Additional file 2 of The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling

Abstract

Figure S2 The localization of YFP-TGAT is hardly affected by the treatment with 2-BP. Confocal images of cells expressing YFP-TGAT and GAP43-CFP. The GAP43 fusion is used to monitor the palmitoylation status of proteins in cells. In untreated cells GAP43 is located at the plasma membrane due to palmitoylation. Overnight treatment with 60 ΟM 2-BP inhibits palmitoylation of GAP43, which is reflected by the cytoplasmic localization. In contrast to GAP43, the localization of YFP-TGAT is hardly affected by the treatment with 2-BP. (PNG 963 kb