The biocidal action of fique juice on the phytopathogen C. gloeosporioides, which causes anthracnose in tamarillo fruit was evaluated. Concentrations of 1,000, 10,000 and 100,000 μg mL-1 amended in the PDA culture medium were analyzed and tree levels of fermentation which were 0, 4 and 8 days. Mycelial discs of 1.1 cm in diameter were placed in different experimental units and the in vitro growth area was measured after a period of 8 days using the ImageJ software. Only statistically significant differences (P<0.05) occurred between concentrations and between the interaction of two factors. The Maximum inhibition of the pathogen was obtained by using a concentration of 100,000 μg mL-1 and 8 days of fermentation
