The absence of menstrual periods for one or more periods is called amenorrhea. Primary
amenorrhea means the lack of its occurrence until the age of 16 years. Secondary amenorrhea
refers to situations that a person has experienced menstruation periods in the past and then stops
its occurrence. Amenorrhea is a symptom, not a disease that can cause due to disorders of
hypothalamus and pituitary glands, disorders of sexual glands and deformities of the uterus and
ovaries. Due to the clinical importance of reproductive in life and non-reproductive of women
amenorrhea due to ovarian function, this study has been conducted in order to determine the
prevalence and type of chromosomal abnormalities in women referred to the Cytogenetic
Laboratory of Cellular and Molecular Research Center, in University of Medical Sciences of
Qazvin. Karyotype analysis and its relationship with phenotype were performed on 110 Iranian
female patients with primary and secondary amenorrhea. Metaphase chromosomes were
prepared and analyzed by G-banding technique and were evaluated to examine the mosaicism
of 100 cells of lymphocytes cells