<p>(A) The Venn diagram shows shared identification of proteins following BNP, SEC, and IP sample preparation. Total number of proteins identified in each technique is given within brackets outside the Venn diagram. The numbers shown in white font and within brackets are the final set of proteins assigned as subunit components of <i>T</i>. <i>gondii</i> F-type ATP synthase. (B) List of all genes identified in this work by mass spectrometry analysis. Gene ID and product description details are from Toxodb.org (release 36). The entries shown in bold indicate that the protein was detected with high confidence from only BNP and IP samples. Asterisk indicates that the corresponding peptides were detected in only one of the replicate runs for SEC sample. BNP data are a combination of experiments done with both <i>Tg</i>ATPβ-YFP-HA–and <i>Tg</i>ATPOSCP-YFP-HA–expressing transgenic parasites. SEC and IP data are from <i>Tg</i>ATPβ-YFP-HA and <i>Tg</i>ATPOSCP-YFP-HA expressing transgenic parasites, respectively. BNP, blue native PAGE; IP, immunoprecipitation; LC-MS/MS, liquid chromatography–tandem mass spectrometry; OSCP, oligomycin sensitivity–conferring protein; SEC, size exclusion chromatography; <i>Tg</i>ATPβ, <i>T</i>. <i>gondii</i> ATP synthase β subunit; <i>Tg</i>ATPOSCP, <i>T</i>. <i>gondii</i> ATP synthase OSCP subunit; YFP-HA, yellow fluorescent protein plus hemagglutinin.</p
