Additional file 16: of STARD13-correlated ceRNA network-directed inhibition on YAP/TAZ activity suppresses stemness of breast cancer via co-regulating Hippo and Rho-GTPase/F-actin signaling

Abstract

Figure S11. Depletion of STARD13-correlated ceRNAs dampens the response of breast cancer cells to doxorubicin. (A) IC50 curves of MCF-7 cells with STARD13-correlated ceRNA knockdown and were fitted with a nonlinear regression model and were presented as log (Doxorubicin) vs cell viability. (B) Western blot assay of lysates from MCF-7 cells with STARD13-correlated ceRNA knockdown. (C) Images of tumors harvested when STARD13 3′UTR stable overexpression cells were planted and followed by doxorubicin treatment or not. The weight of tumors harvested in (C) was monitored. (D) The weight of mice depicted in (C) was monitored. (E) The volume of tumors harvested in (C) was monitored. (G) Confocal images of MCF-7 cells described in (B) with doxorubicin treatment. Depletion of STARD13-correlated ceRNAs impaired the cellular retention of doxorubicin. (F) Western blot assay of lysates from MCF-7 cells with STARD13-correlated ceRNA knockdown plus si-Dicer or not. (TIF 3999 kb