Video 2: Comparison of image volumes acquired with LSTM in 1-AS and 2-AS modes.

Abstract

<div><p>The 3D rendering visualizes the image stacks acquired from the same sample (human brain section stained with DAPI) with LSTM in 1-Axis Scan (1-AS) and simultaneous 2-Axes Scan (2-AS) mode.</p><p><br></p> <p>This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.</p></div><div><br></div><div>LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.</div><div><br></div><div>View the full collection of related videos at: </div><p><a href="https://doi.org/10.6084/m9.figshare.c.4072160">https://doi.org/10.6084/m9.figshare.c.4072160</a></p