Supplemental Figure 4. In cells depleted of CD147, collapsed L. monocytogenes membrane protrusions still contain ezrin while comet/rocket tails remain morphologically normal.

Abstract

<p>(A) HeLa cells were treated with non-targeting control (Ctrl) or CD147-targeted (knockdown [KD]) siRNA sequences, and whole-cell lysates were collected and probed for endogenous CD147 using mouse monoclonal CD147-targeting antibodies. Loading control with α-tubulin is shown below.</p> <p>(B) Ezrin immunolocalization (green) at actin-rich <i>L. monocytogenes</i> membrane protrusions (red) generated in cells treated with non-targeting control (top) or CD147-targeting (bottom) siRNA sequences. Ezrin is present within the core of wild-type as well as collapsed <i>L. monocytogenes</i> membrane protrusions. Open arrowheads indicate the location of <i>L. monocytogenes</i> bacteria (blue) within their respective membrane protrusion. Scale bar is 10 μm.</p> <p>(C) Cytosolic comet/rocket tails (solid arrowheads) appear morphologically normal in cells treated with CD147-targeting siRNA sequences. Open arrowheads indicate collapsed and/or contorted <i>L. monocytogenes </i>membrane protrusions. Scale bar is 10 μm.</p