Figure S4. OSM is highly expressed in orthotopic 4T1.2 primary mammary tumors in female BALB/c mice. Histology using H&E confirmed the presence of a large primary mammary tumor (T) 32Â days after 4T1.2 mouse mammary tumor cell injection into the fourth mammary fat pad of female BALB/c mice. High OSM expression is seen in the tumor, as is background expression in the normal breast connective tissue (CT). OSM expression is shown to be highest in the invasive edge of the tumor (T) closest to the normal breast connective tissue (CT). Control slides with no primary OSM antibody show low background staining. (PPTX 315Â kb
