Testing FAA in forebrain and pan neuronal deletion mutants of Sirt1.

Abstract

<p>(A) Mean body weights for <i>CamKII-Cre; Sirt1</i>^{<i>loxP/loxP</i>} and <i>Sirt1</i>^{<i>loxP/loxP</i>} littermate controls. CR diets began on Day 0. Mean high activity data for home cage behaviors at (B) Day -7 (n = 8 WT and n = 9 for KO), (C) Day 14 of CR (n = 6 WT and n = 9 KO), and (D) Day 28 (n = 7 WT and n = 8 KO) of CR are shown. (E) Mean total high activity, measured in seconds, over the entire 23.5h-24h video recording for <i>Sirt1</i>^{<i>loxP/loxP</i>} controls and CamKII-Cre;<i>Sirt1</i>^{<i>loxP/loxP</i>} (F) Mean fraction of normalized high activity in the 3h prior to mealtime; there were no differences between groups on all days. (G) Mean body weight for <i>Nestin-Cre; Sirt1</i>^{<i>loxP/loxP</i>} and <i>Sirt1</i>^{<i>loxP/loxP</i>} littermate controls. Mean high activity data for home cage behaviors at (H) Day -7 (n = 5 WT, n = 7 KO), (I) Day 14 (n = 4 WT, n = 4 KO) of CR, and (J) Day 28 (n = 5 WT, n = 7 KO) of CR are shown. (K) The amount of high activity in seconds for <i>Sirt1</i>^{<i>loxP/loxP</i>} and Nestin-Cre; <i>Sirt1</i>^{<i>loxP/loxP</i>} mice. On Day 7, the amount of total activity was greater for than <i>Nestin-Cre; Sirt1</i>^{<i>loxP/loxP</i>} compared to <i>Sirt1</i>^{<i>loxP/loxP</i>} controls (P = 0.0333). (L) Fraction of high activity measures between <i>Sirt1</i>^{<i>loxP/loxP</i>} and <i>Nestin-Cre; Sirt1</i>^{<i>loxP/loxP</i>} groups exhibit a greater amount of FAA in controls on Day 7 (P = 0.0333). For body weight data, statistical significance was determined using an unpaired T test. For behavioral data, statistical significance was determined using the Mann-Whitney Test. * denotes p<0.05.</p