Figure S3. T cell differentiation subsets formed during in vitro stimulation with ÎąCD3/CD80 stimulation. Negatively-selected healthy donor T cells were cultured with 5Â Îźg/mL ÎąCD3 and the indicated concentration of CD80. T cell differentiation subsets were quantified following four days of culture. A, Flow plot of gating strategy to identify the indicated T cell differentiation subsets. B, Flow plots of CD4 (top) and CD8 (bottom) T cells cultured under the indicated conditions. (PDF 3995 kb