Increasing the use of motor vehicles in Kecamtan Serengan Kota Surakarta gives a big impact on the emergence of pollution in a region. The emissions issued by each vehicle cause accumulation of pollutants in the air. To make measurements on air quality takes time and costs are very expensive. There are alternative measurements by using a life indicator called a bioindicator. Lichen is an organism that has been used as a bioindicator of air quality. This study aims to determine the invasion of lichen as a bioindicator of air pollution in Serengan Sub-district, Surakarta. The method used is explorative with exploration. Sampling using purposive sampling technique. Sampling location in three stations is Jl. Veteran, Jl. Yos Sudarso, and Jl. Brigjen Slamet Riyadi. The results of the identification of lichen there are 14 species of lichen Parmelia sp., Canoparmelia sp. (family of Parmeliaceae), Caloplaca sp. (family of Caloplacaceae), Graphis sp. (family of Graphidaceae), Lepraria sp., Lepraria incana, Lepraria caesioalba (family of Stereocaulaceae), Dirinaria picta, Dirinaria aplanata, Physcia aipolia (family of Physciaceae), Arthonia sp.1, Arthonia sp.2 (family of Arthoniaceae), Lecidella elaeochroma, Lecanora sp. (family of Lecanoraceae). Observation on Jl. Veteran shows vehicle volume 19008 units / hour found 11 species of lichen, Jl. Brigjen Slamet Riyadi with vehicle volume 22603 units / hour found 7 species of lichen, and observation point on Jl. Yos Sudarso with vehicle volume of 31600 units / hour found 3 species of lichen
