Effects of introgressed 4Nv Aegilops ventricosa chromosome on yield and yield components in bread whea

Delibes Castro, Angeles

Lopez Braña, Isidoro

Martín-Sánchez, J.A.

Moral, J. del

Perez Rojas, F.

Simonetti, Ester

Sin, E.

English

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i t e m s f r o m s p a i n 
u n i v e r s i d a d p o l i t É c n i c a d e m a d r i d 
Departamento de Biotecnología, E.T.S.I. Agrónomos.- C. Universitaria, 28040, Madrid, 
spain. 
A. Delibes, I. López-Braña, and E. Simonetti. 
u n i v e r s i d a d d e l l e i d a 
Departamento de Producción Vegetal y Ciencia Forestal, Institut de Recerca i 
Tecnologia Agroalimentaries (UdL-IRTA), Rovira Roure, 191-25198 Lleida, Spain. 
J.A. Martín-Sánchez and E. Sin. 
c o n s e J e r Í a d e i n f r a e s t r u c t u r a s Y d e s a r r o l l o t e c n o l Ó g i c o 
SIDT (Servicio de Investigación y Desarrollo Tecnológico), Ap. 22, CP 06080 Badajoz, 
spain. 
J. Del Moral and F. Pérez Rojas. 
Effects of introgressed 4Nv Aegilops ventricosa chromosome on yield and yield components in 
bread wheat. 
The wild grass Ae ventricosa is an allotetraploid (2n=28; genomes DvDvNvNv) and has attracted considerable attention 
as a source of genes for resistance (RG) to pathogens such as insect and fungi. Genetic material from Ae. ventricosa has 
been transferred to hexaploid wheat through an intermediate self-sterile hybrid between T. turgidum and Ae. ventricosa, 
which was backcrossed using pollen from hexaploid wheat. The progeny were repeatedly selfed to obtain 42-chromo-
some, stable lines. One line, H-93-33 (4D/4Nv substitution), carried the genes Pm and H27, which confer resistance to 
powdery mildew and Hessian fy, respectively (Mena et al. 1988; Delibes et al. 1997). Introduction of these RGs from 
line H-93-33 into the commercial wheat cultivars Adalid and Astral was by backcrossing. Marker-assisted selection used 
the isozyme Acph-Nv1, which is linked to genes H27 and Pm on 4Nv chromosome (Delibes et al. 1987, 1997). BC4-6F4-
BC4-6F9 lines were evaluated against Hessian fy in Azuaga (38º14’N, 5º40’W) from 2000 to 2006; and BC4-6F6 lines were 
evaluated against powdery mildew in 2002 in Gimenells (41º39’N, 0º25’E). Lines with the Acph-Nv1 marker were resist-
ant to both Hessian fy and powdery mildew. 
These lines, with and without Acph-Nv1 marker, also were evaluated from 2000 to 2007 for grain yield in 
several Spanish localities under irrigated and unirrigated conditions. Averaged across the different genetic backgrounds 
and 18 different environments, the 4Nv introgression decreased grain yield by 17%. The effect of 4Nv introgression on 
grain yield, yield components, (evaluated as described by Bell and Fisher 1994), and quality was studied over fve grow-
ing seasons (2000–05) in Gimenells under irrigated conditions. Averaged across the different genetic backgrounds and 
years, the 4Nv introgression decreased the fertile spike number/m2 by 12.8 %, and kernels/spike by 7.8 % but increased 
kernel weight by 9.3 % and protein content by 12.4%. Bread making (determined by alveograph parameters, W, P, L, 
and P/L) was not affected signifcantly by the introgression. The isolines also differed in heading date. Lines without the 
introgression were 1 to 2 days earlier than those without. 
The effects of H27 and insecticide treatment for the control of Hessian fy were compared. Three pairs of NILs 
differing at the H27 gene were evaluated with and without insecticide Diazinon. The feld trial was conducted in the 
2005–06 growing season in Azuaga. Hessian fy damage was estimated visually by incidence of broken tillers on the 
second spring generation. The effect of insecticide on lines with H27 gene was not signifcant. Moreover, lines carry-
133 
ing H27 gene had a lower incidence of broken tillers (P<0.01) than respective isolines without RG, thus H27 was more 
effective on the control of flies than insecticide treatment. 
The effects of Pm and fungicide treatment to control powdery mildew on yield, yield components, and quality 
also were compared. The same three pairs of NILs used above, which differ at the Pm gene, were evaluated with and 
without fungicide (Cyproconazole plus Tiophanate-methyl). Field trials were conducted in the 2005-06 and 2006-07 
growing seasons in Gimenells. Grain yield was 35.8% greater in 2007 than in 2006. In both years, treated plots yielded 
more than untreated plots. The decrease in yield in plots untreated (in relation with treated) was lower (4.7%) in lines 
with the 4NV introgresion than in lines without introgression (8.7%). As expected, the Pm gene had some effect in con-
trolling disease. Protein content was not affected by fungicide treatment, but it was affected positively by the introgres-
sion. Bread making was not affected by the introgression or fungicide treatment. 
Acknowledgement. Financial support for this work was from Grants AGL2004-06791-CO4 and PET_2006_0424 from 
the Ministerio de Ciencia y Tecnologia of Spain. 
References. 
Bell MA and Fischer FA. 1994. Guide to plant and crop sampling: measurements and observations for agronomic and 
physiological research in small grain cereals. CIMMYT, Mexico. 
Delibes A, Lopez-Brana I, Mena M, and Garcia-Olmedo F. 1987. Genetic transfer of resistance to powdery mildew and 
of an associated molecular marker from Aegilops ventricosa to hexaploid wheat. Theor Appl Genet 73:605-608. 
Delibes A, Del Moral J, Martin-Sanchez JA, Mejias A, Gallego M, Casado D, Sin E, and Lopez-Brana I. 1997. Hessian 
fly-resistance gene transferred from chromosome 4MV of Aegilops ventricosa to Triticum aestivum. Theor Appl Genet 
94:858-864. 
Mena M, Orellana J, Lopez-Brana I, Garcia-Olmedo F, and Delibes A. 1988. Biochemical and cytological characteriza-
tion of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV. Theor Appl Genet 77:184-
188. 
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Effects of introgressed 4Nv Aegilops ventricosa chromosome on yield and yield components in bread wheat.

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Effects of introgressed 4Nv Aegilops ventricosa chromosome on yield and yield components in bread wheat.

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