Endosperm protein and isozyme patterns of Unes derived from the ovoss [Tritioum turgidum (AABB) x Aegilops ventrioosa (DDMVMV)] x T_. aes tivum (AABBDD) were studied. Five bioohemioal oharaoters pvesent in Ae_. ventrioosa (DDM°M°), Ae_. oomosa (MU) and Ae_. uniaris tata (MUMU) , but absent from Ae. squarrosa (DD)t T_. aes tivum (AABBDD) and T_. turgidum (AABB) were investigated. Three of these were identified in several Unes3 indioating gene transfer from the M° genome of Ae. ventrioosa into hexaploid wheat. The wide distribution of one of the markers (CM-4) suggests its previous transfer to the D genome of Ae. ventrioosa