Seied Hosseini, Seiedeh Elham

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6L"Js stb.ro .":.iloa.r gLoli J ..rsrii f3Js 6l(,nif.lcsi3;l.r fSbS cfjt*S.lf u o.rS.iilo,j:.,-, O u+t1( 6La3-,11.1 43ltri^'i 5-*(J;jL*JJh (s.Jrs, 6J3S.t d.g ohq:0lJrs6La uj*,,61js ,r5*iis Jt+o cri:ta JIl.r. &qr3 J JhJlsl+ ,iiI ol;31 .*l*j';lif-F S.l S* gCydonia oblonga MitL ,".1" ftr t+ q .5; 'ir.lr.o: L...Ji,".iiJrrt 'li'* fl#l oJ-rgr:lai'al; gluL!,.1!fl+J4.o l.t]i.;3S.r!F..rao i,r.ss J..s,6 3iSOlVf ;a.og _ibq o;La.i \rlv JLsrffiFtl-dKerman University of Medical SciencesFaculty of PharmacyHerbal and Traditional Medicines Research Center. Pharm. D ThesisTitle:Evaluation of antibacterial effects and determination of MIC of differentIeaf fractions of Cydonia oblongaMill. and bioauthographyBy:Seyedeh Elham SeyedhosseiniSupervisors:Dr. Mitra MehrabaniDr. Mohammad llasan MoshafiYear: 2018 Thesis No:974(-s!Jt! 4-)Li,ot5>g5t" qgJt * )-jn.e ;: ,l-s:\t ,)L^L< .;$-,, 3 -(;:; ,-gGy;i: 4x) uc:\f clc a, o)s-tlJGt< ) SF o,*.*s:\,e-J)\).-,;J-b,U.-,ljl ,:ats J jS-",,-5rp\,J1L-r:Ji -iI;,JiUt 2Ve ";:* J,p Jl;l &itl" .-ll ;: .g-l ctLL; 4*K jl "rt' p ?L J G-illtpf *r f &u ,ulsfu i-F A /.lt s> ,Ki ,->, Gs-t 9MIC ;y;;1 o:Liil L. * 3-t.r.:-, ! ;i t;-t ,'r;4Jl; C--tt-t" j,::-, jl .-t J -Lj ft+l ,* ff *--r- f y ;:-".+r.S 4,JU" :* rptf il-*"fu<i L--r s-;*,tJ glii eq 6y),-rLi; +;L-;1.t, jUi-,: ! o!( 3r.|26 sts.ss L:f -E!.; jt F )Li ;: ;,.: L,; 3 or"i c*-L ,6 3 -r Jit i o;Las jJ*-lJ,-tq .L( 3 t. u"* j-; ,>lrata; .+r; ,.*t-." "6 t)s a, -(.^ii o;l.as J.4)) J -u; "(.;-t'+'; r/-)-r -tor[aLl \y*n c,13,e X;\f ;l- -l;r-;J,-l: ,S\ -;r-, j: ;\ gjf1" -r) a\;l "f-tl. rS\;JJ+ )-r,!r*l L--u;s: J-:*[; J)[- t) .s^6 ":loo l-u,i ,i(i $:.rs:;).r:4J.r) S 7Jl: h-r-- a. JJ,U.<" i ./\ i-tle l,d.JS.1,.trj[- y,r=-.J*: JjJ Jt<i. - J (_- r I e.JJ - -J) a J !/ r,;l-SylX. Gss :: .'L; o:Li:-l MIC ;rr,,; ) * -j1=" e(La i-81; :..j-r _r_l, \. , ./\).pl "<+*IJJJ-i 6y,.ih-l #l ,J_r:t, Jy- 6..5 *.,?g o1l,,as 4;t'o,lr*-St;J".-.";; TLC .-,i-=-c..a ,-r:1.:;lj jl Jn .u.g;Ll-t- .5;Li a,) et_Si\:f ,-i;-, !: (,,'\..tt-L; q!-rrly' *-,* .(J a, Gyfu.r;-, f& o( .ur*t,-61 ,JLj * : ,s:f--* .X+..l,-r_S o! Rr a_:p ! -: ,*t's-JLi 4-)i--f f !*u-J r-*t5;'-0.5"" u Gj;..i .,,-t-F! L; it;t,;yI,-lr .6i ,.:;;: :6LiJt+" cl,[l #lo:^y;, !,.*y_l "-_$-S:f* j ry_i-:s\,.-f_f**l ,..,---r.1;{3i,t-\ tss-; -r 6 el-S;i- 5;*;:.r1r iL;. jlJ Jn t d ,* rY,2f ,\2 J:[- ;itsrr .-r-J:"-:*\ t*-f -f -l*l:-l ,:/Jtsf * u*;tt G)fu D ;1 .;jr--r= pte.,l if uo:3t Jt -l $t or;t;+5 -t..: rp;;..:t!1"r1 pt+;l;l 1,,.rl: eL:-J Rr: ./0Y o)3-b.:6 c-l -r-r c)p -r: ki €-Fj.r_F_6:t r,.ri-:s\o"-6-f*Lt s\.:f* *_,!,uA -t\t=S-5!1lr;_i ,Sl-tE; p-r.c+JLal-tJ.r:-l[a:ii:,'-...i;*;1 -a,5 1;1 u-a.L:. o-LoL:r ---L;i;'F;;;J,_1: tKi ,;tf g\_*.,64_rs)Li .ilrfjr ,r St:ojl3 4J5t1 -,..r+]}rl 4sitsAbstractNowadays, due to severe side effects of synthetic drugs and therapeutic benefits ofbotanical compounds in different