Additional file 1: Figure S1. A to F, Repetitions of bright field, fluorescence and lignin staining microscopic analysis of the loquat flesh. Scale bar = 20 μm. Figure S2. A to C, Three-dimensional spatial concentration distribution of lignin, cellulose and pectin in the lignified cell of loquat fruit. Scale bar = 10 μm. Figure S3. Baseline correction using adaptive iteratively reweighted penalized least squares (airPLS). A, Original Raman spectra; B, Raman spectra pre-processed by airPLS. Figure S4. Raman intensity variations of lignin (1603 cm−1, black line) and cellulose (1383 cm−1, red line) along selected y-segment. The y-sampling was conducted in 1-μm steps
