Ann Arbor • 
G. U H L E N B R U C H Köln • M . W A G N E R , Jena • H . W E K E R L E , Würzburg • P. W E R N E T , 
Tübingen 
Gustav Fischer Verlag • Stuttgart • New York • 1989 
Contents Volume 178 • 1989 
Original Papers 
BREEL, M . , J. L A M A N , and G . K R A A L : Murine Hybr id Cell Lines Expressing the N L D C -
145 Dendritic Cell Determinant 167 
CAI.I .FAVAERT, D . M . , P. MEYERS, J. H I E R N A U X , and G . R A D C L I F F : Kinetics of Cellular 
Cytotoxicity Mediated by Cloned Cytotoxic T Lymphocytes 203 
D A B R O W S K I , M . I . , H . GALKOWSKA, and W. L. OLSZEWSKI: Functional Characteristics of 
Veiled Cells from Canine Prenodal Lymph 316 
D E G R O O T , C . J . A . , C . D . DlJKSTRA, and T . S M I N I A : Discrimination between Different 
Types of Neuroglial Cells in Rat Central Nervous System Using Combined Immuno-
and Enzyme-Histochemical Methods 177 
G I A N E L L A , G . , E . PELOSI-TESTA, P. C A R L I N I , D . HABETSWALLNER, E . M O N T E S O R O , A . 
C A M A G N A , V . C A L Z I N I , E . M . RUGGERI, M . G . A R E N A , R . M A S C I U L L I , D . B U L G A R I N I , 
P. S A M O G G I A , S. D I F O L C O , G . PETRILLO, M . G . SOFRA, G . B O C C O L I , G . MASTROBE-
R A R D I N O , F. CALABRESI, U . TESTA, and C . PESCHLE: Fluctuations of Plasma ß2-
Microglobulin, Soluble Interleukin 2 Receptor and Interferon-Gamma Concentra-
tions after Adoptive Immunotherapy with High-Dose Interleukin 2 and Lymphokine-
Activated Killer Cells . . . 305 
JONES, C. M . , C. A . PRINCE, and R . K . S H A D D U C K : Characterization of a Human 
Monocyte Cytotoxicity-Inducing Factor (MCF) 229 
K L A S E N , I . S., J. P. DE J O N G , J . S. A . V O E R M A N , R . M . T . L A D E S T E I N , P. J . M . L E E N E N , 
and R . B E N N E R : Murine Macrophage Cell Line AP284 Presents Antigen to Cloned 
MT4 + , Ly t -2" T Cells in vitro and in vivo 261 
K O B A Y A S H I , K . , C. A L L R E D , and T . Y O S H I D A : Suppression of Interleukin 2 Production 
by Sera Obtained from Hypersensitivity Granuloma-Bearing Mice wi th Defective T 
Cell-Mediated Immune Responses 329 
LESER, H . - G . , W. G E R O K , D . GEMSA, and S. H . E . K A U F M A N N : Increased Prostaglandin 
E Release and Tumor Cytostasis by Resident Kupffer Cells during Listeria monocyto-
genes Infection 224 
L E U , R. W., J . A . R U M M A G E , and M . J . H O R N : Characterization of Murine Macrophage 
Fc Receptor-Dependent Phagocytosis and Antibody-Dependent Cellular Cytotoxic-
ity during in vitro Culture with Interferons-Gamma, Alpha/Beta and/or Fetal Bovine 
Scrum 340 
M A I . I C K , A. P., A. D. Y U R O C H K O , C. j . BURGER, D. ASKEW, and K. D . ELGERT: Tumor-
Induced Variations in a High Molecular Weight Inhibitory Monokine 361 
M A Y U M I , H . , and R. A . G O O D : The Necessity of Both Allogeneic Antigens and Stem 
Cells for Cyclophosphamide-Induced Skin Allograft Tolerance in Mice 287 
M I L L E R , S. C , and L . PoiRlER: Characteristics of Cells Mediating Spontaneous Resist-
ance to Bone Marrow Allografts 191 
