Bigirimana, Vincent de Paul

Demeestere, Kristof

Haeck, Ashley

Höfte, Monica

Peeters, Kaat

Ghent University Academic Bibliography

Toxin production by Sarocladium oryzae, the major sheath rot pathogen of rice Kaat Peeters1, Vincent de Paul Bigirimana2, Ashley Haeck3, Kristof Demeestere3, Monica Höfte1  1 Ghent University, Laboratory of Phytopathology, Department of Crop Protection, Coupure Links 653, 9000, Ghent, Belgium 2University of Rwanda, Department of Crop Protection, Musanze PO Box 210, Busogo, Rwanda 3 Ghent University, Laboratory of Environmental Organic Chemistry and Technology (EnVOC), Department of Sustainable Organic Chemistry and Technology, Coupure Links 653, 9000, Ghent, Belgium  Session 3: Microbial determinants of pathogen and symbiotic interactions  Sheath rot is a very aggressive, emerging disease of rice. During the last decades, the disease has spread all over the world and has the power to destroy the total yield of a growing season. The major pathogen causing this disease is a fungus, called Sarocladium oryzae. This fungus produces at least two toxic compounds, helvolic acid and cerulenin. Helvolic acid is not only toxic for the plant, but also for other bacteria, while cerulenin is toxic for other fungi. According to the literature, exogenous application of both toxins can mimic sheath rot symptoms, but a clear correlation of these toxins with disease severity has not been demonstrated. The goal of this research is to study the diversity of S. oryzae isolates from Rwanda and their capacity to produce toxins. Therefore, we measure helvolic acid and cerulenin in vitro and in planta using LC-MS and study the genetic capacity to produce toxins using PCR with specific primers. Toxin levels were correlated with the pathogenicity of different S. oryzae isolates after rice inoculation using the grain inoculum technique. Results show that S. oryzae isolates differ tremendously in their toxin production. Ongoing research will further elucidate the role of these toxins in phytotoxicity.  Keywords: Sarocladium oryzae, helvolic acid, cerulenin, sheath rot 

Toxin production by Sarocladium oryzae, the major sheath rot pathogen of rice

https://biblio.ugent.be/publication/8566287/file/8566289

Toxin production by Sarocladium oryzae, the major sheath rot pathogen of rice

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Ghent University Academic Bibliography