Foetal bovine serum (FBS) induced cytostatic effect of peritoneal macrophages against mouse melanoma

Abstract

Peritoneal macrophages (PM) obtained from normal unstimulated adult mice, from several strains, exhibited a non specific in vitro cytostatic effect on Harding Passey melanoma (HPM) cells. PM lysates and PM supernatant culture fluids were separately assayed. 72 hour supernatants were found to contain a strong cytostatic factor, which was heat stable and dialysable. 72 hour lysates were ineffective when taken from young mice PM cultured in the absence of serum. A strong cytostatic effect of the lysates was produced when the same PM were cultured with as little as 0.5% FBS.SCOPUS: ar.jinfo:eu-repo/semantics/publishe