Figure S1. Location of gene-specific primers and PCR amplification of TaPSTOL genomic regions on DNA extracted from nullisomic (N) / tetrasomic (T) wheat lines. The PCR amplicons correspond to a promoter region (PCR1, 1458Â bp), a promoter and coding region (PCR2, 553Â bp), and a coding region (PCR3, 870Â bp), to demonstrate that TaPSTOL is only present on chromosome 5A. (DOCX 63 kb