<p>Two new polyketide metabolites, the 12-membered macrolides 4-hydroxy-12-methyloxacyclododecane-2,5,6-trione (<b>1</b>) and 12-methyloxacyclododecane-2,5,6-trione (<b>2</b>), were isolated from the endophytic fungal strain <i>Cladosprium colocasiae</i> A801 of the plant <i>Callistemon viminalis</i>, together with five known derivatives<i>.</i> Their structures were fully characterized by means of detailed spectroscopic analysis for new structures, and in comparison with published data for known compounds. The antibacterial, cytotoxic, and α-glucosidase inhibitory activities of the new compounds <b>1</b> and <b>2</b> were evaluated.</p
