Figure S4. Effect of MSCs on platelet activation using different agonists and pathway inhibitors. A, B Effect of 105 LA-MSCs/ml on platelet activation after stimulation with different agonists ADP, TRAP-6 and U46619 (n = 4). Expression of two different activation markers shown: A CD62P and B PAC-1 binding. *p < 0.05. C, D Effect of AK4 and indomethacin on platelet inhibition by 5 × 105 BM-MSCs/ml. Platelets stimulated with TRAP-6. x axis, PAC-1 fluorescence intensity; y, axis, platelet count. One of two experiments shown: C AK4 to block CD62P and D MSC preculture in indomethacin to block COX. (TIF 176 kb