Sanaee Nasab, Amir

Simorgh Research Repository

;t,s;'5 dk:r c*lxri j "ssaiti pSJr ot<,i,ilr,dirl'l f.jLs * *jL*S-rl'l 'r":s'li*l'l'r-,5,*: ga*rl*;t* *L&-dL: rS.r*.<''t*rq '1..r <l j.S; a-.rtr ''l.rt-rvJ JJ v J V - a:,.1lJ"rsItT{{ I 07$ tp4prt /r*LiS"S;J* ,l$**!J,,,rrg:l3,$* q#l J jS*t*;,r &r t3$l$J$.,*,,# rlT",$;&?.*r] o*L.l*; 3.r*l:L*i*}, {:t*,1utB*"r L&yll& ;ii;,Jig.&o ;rll.:r"J,l.a.i JJ,SJ{14 :s.&t} *r*k *.,rl*i lft'l-{l&.,$lHFI-5d,Kerman University of Medical SciencesFaculty of PharmacyPharmaceutics Research CenterFor The Certificate of Pharm. D. DegreeTitle:Bioencapsulation of Biosurfactant-Producing Pseudomonasaeroginosa PTCC1074 in Alginate HydrogelBy:Amir Sanaee NasabSupervisors:Dr. Gholamreza DehghanDr. Mohhamadhassan MoshafiFebruary 2018 Thesis:968Abstract:u-1$ a+}i.J,i G>L*.-i5 .iat5 ;rt JJli .r" a5 -u**" -<;! "r-i .,,-bJ:-{_1, c;151-iJJ* :ar.te.,;: t\t\; .rL," 6Lar,,-,5 Ui +5 ;-l o-u; c-,pL c-iUSt-iJf )f a, )--a>i SG 6 i:.r.:-< l* o;-,,, : g_tJi .,#lJ+ -clr o:;."ii ,&i,Jy -.G -.-- .:#)* JL^+iiL*; ai -u;-r r":-- ..St^yt_i" g_,BL c-,[i:i ,l .s^:-S y ";L:" &i[S\::-s:;, ,;_t*tj c-JkSt-i)J* ,Sll :*S l-r1-4.-(- olr" t : $_r. ./ 4-r g;1" --;L(rtr.(-..t"S ,rLr ;;LSG;-1- \ o^-\i. ;: .-lil o:r5 l-t- 6l "> ;3 ,;La;;;t5 -rr-l +-^: 5.!! -c;-1, :--,$ -.--, .r-< J,=* "l - ;l .-tJl:;;.t ,Jt:lt j[r;- -'t L^ --JL:-(r...*.-o3U;." &LA d^tl; e r-iL;"" .9t^pH ,41.: \l;;s olJ d-Jt^i J L?*L. ra 6;K3l-: S--)-):: glo ":r3i; JjL :lji ): )y*" gG ;t); jl -,i" J+ J+ .;,.t ;t -<^;Jl;" rrl;.ol 4r r)e> -,)6F! grL;:l-u SF:\ ! y {.L:./ ell 1i -u.iL J; rU;U.rS.r".-G c;LSG)t** 4r _, "*.1;5pseudomonas aeroginosa (PTCC1074) e ."-r- o:rS c-*:s rl o:t,i:-iL -*,,i'j';,,i ;rJ)j! .rJtd-l -;.l;.ol ,J- ^r Jj3,, 6JL q,fi ,3.t ,,*.. 3 .--,1,-;i f^-IS .5LA Jj-r-,-L^ -::6t^ JJL Jt- C c-----i .o-tj ;".:J J-Jt-- ;r 1; _l.aL- c;[SGjr] ;lt5 y !;,.r*(,l,g -,J)r'oJ. J ' Q 'J. JJmAbstract IV:;y j*;*.rLeL j) u,y ; -tj-u orl: *t5 rlri 51r- -,, L" 6F! ;i l-uil :La j-l.l-li c-5 -t,=" c^jtju /,!- 'fu "riS ,optical density ".L-7 ,1 i-$V-6 gL" .tL-j-,I-+ drL c-:e 6l;t ,L" *-S -,15 q -Qli a"-f< dL^+ ;: c--5 tS otr-,ulrl J) .J5;,-?l-b c-rrs )l,oL,-;;i g..5.gta,r- ,> P. aeruginosa (PTCCI}7a) ,g-\ts\ ts *-y...r; ,-,EJl 6rLi J-r, y G o>1: .-*Ll, ,r--L-,i J.* 6_ | -)J-;;r_l -u o:Lt-l -.._L-,i,*:* j-l^ .(- ,c--U ,') 3 o:l: ;ll -*, lfi tt-r l-, L: ;L;1 CU .r"r* s S ,S Fl+jt' L^+ ,6"* J d*.:;"-; crl...r_p c/)J +5.:*l -5r.. p;Y..lo: .,,. {*"Jl '*l-s-.1 r - r-J) :t j ,1L-j-,I ) qi:;-u Ls --,-Ljl ^L.jl jl --*r :;^5 +lt *:-5rl "r*1 lql P. aeruginosa (PTCC1071) 6 \s- rl ,Jl5U-,r_r 4r:to a:iL_rL-))l : S.J o-.r-Ui :;;r,---Lji ft);) o-L-i ^jl;l u or.-- --Llrl ,r?- ).t;i,SkJ-rL-*J5 .:J"-l; t- G\t 11 *.;L-!i g-u- c-[-1" r-,5\t 4 e-- +-( -r; -&-l.-"-r SL- o: +5:;r: ol r*r ! l, n-L- i.-{i.J); e",lLr 31, ".q-i: 15 7,L- j xl)S..:1.: .rt.;i rd* "i^6 t+i .:Jt - {r *.1a" s: oS 4l mN.m-1 .rl;* q c:,.L- J;;5r:F o, P. aeruginosa (PTCCL074) 6 \_r- 6 .:1.: ,tl:.j crUt!, ;,1 :r9r$ *a*;*-; J-l i;t.SUJr;l. 4i : )J, i.,!- Li- a.;rt-!..,1,-!i s^-J5 6Lo-r.-;,: or: ttL--l.{-_, ,bl_i- c-J[sGrr] !i rl'r.L -r-/* ^, c--o\24 ;l..