Figure S3. Activation of HSF1 in C9ORF72-ALS, FTLD, and combined ALS/FTLD patients. (a) Quantitative real-time PCR (qRT-PCR) for HSF1 target genes in the cerebellum of sporadic and C9ORF72-associated disease (n = 56 C9ORF72-ALS/FTLD, n = 42 sporadic ALS/FTLD, n = 7 controls) (one-way ANOVA with Bonferonni post-hoc test for multiple comparisons * p < 0.05, ** p < 0.01, *** p < 0.001. Note, no significant changes were detected between the sporadic cases and controls. (b) Correlation of HSF1 levels and HSF1 target gene levels in the frontal cortex and cerebellum in C9ORF72-ALS/FTLD. Spearman’s R2 values are plotted for each target gene, error bars denote 95% confidence interval, p-value < 0.0001 in all cases. (PDF 209 kb
