Additional file 5: of COP9 signalosome subunit 5A affects phenylpropanoid metabolism, trichome formation and transcription of key genes of a regulatory tri-protein complex in Arabidopsis

Abstract

Figure S2. Molecular characterization of sk372. (A) Southern blot; (B) homozygous determination of sk372. (D) CSN5a truncation analysis using cDNA as a template. Lanes 1 and 2, full length (938 bp) of CSN5a in WT and sk372, respectively; Lanes 3 and 4, amplified fragment from 162 bp to 886 bp in WT and sk372; Lanes 5 and 6, amplified fragment starting from bp 1034 to 3′ end in WT and sk372.The presence of transgene GL3 in the different independent lines of transgenic sk372. M, 1 kb plus DNA marker (Invitrogen); T1-T8, different independent lines of transgenic plants; WT, wild type control. (TIF 7690 kb

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