<p><b>A</b>, NIH3T3 cells arrested in G<sub>0</sub>/G<sub>1</sub> (serum starvation), S-phase (double thymidine block) or G<sub>2</sub>/M (double thymidine block and nocodazole treatment) phase. Asynchronous cells shown as a control. Numbers on the panels indicate distribution of cells between cell cycle phases. Fitted peaks are: <i>Blue</i>, calculated G<sub>0</sub>/G<sub>1</sub> phase; <i>yellow</i>, S phase; <i>green</i>, G<sub>2</sub>/M phase. Cell cycle data were analyzed using FlowJo software. <b>B</b>, Western blot assay of ALDH1L1 in NIH3T3 cells arrested in indicated phase (20 μg of total cell lysate was loaded in each lane). Actin is shown as loading control. Arrows indicate molecular weight standards (St). Numbers show ALDH1L1 band intensity (arbitrary densitometry units) normalized to actin. Experiments were performed three times.</p
