<p><b>(A)</b> Bands represent transcript levels determined by semiquantitative RT-PCR. Genotypes are labeled above, target transcripts at the left, and germ line phenotypes below; <i>act-1</i> is a loading control. For each lane, RNA was isolated from batches of five worms (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005069#sec017" target="_blank">Materials and methods</a>). The alleles were <i>him-5(e1490)</i>, <i>fem-3(q96)</i>, <i>fem-1(hc17</i>ts<i>)</i>, <i>fog-1(q253</i>ts<i>)</i>, and <i>glp-4(bn2</i>ts<i>)</i>. (B) Diagram illustrating the insertion site of the GFP coding sequence into the endogenous <i>zipt-7</i>.<i>1</i> locus to generate the <i>zipt-7</i>.<i>1(ibp18)</i> strain. (C) Sets of photomicrographs from two extruded male gonads. The location of the images in the gonad arm is indicated with a red box on the cartoon; distal is up and proximal to the right. The bright-field images show cell morphology (left), the antibody stain shows the expression of GFP::ZIPT-7.1 (middle), and the DAPI stain shows cell nuclei (right). Genotypes were <i>him-5(e1490)</i> and <i>him-5(e1490) zipt-7</i>.<i>1(ibp18)</i>. Scale bar is 10 μm. (D) Reproductive defects caused by <i>zipt-7</i>.<i>1</i> RNAi treatment. The F<sub>1</sub> progeny of mothers injected with dsRNA were each scored for whether they laid eggs, oocytes, or both. Dotted lines show the average percentage for each phenotype and sum to 100%, the size of the boxes indicates 95% confidence limits, and the color indicates the phenotype according to the key. The <i>rrf-1</i> genotype and RNAi treatment are indicated at the bottom (<i>N</i> = 200, 248, and 315, left to right). Wild-type animals are susceptible to RNAi in germ cells and most somatic cells, whereas the <i>rrf-1</i> mutant animals are susceptible to RNAi in germ cells but resistant in most of the somatic cells [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005069#pbio.2005069.ref017" target="_blank">17</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005069#pbio.2005069.ref019" target="_blank">19</a>], as indicated in the diagram. The individual numerical values for panel D can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005069#pbio.2005069.s008" target="_blank">S1 Data</a>. DIC, differential interference contrast; dsRNA, double-stranded RNA; GFP, green fluorescent protein; Oo, oocyte; Sp, sperm; RNAi, RNA interference; RT-PCR, Reverse transcription polymerase chain reaction.</p
