<p>DNA channel showing nuclei (A), CellMaskDeepRed channel showing membrane (B), and GFP channel showing beta-actin (C) at the center (left) and edge (right) of the hiPSC colony. (D) Various measurements obtained from the samples are shown; note that cells touching the edge of each image are excluded from this analysis. The underlying measurements may be downloaded as <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005970#pbio.2005970.s013" target="_blank">S2 File</a>. <i>Images are from the Allen Institute for Cell Science</i>, <i>Seattle</i>, <i>and are available from the Broad Bioimage Benchmark Collection (<a href="https://data.broadinstitute.org/bbbc/BBBC034/" target="_blank">https://data.broadinstitute.org/bbbc/BBBC034/</a>)</i>. 3D, three-dimensional; GFP, green fluorescent protein; hiPSC, human induced pluripotent stem cell.</p