M O L E N D I J K , A . , L . M . HUSSAARTS-ODIJK, R. J . H . L . M . V A N G U R P , H . DE RUITER, and 
R. B E N N E R : Suppression of Antigraft Immunity by Preimmunization. V. Characteri-
zation of Suppressor T Memory Cells 351 
SCHNEIDER, C. H . , and H . R o i X I : Potency of Hexavalent and Decavalent Anaphylacto-
gens: Affinity Enhancement by Epitope Clusters 250 
S H I M I Z U , T . , M . S. M A R T I N , H . PELLETIER, P. L A G A D E C , and F. M A R T I N : Effects of 
Cyclosporin A on Progressive and Regressive Tumors Induced by Two Cancer Lines 
Derived from a Single Colon Carcinoma Chemically Induced in the Rat 401 
SIEBER, G . , J . V. T E I C H M A N N , B. M Ö L L E R , and U . H O P F : Abnormalities of B Cell 
Activation and Immunoregulation in Patients with Chronic Inflammatory Liver 
Diseases 215 
464 • Contents Volume 
U D O N O , H . , K . K U R I B A Y A S H I , T . FUJITA, M . M I E N O , H . S H I K U , and E . N A K A Y A M A : 
Proliferative Response of Cloned C T L Line 10B-5 upon Stimulation with Soluble 
Clonotypic Monoclonal Antibody and its Blocking by Ant i -Ly t -2 Antibody 390 
Y O S H I O K A , K . , A . Fuji , H . T A H A R A , M . A R A O , and S. K A K U M U : Recombinant Human 
Interleukin l a is Cytotoxic for and Increases Surface Expression of H L A - A , B , C 
Antigens of a Human Hepatoma Cell Line, PLC/PRF/5 380 
Y U R O C H K O , A . D. , R. H . PYLE, and K . D . ELGERT: Changes in Macrophage Populations: 
Phenotypic Differences between Normal and Tumor-Bearing Host Macrophages . . . 416 
Rapid Communications 
G Ü N T H E R , C , M . R ö L L I N G H O F F , and H . U . BEUSCHER: Proteolysis of the Native Murine 
I L l ß Precursor is Required to Generate I L l ß Bioactivity 436 
H A U K E , G., M . H A D A M , J . T . EPPLEN, M . G A H R , A. H O L L M A N N , and H . H . PETER: 
Predominant Immunoglobulin Gene Rearrangements in Two Patients with 
Immunodeficiency: Restricted Use of V Gene Segments and D N A Hypermethylation . 449 
J O H N S O N , J . P., B. G. STADE, U . H U P K E , B. H O L Z M A N N , and G. R I E T H M Ü L L E R : The 
Melanoma Progression-Associated Antigen P3.58 is Identical to the Intercellular 
Adhesion Molecule, I C A M - 1 275 
Abstracts: X X t h Meeting of the Society of Immunology, October 1988, Düsseldorf . .1-166 
140 • X X t h Meeting of the Society of Immunology 
Klinik für Innere Medizin und Institut für Immunologie und Transfusionsmedizin der 
Medizinischen Universität Lübeck, FRG 
K.10 Monoclonal antibodies against the Crohn's disease-involved 
pancreatic autoantigen 
E . H O R B A C H , W . STÖCKER, M . O T T E , P. C. SCRIBA, K. S C H W A R T I N G , and A. P O S C H M A N N 
In previous studies, it was possible to show that patients with Crohn's disease (CD) exhibit 
an autoimmunity against a macromolecular antigen contained in pancreatic juice ( 1 ) . Some 
observations suggest that autoimmunity to exocrine pancreas is of essential importance in the 
pathogenesis of C D . To get means for further investigation, monoclonal antibodies were 
produced against the CD-involved autoantigen. 
