Sharifi, Maryam

Simorgh Research Repository

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";L. ..Kerman University of Medical SciencesFaculty of MedicineIn Partial Fulfillment of the Requirement for the Degree MScTitle:Er alua'ritrn bet*'een Entamoeba gingivctlis and, Trichomonas tenax in highscrt'oi students of Kerman city and association of infection with demographicand clinical factors by culture method and pcR in 2017Superv'isor:Dr Hamidreza Pouresiam iAdr,iser:Dr Fatemeh JahanimoghadamDr Zahra BabayieResearch and writing:Dr Maryam SharifiAcademic Year: 2017-2018 Thesis number:147q$rJ*3c;r;e dlA ajLjj.t l+ijl l,- ..b n 4srlrf- tl rs** ol-fu--j ,;L;l ;Lnr ol.,:esaa g c.orl.oa-f c-",1 r-&.,,1** tfy;-l c-l ,:!,,*..rrtil .rta ,t,-*jti 9l) LS)La.5l-o -9f*" a)*,f.[,:'o.;;;lo:.rlrl, L_r-!)l 9l;,- a altt- ;_l .ir- .-,Ltj; d.y; ,f +*y)1 s a.y.fi,:-U at ;to n,*;l5rlrr(-6tli LL4) ,s":y , AU.5 fi a.9> aJ*y- +-rr" f ;l;9.1 ,pl: ,: ,JL' o"Ls.#_i 9 ,J|y-;; L-bl.s el+l.PCR ltjl e r:S sla G, L tJ-<;*I' e s-slf y:,J"t* t,\A .r;- J-foL-) ;l^-t f tO J.:L,-:.:^: rl .-rIJ;' 9 & Jsy- ! dJtt a,l :b cl"l.t I Jlf.rj, ; uL-:Jl ,t,J,*., d-p j9tL,,.._,L,,r:) f ,l ai r;),, F) ('/.f ,) \\, s *.('/t.) \A1 &Li,(JL,l;i;Lsr o,-L a-;L c--; *Jt Jsf ot tql ..rLt.tr.--L,r 9 ,l"s4x s ,-;"):,1 :y-) g )f- tl ;l ,-.stL d! :rlo a-;c.,; .(-- .r; ali.s oir- 9r .gl a! ,i .(),tl. r1 JE9r9,-; J.r-l orf ll or[::,.,1 \ t fr.F+>rr YA.5L: ,yL9-(1 ,,) , $ o;1.: eJ 9 e^i.[' -l 7 -, c*3 to. a, il) d]- 9 :,; .f,_,,;1 fu.; Jl*. )rc; ,\p i,+- c<,;l )sg" PCR Jjr) L ou orl: cJ .116 o:9* .u.S rlp tlf ;L;11-*" .r--:y ":pi ,*.ilr_;j L"i;Jl q ,;.) (l r alf ;v e ,-- (/.x I t) ry .._rlpe, f f .6e->- ,r :[a aJL),J*+ &L- ,r1r ;- ,9;5G y."*; &!Lt .ri)er c r,i.c pCR t ^i9- ( /:\f/1) ff "t Jt ,: .riri .i_L|;;& t-iia r+ Jr- .(p<. . \ ,*; Y ,. ,, ):lr ;L; l, l-r,_slS .s[. +;f 9 r,-r; .7lafl fr .Sl oJ d^>L" ,(rl,*.:r,-i-i:1 qJl tr.-rl d o.>Li &U "t.,5 *; lr rl:;* *; y q;-,"1f d9-ri- S6 ;#b jt.5l-.Jry ( p</. tf ) 1:,-sl5 .slo *e< e (p<.1 .f),-l- .StA a;sL_ :rg 2$ a4;:,*l ;*1-"L; Ll .-r; o r,_: .r*,-L_ o ,: ,Jb' ,yiyy<-y q ,jrdi a.rSl .i--l c;b ;L; l, .r<;J5 9 -(sl S,*:9 r;L-l) ,- -il+a e+.t.b, d+ r*;-;; + phsl L;;19i t' ory olSi Gr> { -)L(; ;ql ;l _r,-! ,;l_or c_-itr_;V! i"- r: ..Jb9:9r-- -V(;._rJL_,#lo C a Lsfu-, r: u-;19, .r. ;L1-uil;_l .-;-L;._,Llr:t r) -,i*:.JiJ _<*.o"-y-fi,4L*,9^o 61;9.i .rils ,t3*; ,u,&U,yUy-*.1 1f-Jllrj t,y."li/:6.t 5 olnsAbstractBackground and objective: Epidemiological findings indicate that E.gingivalis isa colnmensal protozoan parasite which possesses high capability to become opportunisticpathogen. This study was aimed to identifr the prevalence and potential factors that areassociated for E. gingivals and T. tenax in adolescents in southeastern lran,20lJ .Methods: Overall, 315 adolescents (mean age; 15 years) consisting of 189(60%) maleand 126(40%) f'emale u,ere selected from 4 schools in suburban areas of Kerman