Uji Aktivitas Antibakteri Minyak Atsiri Temu Kunci (Boesenbergia pandurata) Terhadap Bakteri Salmonella typhi Dan Streptococcus pyogenes Secara In Vitro
Background : One of the gram-negative bacteria that is endemic in Southeast Asia is Salmonella typhi. In addition to Salmonella typhi, there are bacteria that have a high incidence in the world that is Streptococcus pyogenes. Salmonella typhi has been resistance of antibiotic. Known to increase the morbidity and mortality of typhoid fever. Streptococcus pyogenes has been resistance of antibiotic because of the increased ability of bacterial adaptation to the human immune system. Essential oils of Boesenbergia pandurata can be utilized as an alternative source of antibacterials.
Objective : To investigate the antibacterial activity of essential oils of Boesenbergia pandurata through in vitro technique and the most effective concentrations of Boesenbergia pandurata’s essential oil as antibacterials.
Method : The type of research was experimental with posttest only with control group design method. The subjects of this study is Boesenbergia pandurata’s essential oil. Salmonella typhi and Streptococcus pyogenes are used as bacterial test. The bacteria test were standardized with 0.5 Mc Farland, then planted on agar medium. The media is made well and filled with concentrations of 30%, 50%, 75%, 90%, positive control, and negative control by diffusion method. Aquades as negative control, amoxicillin and chloramphenicol as positive controls. Then incubated at 37 ° C for 24 hours and calculated the inhibit zone.
Result : Boesenbergia pandurata’s essential oil has antibacterial activity toward Salmonella typhi and Streptococcus pyogenes in concentrations of 30%, 50%, 75%, and 90%.
Conclusion : Boesenbergia pandurata’s essential oil with concentrations of 30%, 50%, 75%, and 90% had antibacterial activity. The most effective concentration as an antibacterial is 90%.
Keywords: antibacterial, essential oil, Boesenergia pandurata, Salmonella typhi, Streptococcus pyogenes