To enrich the autoantigen, corresponding to the CD-patients' pancreatic autoantibodies, 
human pancreatic tissue was homogenized and centrifuged ( 3 0 , 0 0 0 x g). The fat-free superna-
tant was subjected to size exclusion chromatography. The antigen-containing fraction was 
identified by a neutralization test combined with the immunofluorescent procedure used for 
detection of pancreatic autoantibodies ( 2 ) . The antigen was precipitated by 2 5 / 5 0 percent 
ammoniumsulfate, resolved in phosphate buffered saline to appropriate concentration and 
used for immunization of female BALB/c mice according to ( 3 ) . Splenocytes of immunized 
mice were fused wi th myeloma cell line P 3 - X 6 3 - A g 8 - U l in a relation of 5:1 in the presence of 
X X t h Meeting of the Society of Immunology • 141 
4 0 percent polyethylenglycol (Merck, Darmstadt) and 1 5 percent dimethyl sulfoxide, follow-
ing standard techniques. Hybrids were raised in H A T medium containing 2 0 percent FCS and 
repeatedly cloned by limited dilution. Positive clones were identified by indirect immuno-
fluorescence, using frozen sections of human pancreas which were incubated wi th undiluted 
supernatants and, in the second step, with FITC-labelled antimouse IgG. 
Out of 5 4 0 clones 1 2 were selected which produced an antibody reacting with exocrine 
pancreas and exhibiting a similar pattern as human autoantibodies of patients wi th C D . Two of 
these ( M A A - l 7 / 4 - 1 , M A F - 2 1 / 2 - 1 0 ) remained stable in producing antibodies which gave a 
strong reaction wi th exocrine pancreas. Further characteristics of these monoclonal antibodies 
are described in detail. 
The established monoclonal antibodies against the CD-involved autoantigen might be useful 
as reagents to purify and identify the CD-involved autoantigen or to localize it in biopsy tissue 
of patients with chronic inflammatory bowel disease thus enabling a better diagnosis of C D . 
1. STÖCKER, W . , M . O T T E , S. U L R I C H , D . N O R M A N , H . F I N K B E I N E R , K . STÖCKER, G. 
JANTSCHEK, and P. C. SCRIBA. 1987 . Scand. J . Gastroenterol. 2 2 , Suppl. 1 3 9 : 4 1 - 5 2 . 
2. F I N K B E I N E R , H . , S. B O C K , U . BURMESTER, D . G R A G E , U . R E D D I G , D . STRUVE, M . O T T E , 
and W . STÖCKER. 1985. Immunobiol. 1 7 0 : 2 0 - 2 1 . 
3. S T Ä H L I , C , T. S T A E H L I N , V. M I G G I A N O , j . S C H M I D T , and P. H Ä R I N G . 1980 . J. Immunol. 
Mcth. 3 2 : 2 9 7 - 3 0 4 . 


Monoclonal antibodies against the Crohn´s disease - involved pancreatic autoantigen

Open Access LMU

Immunobiology Zeitschrift für Immunitätsforschung Editors E. D . ALBERT, München • D . BlTTER-SUERMANN, Hannover • M . P. DlERICH, Innsbruck • M . F E L D M A N N , London • S. H . E. K A U F M A N N , Ulm • E. K L E I N , Stockholm • W. K Ö H L E R , Jena • U . KOSZINOWSKI, U l m • P. KRAMMER, Heidelberg • M . L O O S , Mainz • T. LUGER, Wien • L . J. O L D , New York • J. J. OPPENHEIM, Frederick • H . H . PETER, Freiburg • K. PFIZENMAIER, Göttingen • M . RÖLLINGHOFF, Erlangen • D . SCHENDEL, München • V. SCHIRRMACHER, Heidelberg • C. SORG, Münster • R. VAN FURTH, Leiden • H . WAGNER, U l m • H . WEKERLE, Würzburg • G. W l C K , Innsbruck • R. ZlNKERNAGEL, Zürich Editor-in-Chief D. GEMSA, Marburg Editorial Advisory Board R. A V E R D U N K , Berlin • J . F. B A C H , Paris • H . B A L N E R , Rijswijk • R. B E N N E R , Rotterdam • H . v. BOEHMER, Basel • G. BONNARD, Bern • D . G. BRAUN, Basel • V. BRAUN, Tübingen • J. BROSTOFF, London • A. COUTINHO, Paris • T. DIAMANTSTEIN, Berlin • W. D R Ö G E , Heidelberg • P. DUKOR, Basel • P. ERB, Basel • H . - D . FLAD, Borstel • O. GÖTZE, Göttingen • E. GÜNTHER, Göttingen • U . H A D D I N G , Mainz • H . H A H N , Berlin • K. H Ä L A , Innsbruck • G. J. H Ä M M E R L I N G , Heidelberg • K. U . H A R T M A N N , Marburg • H . ZUR H A U S E N , Heidelberg • M . HESS, Bern • J. K A L D E N , Erlangen • B. K I N D R E D , Tübingen • T. J. K I N D T , New York • U . KOSZINOWSKI, Tübingen • E . KOWNATZKI , Freiburg • P. KRAMMER, Heidelberg • W . LEIBOLD, Hannover • K. LENNERT, Kiel • F. L I L L Y , New York • J. L l N D E M A N N , Zürich • E. MACHER, Münster • H . METZGER, Bethesda • V. TER MEULEN, Würzburg • H . J. MÜLLER-EBERHARD, La Jolla • W. MÜLLER-RUCHHOLTZ, Kiel • H . PETERS, Göttingen • E. PlCK, Tel Aviv • O. PROKOP, Berlin • M . Q U A S T E L , Beer Sheva • J. P. REVILLARD, Lyon • E. P. RlEBER, München • E. RÜDE, Mainz • E. SCHÖPF, Freiburg • H . G. S C H W I C K , Marburg • K. SETHI , Heidelberg • G. SUNSHINE, London • N . T A L A L , San Francisco • G. T I L L , Ann Arbor • G. U H L E N B R U C H Köln • M . W A G N E R , Jena • H . W E K E R L E , Würzburg • P. W E R N E T , Tübingen Gustav Fischer Verlag • Stuttgart • New York • 1989 Contents Volume 178 • 1989 Original Papers BREEL, M . , J. L A M A N , and G . K R A A L : Murine Hybr id Cell Lines Expressing the N L D C -145 Dendritic Cell Determinant 167 CAI.I .FAVAERT, D . M . , P. MEYERS, J. H I E R N A U X , and G . R A D C L I F F : Kinetics of Cellular Cytotoxicity Mediated by Cloned Cytotoxic T Lymphocytes 203 D A B R O W S K I , M . I . , H . GALKOWSKA, and W. L. OLSZEWSKI: Functional Characteristics of Veiled Cells from Canine Prenodal Lymph 316 D E G R O O T , C . J . A . , C . D . DlJKSTRA, and T . S M I N I A : Discrimination between Different Types of Neuroglial Cells in Rat Central Nervous System Using Combined Immuno-and Enzyme-Histochemical Methods 177 G I A N E L L A , G . , E . PELOSI-TESTA, P. C A R L I N I , D . HABETSWALLNER, E . M O N T E S O R O , A . C A M A G N A , V . C A L Z I N I , E . M . RUGGERI, M . G . A R E N A , R . M A S C I U L L I , D . B U L G A R I N I , P. S A M O G G I A , S. D I F O L C O , G . PETRILLO, M . G . SOFRA, G . B O C C O L I , G . MASTROBE-R A R D I N O , F. CALABRESI, U . TESTA, and C . PESCHLE: Fluctuations of Plasma ß2-Microglobulin, Soluble Interleukin 2 Receptor and Interferon-Gamma Concentra-tions after Adoptive Immunotherapy with High-Dose Interleukin 2 and Lymphokine-Activated Killer Cells . . . 305 JONES, C. M . , C. A . PRINCE, and R . K . S H A D D U C K : Characterization of a Human Monocyte Cytotoxicity-Inducing Factor (MCF) 229 K L A S E N , I . S., J. P. DE J O N G , J . S. A . V O E R M A N , R . M . T . L A D E S T E I N , P. J . M . L E E N E N , and R . B E N N E R : Murine Macrophage Cell Line AP284 Presents Antigen to Cloned MT4 + , Ly t -2" T Cells in vitro and in vivo 261 K O B A Y A S H I , K . , C. A L L R E D , and T . Y O S H I D A : Suppression of Interleukin 2 Production by Sera Obtained from Hypersensitivity Granuloma-Bearing Mice wi th Defective T