-LS[!)r;. , *l)t])r.J,q]1 +,J).5 +tt-l :6,rJ5 drL^15AbstractAbstractBackground: surfactants are amphipathic molecules that Reduce surface tension. Theunique properties of surfactants head to a vast array of practicatapplications.Which are illustrated in terms of petroleum processing, biological systems, healthproducts, food, and cosmetic protectants.Biosurfactants belong to a structurally diverse group of surfact-active moleculessynthesizes by microorganisms and their applications have been greatly extended overthe past five decades as improved alternatives to chemical surfactants. There are manyadvantages to the biosurfactants as compared to their chemically synthesizedcounterparts. These include lower Toxicity, higher biodegradability, betterenvironmental compatibility and specific activity at different temperatures, pH rangesand salinities.Cell encapsulation resents one of the current leading methodologies aimed at thedelivery of biological products and alginate is the most frequently employed materialfor the purpose.In this study, the affempt was on using Calcium alginate hydrogel entrapment methodand entrapment optimization as well, in order to increase productivity and efficiency ofbiosurfactant produced by pseudomonos aeruginosa, in comparison with free cells.Methods: the bacterial free cells were cultured and then at definite intervals, variousmeasurements were evaluated like optical density (Ez+, foam activity) and surfacetension of culture medium supematant. In following, the entrapments of calciumalginate beads were used. In order to have a more precise statistical analysis of theresults and providing a valid mathematical model, the experimental design method tooptimize entrapment. The technique was used exclusively for this particular strain. Theresulting data were put together and analyzed. It should be noted that the morphologyand physical properties of beads during the culture process after competing experimentswere evaluated.Results: Biosurfactant produced by P. aeruginosa (PTCCl074) were evaluated afterfree cell cultures and then after culturing on the basis of a method proposed by design ofthe experiment. Using data obtained, a validate methodical model was achieved. TheAbstractlevels of three variable, Sodium alginate 4oh, Cacl2 lYo,hardening time 15 min werefound to be optimum for maximum production of biosurfactant it was given the surfacetension 41 mN.m-l which compared to free cells.This study showed that P. aeruginosa (PTCC1074) entrapped in calcium alginate beadsis able to preserve its viability and produce biosurfactant as a secondary metabolite.Maximum biosurfactant production was achieved during the first 24h.Key words: entrapment, alginate beads, optimizations, P-aeroginosa .II@.",*,f *t srb'ri s r# t p It ol$iiloolrf iblos91U91lo c.t$iilollA oltoi q jlo, s-r, t. ,S09r, g1b91lo rg9+iilo lnr .-ttl,r J*.1 6GT ll0 gQlr,:glri, c.rti' * e1 TJ) u a ; i iC t r,t rP t CCL a 7 4 u i ;Tu t r D r g r tt /t r,'' - 't " ' ,hjir,#t.,r\+Y" f....... (;-r-' l-') .F"; \ e '--s,r ,s* 1t-i1!_-tY/1/YV e-ru',,r/ .*-t *-y- + ...f-"(,,1& *r"Sr.r)..(.+r \)"-" I/,t'( otL!'i.?e-/:stsff y{rjq C g.,lp[)Ldl.sqr,/FY6)9P oJL ;51 -f,t, -'lrlP'rre;'o{';'}*ffi:.-:'..I;+4'

‭‮بیوانکپسولاسیون‬ ‮سودوموناس‬ ‮آئروژینوزا‬ PTCC1074 ‮مولد‬ ‮بیوسورفاکتانت‬ ‮در‬ ‮هیدروژل‬ ‮آلژینات‬‬

http://eprints.kmu.ac.ir/27323/1/233T.pdf

‭‮بیوانکپسولاسیون‬ ‮سودوموناس‬ ‮آئروژینوزا‬ PTCC1074 ‮مولد‬ ‮بیوسورفاکتانت‬ ‮در‬ ‮هیدروژل‬ ‮آلژینات‬‬

Abstract

Similar works

Full text

Available Versions

Simorgh Research Repository