The karyology of bream (Abramis brama) were done for the first time in Iran. This study was conducted on chromosomal number, chromosome arms and karyotyping of bream from the -Southern part of the Caspian Sea with preparing chromosomal extensions on the base of Squash method. The number of metaphase plates using Squash method on renal and gill tissues was determined as to be 2n=50 and the number of chromosome arms was NF=82. The prepared karyotype of this species was consisted of 8 pairs of metacentric, 8 pairs of submetacentric and 9 pairs of acrocentric chromosomes