city. Afterobtaining the authorities' consent and willingness of the adolescents, they were clinicalllexanlined for oral cat'ity complication and a specimen was collected by scraping debris alon_ethe gingival line using a sterile curette. The specirnens were cultured into biphasic media andincubated at 35.5tic. Cultured sanrples were further examined by PCR technique.Results:Totally, 44 adolescents were infected with E. gingivals and T.tenqx. Thc rate olE.gingit;alis inf-ection in males was 3.2 times higher than that in lemales (p. o.oot)" CultLrrernedia detected 10.4% (n: 33), whilst PCR idcntifled 13.9% (n:4,1). Univariate analysisidentified 3 rnajor I'actors inclr-rding sex(male). sinsival inder u,ith sever inflammation anclCandida spp (p < t).001). Mullivariate model conllmred onl1.2 principal deteminants narnelr,gingivai index rvith severe inf'lammation ( p < 0.0-1) and Contlicla spp (p < 0.013). Factors notassociated r,vith E. gingivalis rvere parents occupation and histor,v of systemic diseases.ConclusionThe present findings clearly demonstrate a positive association between E. gingivalisand demographic and clinical risk deternrinants. Therefbre, dental practitioners and healthsurveillance personnel should be aware of these confounding factors to rigorously detect andcritically manage oral health issues in school age children in order to prevent or at leastnrinimize the er,entual periodontal complications in later lifb.Kcy words: Entamoebct gingivalis, Trichomonas tenat, Prevalence, Middle schoolstudents, Gingivitisirb-It .sii;' pgJc ot1i.ilo=li;il.tjs c*$ilso , .,li( tsJriallri n"u irb-qjl 6tio &o2f >lli1 5.e.iii €1.#s +;3.r c,iL3.r 6l;r c 4u,t fg-l rS.r pt= <-"t: drtilq jl at-iJ Jl t:-" .r,rl"i .,lr*i -gl.igoT ,.f,10 gbs Js .J.5gi rrligl*j r .l{le$ij l+*"tiil cirlff;g s+r1y'rrls-,c ,-,-r Crl5ff .##hir;" " lfti Jt* :s PCR 9 r:.i.t ,JigJ q :f*Ct g *il.frs .J"f* tf ,JsgrT 9$rf 9 gt -f Ji d&.9i" .rrfr.lr .rL!f c.ti 6qq yi cs ulJglo s$ .qr-S rl$r lYll ll7 g1-,8 ! ,-si 1sL otLuto ,r<;..lgls.r 6J<.slr+.li 6lil{ . .rji6 o,o tkl,r*.1 CJ.i 4+ lJ dsij 6L.ftljl pjY igta;.,,€ J cr$16.r U.!r.i jt qr* .+rl o,r3'o3 dr-E ..cu.,l oo3,cr Short; Cb j!3.ol q lA /YA of.r o,:;,uLr g cu,rl JS+ rJg",rJr3 cr.re;-r - -gll9lo el1lL.:o11 rL-)9u!" li,.-.5.:b.- cilrLJl a^tti ;f:,g_ 1;r"r^+y5:pri" .f\a 4"tE r"5ii"t4\a-;r;5:6cI1 .g;.rJl*,t&xqg.urU r.or ,'ria9 *-'.IJ VJi<t-tulft d9P .e-;,*)fi o9f ,r,a.a><; Lu,st.*or5;;ll G."-t J (ut t.*e:l ol-:9S"ro a4-L.rlrxJ ci ts'o:11 "r 6il >t-.,u _5J-.rV-I;X L;;.r.-t yfr

بررسی فراوانی انتامیبا ژنژیوالیس و تریکوموناس تناکس در دهان دانش آموزان متوسطه شهر کرمان و ارتباط آلودگی با عوامل دموگرافیک و کلینیکی به روش کشت و PCR در سال ۱۳۹۶

http://eprints.kmu.ac.ir/27297/1/6539.pdf

بررسی فراوانی انتامیبا ژنژیوالیس و تریکوموناس تناکس در دهان دانش آموزان متوسطه شهر کرمان و ارتباط آلودگی با عوامل دموگرافیک و کلینیکی به روش کشت و PCR در سال ۱۳۹۶

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