Table S5. KEGG analysis of DEGs. (XLS 62Â kb). (XLS 78 kb

Bo-fan Yu (5476874)

Fang Yu (156838)

Ya-jie Pan (5476877)

Ying-chao Lin (73592)

Yuan-gang Zu (2134912)

Zhong-Hua Tang (516324)

BMC Genomics

Figure S2. The effect of ET on metabolic pathway genes. (DOC 286Â KB) (DOCX 285 kb

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Additional file 7: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Abstract Background The medicinal plant, Catharanthus roseus (C. roseus), accumulates a wide range of terpenoid indole alkaloids (TIAs). Ethylene (ET) and methyl-jasmonate (MeJA) were previously reported as effective elicitors for the production of various valuable secondary metabolites of C. roseus, while a few ET or MeJA induced transcriptomic research is yet reported on this species. In this study, the de-novo transcriptome assembly of C. roseus is performed by using the next-generation sequencing technology. Results The result shows that phenolic biosynthesis genes respond specifically to ET in leaves, monoterpenoid biosynthesis genes respond specifically to MeJA in roots. By screening the database, 23 ATP-binding cassette (ABC) transporter partial sequences are identified in C. roseus. On this basis, more than 80 key genes that encode key enzymes (namely TIA pathway, transcriptional factor (TF) and candidate ABC transporter) of alkaloid synthesis in TIA biosynthetic pathways are chosen to explore the integrative responses to ET and MeJA at the transcriptional level. Our data indicated that TIA accumulation is strictly regulated by the TF ethylene responsive factor (ERF) and bHLH iridoid synthesis 1 (BIS1). The heatmap, combined with principal component analysis (PCA) of C. roseus, shows that ERF co-expression with ABC2 and ABC8 specific expression in roots affect the root-specific accumulation of vinblastine in C. roseus. On the contrast, BIS1 activities follow a similar pattern of ABC3 and CrTPT2 specific expression in leaves, which affects the leaf-specific accumulation of vindoline in C. roseus. Conclusions Results presented above illustrate that ethylene has a stronger effect than MeJA on TIA induction at both transcriptional and metabolite level. Furthermore, meta-analysis reveals that ERF and BIS1 form a positive feedback loop connecting two ABC transporters respectively and are actively involved in TIAs responding to ET and MeJA in C. roseus

Ya-jie Pan

Ying-chao Lin

Bo-fan Yu

Yuan-gang Zu

Fang Yu

Zhong-Hua Tang

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Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Additional file 6: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Figure S1. Gene Ontology classification. (DOC 338Â KB) (DOCX 337 kb

Additional file 4: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Table S4. GO analysis of DEGs. (XLS 37Â kb). (XLSX 36 kb

Additional file 5: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Table S7. The expression of TIA-relative genes. (TIA pathway genes, TFs, ABC transporters) in response to ET or MeJA in different tissues by qRT-PCR. (XLS 60Â kb). (XLS 59 kb

Additional file 9: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Table S8. The FPKM value of 339 Candidate ABC Transporter Genes. (XLS 432Â kb). (XLS 432 kb

Additional file 10: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Table S6. GO analysis the genes of ET specific regulatory. (XLS 11Â kb). (XLSX 10 kb

Additional file 8: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

Table S1. Statistical summary of sequencing and assembly results. (XLS 21Â kb). (XLS 21 kb

Additional file 1: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

https://figshare.com/articles/Additional_file_5_of_Transcriptomics_comparison_reveals_the_diversity_of_ethylene_and_methyl-jasmonate_in_roles_of_TIA_metabolism_in_Catharanthus_roseus/6736001

Additional file 6: of Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus

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