Additional file 2: of A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice

Abstract

Figure S2. The SFAs in HFDs disrupted feeding patterns by increasing light cycle intake. (a–c) Mice were fed one of two diets high in saturated fatty acids (SFAs); the HFD60 contained higher amounts of C16:0 and C18:0 (n = 6, black circles with solid line) than the HFD32 (n = 8, white squares with dashed line). After acclimation to FDAMS with NC, mice were fed the HFDs for 5 days. White and black bars on the X-axis correspond to the light and dark cycles, respectively. (a-b) Hourly caloric intake (1 kcal = 4.186 kJ) before (Pre) and after the diet switch from NC to (a) HFD60 or (b) HFD32. (c) The light cycle intake expressed as a percentage of the 24-h intake. Black circles with solid line: the HFD60 group; white squares with dashed line: the HFD32 group. Data are the means ± s.e.m. Statistical significance was determined with the Student’s paired t-test for comparisons to pre-diet values for each group, in c; *P < 0.05. Abbreviations: NC, normal chow; HFD, high-fat diet; FDAMS, feeding drinking, and activity monitoring system. (TIF 226 kb