Rhizome buds of ginger (Zingiber officinale Rosc. ‘Tambunan’) were sterilized and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of NAA and BAP hormones (1-3 mg/L) to induce shoot multiplication and rooting formation. Shoot formation was first observed on treatment of 3.0 mg/L BAP + 1.0 mg/L NAA after 7 days of culture. This treatment also promote the highest number of proliferated shoots, 6.14 ± 0.91 shootlets per explant, with an average shoot length of 1.69 ± 0.17 cm observed after 10 weeks of culture. Rooting of ginger plantlets were significantly initiated on medium supplemented with 2.0 mg/L NAA. This treatment induced up to 34.40 ± 1.81 roots per explant with an average length of 4.52 ± 0.20 cm after 10 weeks of culture. Plantlets were successfully acclimatized in pot containing medium mixture of sand and clay (1:4) with 64% of survivality after transplanted for 3 weeks
