The Garonne is the largest river in the south-west of France, and its drainage basin stretches between
the Pyrenees and the Massif Central mountains. Until now, no water stable isotope study has been
performed on the whole Garonne river basin which is composed of different geological substrata,
and where the water resources are limited during the dry summer period. This study focuses on the
Garonne river and its tributaries from the Pyre´ne´es foothill upstream to its confluence with the Lot
River downstream. The aim of the study is to determine the origins of the surface waters using their
chemical and stable isotopic compositions (18O, D and 13C), to better understand their circulation
within the drainage basin and to assess the anthropogenic influences. The Garonne displays a
specific 18O seasonal effect, and keeps its Pyre´nean characteristics until its confluence with the Tarn
River. The difference in the dissolved inorganic carbon (DIC) comes mainly from the change in
lithology between the Pyre´ne´es and the Massif Central mountains. Agriculture activity is only
detected in the small tributaries