Additional file 2: of ATAC2GRN: optimized ATAC-seq and DNase1-seq pipelines for rapid and accurate genome regulatory network inference

Abstract

Figure S1. FastQC Reveals Tn5 Tags and PCR Chimerism. Non-random distributions of the first ~ 10 bases after tagmentation correspond to the Tn5 tagmentation after library primers are removed. This non-random region at the beginning of the read is characteristics of ATAC-seq data. In the ATAC-seq sample from Buenrostro, a second non-random region can be seen, suggesting PCR chimerism. (PDF 57 kb