Figure S11. G85R-SOD1:YFP expression in spinal cord is 2-fold higher than forebrain in G85R-SOD1:YFP heterozygous mice. Forebrain and spinal cord tissue were extracted and 20 μg of protein was used for immunoblot analysis of SOD1 levels, using an antibody specific for human SOD1 (hSOD1) (a). Each lane represents an individual animal (n = 3). Graph represents densitometric quantification of hSOD1 levels normalized to GAPDH (b). Statistical analysis was conducted using GraphPad Prism (version 7.0 h) Error bars show mean ± S.D.; unpaired T-test. A.U.; arbitrary units. (TIF 248 kb