Cell-Mediated Immune Responses 329 LESER, H . - G . , W. G E R O K , D . GEMSA, and S. H . E . K A U F M A N N : Increased Prostaglandin E Release and Tumor Cytostasis by Resident Kupffer Cells during Listeria monocyto-genes Infection 224 L E U , R. W., J . A . R U M M A G E , and M . J . H O R N : Characterization of Murine Macrophage Fc Receptor-Dependent Phagocytosis and Antibody-Dependent Cellular Cytotoxic-ity during in vitro Culture with Interferons-Gamma, Alpha/Beta and/or Fetal Bovine Scrum 340 M A I . I C K , A. P., A. D. Y U R O C H K O , C. j . BURGER, D. ASKEW, and K. D . ELGERT: Tumor-Induced Variations in a High Molecular Weight Inhibitory Monokine 361 M A Y U M I , H . , and R. A . G O O D : The Necessity of Both Allogeneic Antigens and Stem Cells for Cyclophosphamide-Induced Skin Allograft Tolerance in Mice 287 M I L L E R , S. C , and L . PoiRlER: Characteristics of Cells Mediating Spontaneous Resist-ance to Bone Marrow Allografts 191 M O L E N D I J K , A . , L . M . HUSSAARTS-ODIJK, R. J . H . L . M . V A N G U R P , H . DE RUITER, and R. B E N N E R : Suppression of Antigraft Immunity by Preimmunization. V. Characteri-zation of Suppressor T Memory Cells 351 SCHNEIDER, C. H . , and H . R o i X I : Potency of Hexavalent and Decavalent Anaphylacto-gens: Affinity Enhancement by Epitope Clusters 250 S H I M I Z U , T . , M . S. M A R T I N , H . PELLETIER, P. L A G A D E C , and F. M A R T I N : Effects of Cyclosporin A on Progressive and Regressive Tumors Induced by Two Cancer Lines Derived from a Single Colon Carcinoma Chemically Induced in the Rat 401 SIEBER, G . , J . V. T E I C H M A N N , B. M Ö L L E R , and U . H O P F : Abnormalities of B Cell Activation and Immunoregulation in Patients with Chronic Inflammatory Liver Diseases 215 464 • Contents Volume U D O N O , H . , K . K U R I B A Y A S H I , T . FUJITA, M . M I E N O , H . S H I K U , and E . N A K A Y A M A : Proliferative Response of Cloned C T L Line 10B-5 upon Stimulation with Soluble Clonotypic Monoclonal Antibody and its Blocking by Ant i -Ly t -2 Antibody 390 Y O S H I O K A , K . , A . Fuji , H . T A H A R A , M . A R A O , and S. K A K U M U : Recombinant Human Interleukin l a is Cytotoxic for and Increases Surface Expression of H L A - A , B , C Antigens of a Human Hepatoma Cell Line, PLC/PRF/5 380 Y U R O C H K O , A . D. , R. H . PYLE, and K . D . ELGERT: Changes in Macrophage Populations: Phenotypic Differences between Normal and Tumor-Bearing Host Macrophages . . . 416 Rapid Communications G Ü N T H E R , C , M . R ö L L I N G H O F F , and H . U . BEUSCHER: Proteolysis of the Native Murine I L l ß Precursor is Required to Generate I L l ß Bioactivity 436 H A U K E , G., M . H A D A M , J . T . EPPLEN, M . G A H R , A. H O L L M A N N , and H . H . PETER: Predominant Immunoglobulin Gene Rearrangements in Two Patients with Immunodeficiency: Restricted Use of V Gene Segments and D N A Hypermethylation . 