diseases such as diabetes, depression and migraine,the value of traditional medicine and plants is more clear. In this study, antibacterialeffects of ethanolic leaf extract of cydonia oblonga (quince) was evaluated on four G(+)and four G(-) bacteria and after most effective fraction detection,its components wasalso studied by bioautography method.Methods: Quince leaf was gathered in November from Razavi Khorasan region andethanolic extract was prepared by maceration technique.The extract was concentraledand dried by rotary evaporator and dried content of plant was culculated. Phytochemicalproperties of quince leaf were also charactrized.Antimirobial properties were evaluated by agar dilution and bioauthography techniques.In agar dilution method, plant extract was dissolved in proper solvent and was dilutedin sterile Muler Hintone culture. Then the concentration of the 0.01 of microbialsuspentions were applied on the culture media; different concentrations were preparedand used for MIC determination. In bioautograply,. plant extract fractions wasseparated by methanol, Ethyl acetate and TFA ( 0.1, 10 ,0.1 ) TLC solvent system. TLCplates, then were put in microbial culture and no bacterial growth spots were clariyiedby tetrazolium indicater and Rrwas obtained.Results: In agff dilution method had antibacterial effects on Staphylococcusepidermidis, Staplrylococcus aureus and Micrococcus luteus with MIC 16, 64, 32mg/ml respectively was show. In bioautograply technique on the same bacterial strain,extract showed obvious effect againt S.epidemidis in Rr: 0.53 after experiments,it wasobtained that, Ethyl acetate fractions quince leafhad synergistic antibacterial effects onS. aureus and M. luteus, only when all components are in sample. Therefore, no-growthzone for these two bacteria was not seen after several repeation of experiments.Keywords: Quince leaf, Flavonoids compounds, Bioautograply, Agar dilution,',L J , i[o,oY ' VTreHLEEU=I.t.if** sb'rii J =SU;4 P5& e(f.&ilc{.Sit*g,rto ou$itotYf c,,t".i t t. r.gs-::.g 5g;&glfo $rqdifo €+.ri# fkllo*r+ p$ a*6;{Q:gfg;s C,*iCy {o n { o u{ r i t ..i. ii g t t # fril *i, J/(;i u ) r r}.1j, t "-J' 4" gt ,jt)'ii r,t ofifongaMt[C,:L;rt; **I{*,tGL.* lr*";5r -\/!al!->J*2r ,.): -)Jj a; gt;gtc Sgs* -4#X ,.'nt tJ, -1./J . -aJ -; LJ !9 J) )-'l/9-/..tJ/.il..1-p6lg:- 4r!t-, s) J - l-1. .-/- €r .-Ar rU !-0i u) J - l./\ J /\ /$ ( ?...... (.* !) .,*i ''-. e c-s,s,,l. ; .,1* .1;1 ,tr 4,V l't,'tf 3-.:- -':)f, , .-^1-.7 3-.c.i , Yl (t*l U* )O.z).' -i...> -,IO:-tl_-{ -g-;;-* _vst, \SF/.?t\N#lk?X*t\"qI

Evaluation of antibacterial effects & determination of MIC of different leaf fractions of cydonia oblonga Mill. & bioauthography

http://eprints.kmu.ac.ir/27290/1/6535.pdf

Evaluation of antibacterial effects & determination of MIC of different leaf fractions of cydonia oblonga Mill. & bioauthography

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