<p>(A-D) Mean ± SEM of the geometric mean fluorescence intensity of activation marker expression on donor CD4 T cells harvested from spleens of recipient mice at week 1 (circles), week 2 (squares), or week 3 (triangles) post CD4 T cell transfer. Data is pooled from CD20-B<sup>MHCII</sup>xIgH<sup>MOG</sup> mice, UBC<sup>MHCII</sup> mice, and IAß<sup>b</sup>stop<sup>flox/flox</sup>xIgH<sup>MOG</sup> (Cre<sup>-</sup>) littermate control recipients from five independent experiments with n = 2–4 mice per time point evaluated. Significance tested by one way ANOVA with Tukey’s test for multiple comparisons. Geometric mean fluorescence intensity of (A) CD44, (B) CD62L, (C) CD25, and (D) CD69 expression on donor CD4 T cells. (E) Frequency of IFNγ-expressing donor CD4 T cells pre-transfer and following <i>in vivo</i> incubation for three weeks in MHCII-deficient hosts. Graph is representative of n = 5 independent experiments with at least two mice from each genotype. Donor T cells were harvested from the spleen. Significance tested by Kruskal-Wallis test and Dunn’s correction for multiple comparisons.</p