449 J O H N S O N , J . P., B. G. STADE, U . H U P K E , B. H O L Z M A N N , and G. R I E T H M Ü L L E R : The Melanoma Progression-Associated Antigen P3.58 is Identical to the Intercellular Adhesion Molecule, I C A M - 1 275 Abstracts: X X t h Meeting of the Society of Immunology, October 1988, Düsseldorf . .1-166 140 • X X t h Meeting of the Society of Immunology Klinik für Innere Medizin und Institut für Immunologie und Transfusionsmedizin der Medizinischen Universität Lübeck, FRG K.10 Monoclonal antibodies against the Crohn's disease-involved pancreatic autoantigen E . H O R B A C H , W . STÖCKER, M . O T T E , P. C. SCRIBA, K. S C H W A R T I N G , and A. P O S C H M A N N In previous studies, it was possible to show that patients with Crohn's disease (CD) exhibit an autoimmunity against a macromolecular antigen contained in pancreatic juice ( 1 ) . Some observations suggest that autoimmunity to exocrine pancreas is of essential importance in the pathogenesis of C D . To get means for further investigation, monoclonal antibodies were produced against the CD-involved autoantigen. To enrich the autoantigen, corresponding to the CD-patients' pancreatic autoantibodies, human pancreatic tissue was homogenized and centrifuged ( 3 0 , 0 0 0 x g). The fat-free superna-tant was subjected to size exclusion chromatography. The antigen-containing fraction was identified by a neutralization test combined with the immunofluorescent procedure used for detection of pancreatic autoantibodies ( 2 ) . The antigen was precipitated by 2 5 / 5 0 percent ammoniumsulfate, resolved in phosphate buffered saline to appropriate concentration and used for immunization of female BALB/c mice according to ( 3 ) . Splenocytes of immunized mice were fused wi th myeloma cell line P 3 - X 6 3 - A g 8 - U l in a relation of 5:1 in the presence of X X t h Meeting of the Society of Immunology • 141 4 0 percent polyethylenglycol (Merck, Darmstadt) and 1 5 percent dimethyl sulfoxide, follow-ing standard techniques. Hybrids were raised in H A T medium containing 2 0 percent FCS and repeatedly cloned by limited dilution. Positive clones were identified by indirect immuno-fluorescence, using frozen sections of human pancreas which were incubated wi th undiluted supernatants and, in the second step, with FITC-labelled antimouse IgG. Out of 5 4 0 clones 1 2 were selected which produced an antibody reacting with exocrine pancreas and exhibiting a similar pattern as human autoantibodies of patients wi th C D . Two of these ( M A A - l 7 / 4 - 1 , M A F - 2 1 / 2 - 1 0 ) remained stable in producing antibodies which gave a strong reaction wi th exocrine pancreas. Further characteristics of these monoclonal antibodies are described in detail. The established monoclonal antibodies against the CD-involved autoantigen might be useful as reagents to purify and identify the CD-involved autoantigen or to localize it in biopsy tissue of patients with chronic inflammatory bowel disease thus enabling a better diagnosis of C D . 1. STÖCKER, W . , M . O T T E , S. U L R I C H , D . N O R M A N , H . F I N K B E I N E R , K . STÖCKER, G. JANTSCHEK, and P. C. SCRIBA. 1987 . Scand. J . Gastroenterol. 2 2 , Suppl. 1 3 9 : 4 1 - 5 2 . 2. F I N K B E I N E R , H . , S. B O C K , U . BURMESTER, D . G R A G E , U . R E D D I G , D . STRUVE, M . O T T E , and W . STÖCKER. 1985. Immunobiol. 1 7 0 : 2 0 - 2 1 . 3. S T Ä H L I , C , T. S T A E H L I N , V. M I G G I A N O , j . S C H M I D T , and P. H Ä R I N G . 1980 . J. Immunol. Mcth. 3 2 : 2 9 7 - 3 0 4 . 

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Monoclonal antibodies against the Crohn´s disease - involved pancreatic autoantigen

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